Perdita E. Barran
YOU?
Author Swipe
View article: Mapping the Conformational Landscape of the Cold-Regulated Intrinsically Disordered Protein COR15A
Mapping the Conformational Landscape of the Cold-Regulated Intrinsically Disordered Protein COR15A Open
Cold regulated protein 15A (COR15A) is a late embryogenesis abundant (LEA) protein from Arabidopsis thaliana which is expressed in response to low temperatures and dehydration. Here we use a combination of ion mobility-mass spectrometry (I…
View article: Triplet Quenching by Active Site Cysteine Residues Improves Photo-stability in Fatty Acid Photodecarboxylase
Triplet Quenching by Active Site Cysteine Residues Improves Photo-stability in Fatty Acid Photodecarboxylase Open
Enzyme photobiocatalysis uses light to drive high-energy transformations but is limited by the rarity of photoenzymes. Fatty acid photodecarboxylase (FAP), a recently discovered photoenzyme, enables fatty acid conversion to alkanes/alkenes…
View article: Self‐Assembly, Rearrangement, and Disassembly of {Cr <sub>6</sub> } Horseshoe Oligomers
Self‐Assembly, Rearrangement, and Disassembly of {Cr <sub>6</sub> } Horseshoe Oligomers Open
Molecular assemblies are commonly found in biological systems, and designing synthetic mimics of these is a challenge for modern chemistry. Here, we apply ion mobility mass spectrometry (IM‐MS), density functional theory (DFT), and mass‐se…
View article: Self‐Assembly, Rearrangement, and Disassembly of {Cr <sub>6</sub> } Horseshoe Oligomers
Self‐Assembly, Rearrangement, and Disassembly of {Cr <sub>6</sub> } Horseshoe Oligomers Open
Molecular assemblies are commonly found in biological systems, and designing synthetic mimics of these is a challenge for modern chemistry. Here, we apply ion mobility mass spectrometry (IM‐MS), density functional theory (DFT), and mass‐se…
View article: Hydrogen‐Deuterium Exchange Defines Ligand‐Induced Conformational Changes to the Class III Biotin Protein Ligase from <i>Saccharomyces cerevisiae</i>
Hydrogen‐Deuterium Exchange Defines Ligand‐Induced Conformational Changes to the Class III Biotin Protein Ligase from <i>Saccharomyces cerevisiae</i> Open
Biotin protein ligase (BPL) catalyzes the covalent attachment of biotin onto biotin‐dependent enzymes, where it functions as an essential cofactor. Eukaryotic BPLs are distinct due to the presence of a large N‐terminal extension to the con…
View article: Mapping the Conformational Landscape of the Cold-Regulated Intrinsically Disordered Protein COR15A
Mapping the Conformational Landscape of the Cold-Regulated Intrinsically Disordered Protein COR15A Open
Cold regulated protein 15A (COR15A) is a late embryogenesis abundant (LEA) protein from Arabidopsis thaliana which is expressed in response to low temperatures and dehydration. Here we use a combination of ion mobility-mass spectrometry (I…
View article: Tiny Droplets, Large Insights: Studying Peptide Aggregation with Droplet-based Microfluidics Hyphenated with Ion Mobility Mass Spectrometry
Tiny Droplets, Large Insights: Studying Peptide Aggregation with Droplet-based Microfluidics Hyphenated with Ion Mobility Mass Spectrometry Open
Peptide and protein aggregation is a hallmark of numerous neurodegenerative diseases. However, the structural characterization of oligomeric intermediates remains challenging due to their low abundance, heterogenic nature, and the complexi…
View article: Classification of Parkinson’s disease and isolated REM sleep behaviour disorder: delineating progression markers from the sebum volatilome
Classification of Parkinson’s disease and isolated REM sleep behaviour disorder: delineating progression markers from the sebum volatilome Open
Parkinson’s Disease (PD) has been associated with a distinct odour, emanating from the skin and strongest in sebum-rich areas. Here, volatile components from sebum were analysed directly with Thermal Desorption Gas Chromatography - Mass Sp…
View article: Trained dogs can detect the odor of Parkinson's disease
Trained dogs can detect the odor of Parkinson's disease Open
A definitive diagnostic test for Parkinson's disease (PD) remains elusive, so identification of potential biomarkers can facilitate diagnosis and early intervention. Two dogs were trained to distinguish between dry skin swabs obtained from…
