Peter A. Lund
YOU?
Author Swipe
View article: MmpL12 transports lipooligosaccharides and impacts virulence in Mycobacterium marinum
MmpL12 transports lipooligosaccharides and impacts virulence in Mycobacterium marinum Open
Lipooligosaccharides (LOSs) are polar glycolipids found in the cell envelope of many pathogenic mycobacteria. Here, we show that LOS transport in Mycobacterium marinum requires mmpL12 , a member of the resistance-nodulation-division family…
View article: Genome-wide screening using TraDIS accelerates identification of key adaptive mutations of longer-term evolution experiment in Escherichia coli
Genome-wide screening using TraDIS accelerates identification of key adaptive mutations of longer-term evolution experiment in Escherichia coli Open
Long term laboratory-based evolution experiments are a powerful tool that are increasingly being used to study fundamental aspects of evolution and to identify genes that contribute to overall fitness under different conditions. However, e…
View article: Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum
Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum Open
Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multidrug-resistant clones both alarmingly associated with high mortality. The molecular mechanisms underpinning these recalcitrant K. pn…
View article: Methods for studying microbial acid stress responses: from molecules to populations
Methods for studying microbial acid stress responses: from molecules to populations Open
The study of how micro-organisms detect and respond to different stresses has a long history of producing fundamental biological insights while being simultaneously of significance in many applied microbiological fields including infection…
View article: Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum
Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum Open
Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multi-drug resistant clones both alarmingly associated with high mortality. The molecular microbial genetics underpinning these recalcitr…
View article: Reviewer #3 (Public Review): Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum
Reviewer #3 (Public Review): Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum Open
Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multi-drug resistant clones both alarmingly associated with high mortality. The molecular microbial genetics underpinning these recalcitr…
View article: On the potential role of naturally occurring carboxylic organic acids as anti-infective agents: opportunities and challenges
On the potential role of naturally occurring carboxylic organic acids as anti-infective agents: opportunities and challenges Open
Carboxylic organic acids are intermediates of central carbon metabolic pathways (e.g. acetic, propionic, citric, and lactic acid) long known to have potent antimicrobial potential, mainly at acidic pHs. The food industry has been leveragin…
View article: Exploitation of microbial activities at low pH to enhance planetary health
Exploitation of microbial activities at low pH to enhance planetary health Open
Awareness is growing that human health cannot be considered in isolation but is inextricably woven with the health of the environment in which we live. It is, however, under-recognized that the sustainability of human activities strongly r…
View article: Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum
Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum Open
Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multidrug-resistant clones both alarmingly associated with high mortality. The molecular mechanisms underpinning these recalcitrant K. pn…
View article: Mycobacterial chaperonins in cellular proteostasis: Evidence for chaperone function of Cpn60.1 and Cpn60.2‐mediated protein folding
Mycobacterial chaperonins in cellular proteostasis: Evidence for chaperone function of Cpn60.1 and Cpn60.2‐mediated protein folding Open
Mycobacterium tuberculosis encodes two chaperonin proteins, MtbCpn60.1 and MtbCpn60.2, that share substantial sequence similarity with the Escherichia coli chaperonin, GroEL. However, unlike GroEL, MtbCpn60.1 and MtbCpn60.2 purify as lower…
View article: Transposon mutagenesis screen in <i>Klebsiella pneumoniae</i> identifies genetic determinants required for growth in human urine and serum
Transposon mutagenesis screen in <i>Klebsiella pneumoniae</i> identifies genetic determinants required for growth in human urine and serum Open
Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multi-drug resistant clones both alarmingly associated with high mortality. The molecular microbial genetics underpinning these recalcitr…
View article: Editorial: Microbial Stress: From Model Organisms to Applications in Food, Microbiotechnology and Medicine
Editorial: Microbial Stress: From Model Organisms to Applications in Food, Microbiotechnology and Medicine Open
EDITORIAL article Front. Microbiol., 27 June 2022Sec. Microbial Physiology and Metabolism https://doi.org/10.3389/fmicb.2022.945573
View article: Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey on E. coli K-12
Use of Transposon Directed Insertion-Site Sequencing to Probe the Antibacterial Mechanism of a Model Honey on E. coli K-12 Open
Antimicrobial resistance is an ever-growing health concern worldwide that has created renewed interest in the use of traditional anti-microbial treatments, including honey. However, understanding the underlying mechanism of the anti-microb…