View article: From Discovery to Delivery: A Rapid and Targeted Proteomics Workflow for Monitoring Chinese Hamster Ovary Biomanufacturing
From Discovery to Delivery: A Rapid and Targeted Proteomics Workflow for Monitoring Chinese Hamster Ovary Biomanufacturing Open
View article: Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry
Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry Open
View article: Twenty-Five years of High-throughput Screening of Biological Samples with Mass Spectrometry: Current Platforms and Emerging Methods
Twenty-Five years of High-throughput Screening of Biological Samples with Mass Spectrometry: Current Platforms and Emerging Methods Open
Robust high-throughput screening (HTS) approaches to discover new chemical entities are desirable for research and translation. Applications where high-throughput (HT) methods are particularly demanded also include the screening of potenti…
View article: Exploring the Increased Activity of the Blue Light-Dependent Photoenzyme Fatty Acid Photodecarboxylase under Violet Light
Exploring the Increased Activity of the Blue Light-Dependent Photoenzyme Fatty Acid Photodecarboxylase under Violet Light Open
Fatty acid photodecarboxylase (FAP) is a FAD-containing enzyme that catalyzes the light-driven decarboxylation of medium-long chain fatty acids. Over recent years, the substrate scope of FAP has been broadened to improve its potential to c…
View article: Site-selective photo-crosslinking for the characterisation of transient ubiquitin-like protein-protein interactions
Site-selective photo-crosslinking for the characterisation of transient ubiquitin-like protein-protein interactions Open
Non-covalent protein-protein interactions are one of the most fundamental building blocks in cellular signalling pathways. Despite this, they have been historically hard to identify using conventional methods due to their often weak and tr…
View article: Deciphering the safeguarding role of cysteine residues in p53 against H2O2-induced oxidation using high-resolution native mass spectrometry
Deciphering the safeguarding role of cysteine residues in p53 against H2O2-induced oxidation using high-resolution native mass spectrometry Open
The transcription factor p53 is exquisitely sensitive and selective to a broad variety of cellular environments. Several studies have reported that oxidative stress weakens the p53-DNA binding affinity for certain promoters depending on th…
View article: Measuring Size and Shape Heterogeneity in AAVs with Ion Mobility Mass Spectrometry
Measuring Size and Shape Heterogeneity in AAVs with Ion Mobility Mass Spectrometry Open
Adeno-associated viruses (AAVs) are at the forefront of biopharmaceutical development as gene therapy vectors. The successful approval of these medicines requires robust characterization including an assessment of therapeutic gene content.…
View article: Energetics of carboxylate-metal bonds in polymetallic rings
Energetics of carboxylate-metal bonds in polymetallic rings Open
Using collision-induced dissociation mass spectrometry and density functional theory, we assess the energetics of polymetallic [Cr 7 NiF 8 (O 2 CR) 16 ] − anions in vacuo , showing how the carboxylate influences the ring stability.
View article: Investigating the effects of circadian rhythm on the human skin lipidome
Investigating the effects of circadian rhythm on the human skin lipidome Open
The circadian rhythm is a 24 h cycle that harmonises the activity of organs – including the skin – to a daily routine using neurological and hormonal signals. We show that majority of sebum does not show this rhythm.
View article: Structural characterisation and dynamics of a paramagnetic {Cr <sub>12</sub> Ni <sub>3</sub> } seahorse in non-crystalline phases
Structural characterisation and dynamics of a paramagnetic {Cr <sub>12</sub> Ni <sub>3</sub> } seahorse in non-crystalline phases Open
A paramagnetic seahorse-like compound was characterised in non-crystalline phases. While the molecule retains its crystal structure in powder and solution, it is highly dynamic in the gas phase.
View article: The Interplay of Ion Mobility Mass Spectrometry and Computational Modelling for Structure Elucidation of Small Molecules: An Upper-Level Undergraduate Experiment
The Interplay of Ion Mobility Mass Spectrometry and Computational Modelling for Structure Elucidation of Small Molecules: An Upper-Level Undergraduate Experiment Open
A lab-based experiment, combining ion mobility mass spectrometry (IM-MS) with computational modelling techniques for the analysis of small molecules, has been developed for use in undergraduate teaching laboratories over eight years. Stude…
View article: Hybrid Mass Spectrometry Applied across the Production of Antibody Biotherapeutics
Hybrid Mass Spectrometry Applied across the Production of Antibody Biotherapeutics Open
Post expression from the host cells, biotherapeutics undergo downstream processing steps before final formulation. Mass spectrometry and biophysical characterization methods are valuable for examining conformational and stoichiometric chan…
View article: Structural Characterisation and Dynamics of a Paramagnetic {Cr12Ni3} Seahorse in Non-Crystalline Phases
Structural Characterisation and Dynamics of a Paramagnetic {Cr12Ni3} Seahorse in Non-Crystalline Phases Open
The complex [{Ni(cyclen)}2Cr12NiF18(O2CtBu)24] (where cyclen = 1,4,7,10-tetrazacyclododecane) crystallises as a fifteen-metal chain that is shaped like a seahorse. Given this is one of the longest finite, paramagnetic chains found, we were…
View article: From Discovery to Delivery: A Rapid and Targeted Proteomics Workflow for Monitoring Chinese Hamster Ovary Biomanufacturing
From Discovery to Delivery: A Rapid and Targeted Proteomics Workflow for Monitoring Chinese Hamster Ovary Biomanufacturing Open
Chinese hamster ovary (CHO) cells are the industrial workhorse for manufacturing biopharmaceuticals, including monoclonal antibodies. CHO cell line development requires a more data-driven approach for the accelerated identification of hype…
View article: Self-Assembly, Rearrangement and Disassembly of {Cr6} Horseshoe Oligomers
Self-Assembly, Rearrangement and Disassembly of {Cr6} Horseshoe Oligomers Open
Molecular assemblies are commonly found in biological systems; designing and reporting on synthetic mimics of these is a challenge for modern chemistry. Here, we apply ion mobility mass spectrometry (IM-MS), density functional theory calcu…
View article: Activation of L-histidine biosynthesis as a new antibiotic strategy against Mycobacterium tuberculosis
Activation of L-histidine biosynthesis as a new antibiotic strategy against Mycobacterium tuberculosis Open
The increasing prevalence of antimicrobial resistance is an important challenge that warrants new approaches to antibiotic development. Currently, all antibiotics inhibit biological processes. To explore whether activation of a biochemical…
View article: Nanohoops Favour Light‐Induced Energy Transfer over Charge Separation in Porphyrin/[10]CPP/Fullerene Rotaxanes
Nanohoops Favour Light‐Induced Energy Transfer over Charge Separation in Porphyrin/[10]CPP/Fullerene Rotaxanes Open
[2]Rotaxanes offer unique opportunities for studying and modulating charge separation and energy transfer, because the mechanical bond allows the robust, yet spatially dynamic tethering of photoactive groups. In this work, we synthesized […
View article: Nanohoops Favour Light‐Induced Energy Transfer over Charge Separation in Porphyrin/[10]CPP/Fullerene Rotaxanes
Nanohoops Favour Light‐Induced Energy Transfer over Charge Separation in Porphyrin/[10]CPP/Fullerene Rotaxanes Open
[2]Rotaxanes offer unique opportunities for studying and modulating charge separation and energy transfer, because the mechanical bond allows the robust, yet spatially dynamic tethering of photoactive groups. In this work, we synthesized […
View article: How storage post sampling influences the stability of sebum when used for mass spectrometry metabolomics analysis?
How storage post sampling influences the stability of sebum when used for mass spectrometry metabolomics analysis? Open
View article: Development and translation of a method of clinical utility for LC-MS/MS analysis to detect SARS-CoV-2 antigens from ONP swabs and saliva
Development and translation of a method of clinical utility for LC-MS/MS analysis to detect SARS-CoV-2 antigens from ONP swabs and saliva Open
During the COVID-19 pandemic, development of diagnostic tests was vital to chart the course and to reduce the impact of the infection. Continued testing and surveillance of vaccine escape will continue for years to come which presents an o…
View article: Combining Native Mass Spectrometry and Proteomics to Differentiate and Map the Metalloform Landscape in Metallothioneins
Combining Native Mass Spectrometry and Proteomics to Differentiate and Map the Metalloform Landscape in Metallothioneins Open
Within the intricate landscape of the proteome, approximately 30% of all proteins bind metal ions. This repertoire is even larger when considering all the different forms of a protein, known as proteoforms. Here, we propose the term "metal…