View article: Chaperonin Abundance Enhances Bacterial Fitness
Chaperonin Abundance Enhances Bacterial Fitness Open
The ability of chaperonins to buffer mutations that affect protein folding pathways suggests that their abundance should be evolutionarily advantageous. Here, we investigate the effect of chaperonin overproduction on cellular fitness in Es…
View article: Mapping the Transcriptional and Fitness Landscapes of a Pathogenic E. coli Strain: The Effects of Organic Acid Stress under Aerobic and Anaerobic Conditions
Mapping the Transcriptional and Fitness Landscapes of a Pathogenic E. coli Strain: The Effects of Organic Acid Stress under Aerobic and Anaerobic Conditions Open
Several methods are available to probe cellular responses to external stresses at the whole genome level. RNAseq can be used to measure changes in expression of all genes following exposure to stress, but gives no information about the con…
View article: A Bayesian non-parametric mixed-effects model of microbial growth curves
A Bayesian non-parametric mixed-effects model of microbial growth curves Open
Substantive changes in gene expression, metabolism, and the proteome are manifested in overall changes in microbial population growth. Quantifying how microbes grow is therefore fundamental to areas such as genetics, bioengineering, and fo…
View article: Understanding How Microorganisms Respond to Acid pH Is Central to Their Control and Successful Exploitation
Understanding How Microorganisms Respond to Acid pH Is Central to Their Control and Successful Exploitation Open
Microbes from the three domains of life, Bacteria, Archaea, and Eukarya, share the need to sense and respond to changes in the external and internal concentrations of protons. When the proton concentration is high, acidic conditions prevai…
View article: The Signaling Molecule Indole Inhibits Induction of the AR2 Acid Resistance System in Escherichia coli
The Signaling Molecule Indole Inhibits Induction of the AR2 Acid Resistance System in Escherichia coli Open
Induction of the AR2 acid response system of Escherichia coli occurs at a moderately low pH (pH 5.5) and leads to high levels of resistance to pH levels below 2.5 in the presence of glutamate. Induction is mediated in part by the EvgAS two…
View article: Chaperonin Abundance Boosts Bacterial Fitness
Chaperonin Abundance Boosts Bacterial Fitness Open
The ability of chaperonins to buffer mutations that affect protein folding pathways suggests that their abundance should be evolutionarily advantageous. Here, we investigate the effect of chaperonin overproduction on cellular fitness in Es…
View article: Transposon Directed Insertion-site Sequencing of a BW25113 ascA (yecA) deletion mutant
Transposon Directed Insertion-site Sequencing of a BW25113 ascA (yecA) deletion mutant Open
We carried out saturated transposon mutagenesis of an ascA deletion mutant, determined the location of the transposon insertions using Illumina sequencing and mapped them to the BW25113 reference genome (CP009273.1). We have deposited thre…
View article: A Bayesian Non-parametric Mixed-Effects Model of Microbial Phenotypes
A Bayesian Non-parametric Mixed-Effects Model of Microbial Phenotypes Open
Substantive changes in gene expression, metabolism, and the proteome are manifested in overall changes in microbial population growth. Quantifying how microbes grow is therefore fundamental to areas such as genetics, bioengineering, and fo…
View article: Molecular approaches to understand the effect of acetic acid in uropathogenic E. coli
Molecular approaches to understand the effect of acetic acid in uropathogenic E. coli Open
Acetic acid has long been known for its antibacterial activity. We are using TraDIS to investigate the molecular mechanisms by which acetic acid acts as an antibacterial agent. To do this, we grew a high-density transposon library in uropa…
View article: High throughput approaches to study laboratory-based evolution of E. coli for enhanced growth at low pH
High throughput approaches to study laboratory-based evolution of E. coli for enhanced growth at low pH Open
Laboratory-based evolution has become a widely used method to explore fundamental questions about evolution as a process, and is also a powerful tool to study the link between genotype and phenotype. We have evolved six populations of E. c…
View article: Use of cellular and high throughput genetic approaches to unravel the antibacterial mechanism of honey
Use of cellular and high throughput genetic approaches to unravel the antibacterial mechanism of honey Open
The medical importance of honey has been extensively demonstrated. Although high osmolarity, acidity and hydrogen peroxide (H2O2) proved to be the most prevalent factors in honey’s activity, the underlying antimicrobial mechanism remains o…
View article: Synergistic Impacts of Organic Acids and pH on Growth of Pseudomonas aeruginosa: A Comparison of Parametric and Bayesian Non-parametric Methods to Model Growth
Synergistic Impacts of Organic Acids and pH on Growth of Pseudomonas aeruginosa: A Comparison of Parametric and Bayesian Non-parametric Methods to Model Growth Open
Different weak organic acids have significant potential as topical treatments for wounds infected by opportunistic pathogens that are recalcitrant to standard treatments. These acids have long been used as bacteriostatic compounds in the f…