Peter Askjaer
YOU?
Author Swipe
View article: Nuclear lamina-associated domain biogenesis is regulated by nuclear pore density during embryogenesis and mediates UV protection
Nuclear lamina-associated domain biogenesis is regulated by nuclear pore density during embryogenesis and mediates UV protection Open
SUMMARY Lamina-associated domains (LADs) are critical for genome organization and function, but their formation during development is not well understood. Here, we use DamID and image analysis to reveal the dynamics of LAD biogenesis durin…
View article: The transsulfuration pathway suppresses the embryonic lethal phenotype of glutathione reductase mutants in <i>Caenorhabditis elegans</i>
The transsulfuration pathway suppresses the embryonic lethal phenotype of glutathione reductase mutants in <i>Caenorhabditis elegans</i> Open
The gsr-1 gene encodes the only glutathione reductase in Caenorhabditis elegans and gsr-1 loss-of-function alleles have a fully penetrant embryonic lethal phenotype. Therefore, maintenance of glutathione redox homeostasis is essential for …
View article: An interkinetic envelope surrounds chromosomes between meiosis I and II in <i>C. elegans</i> oocytes
An interkinetic envelope surrounds chromosomes between meiosis I and II in <i>C. elegans</i> oocytes Open
At the end of cell division, the nuclear envelope reassembles around the decondensing chromosomes. Female meiosis culminates in two consecutive cell divisions of the oocyte, meiosis I and II, which are separated by a brief transition phase…
View article: An interkinetic envelope surrounds chromosomes between meiosis I and II in C. elegans oocytes
An interkinetic envelope surrounds chromosomes between meiosis I and II in C. elegans oocytes Open
At the end of cell division, the nuclear envelope reassembles around the decondensing chromosomes. Female meiosis culminates in two consecutive cell divisions of the oocyte, meiosis I and II, which are separated by a brief transition phase…
View article: A human progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in C. elegans
A human progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in C. elegans Open
Alterations in the nuclear envelope are linked to a variety of rare diseases termed laminopathies. A single amino acid substitution at position 12 (A12T) of the human nuclear envelope protein BAF (Barrier to Autointegration Factor) causes …
View article: A progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in <i>C. elegans</i>
A progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in <i>C. elegans</i> Open
Alterations in the nuclear envelope are linked to a variety of rare diseases termed laminopathies. These include both tissue specific and systemic diseases. A single amino acid substitution in human barrier to autointegration factor (BAF) …
View article: Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in <i>C. elegans</i> embryos
Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in <i>C. elegans</i> embryos Open
The nuclear envelope, which protects and organizes the genome, is dismantled during mitosis. In the Caenorhabditis elegans zygote, nuclear envelope breakdown (NEBD) of the parental pronuclei is spatially and temporally regulated during mit…
View article: Nucleoporin foci are stress‐sensitive condensates dispensable for <i>C. elegans</i> nuclear pore assembly
Nucleoporin foci are stress‐sensitive condensates dispensable for <i>C. elegans</i> nuclear pore assembly Open
Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier between nucleoplasm and cytoplasm. Nucleoporins also localize in cytoplasmic foci proposed to function as pore pre‐assembly intermediates. Here, we characterize …
View article: Tissue-specific chromatin-binding patterns of <i>Caenorhabditis elegans</i> heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression
Tissue-specific chromatin-binding patterns of <i>Caenorhabditis elegans</i> heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression Open
Heterochromatin is characterized by an enrichment of repetitive elements and low gene density and is often maintained in a repressed state across cell division and differentiation. The silencing is mainly regulated by repressive histone ma…
View article: Mechanisms of Nuclear Pore Complex disassembly by the mitotic Polo-Like Kinase 1 (PLK-1) in<i>C. elegans</i>embryos
Mechanisms of Nuclear Pore Complex disassembly by the mitotic Polo-Like Kinase 1 (PLK-1) in<i>C. elegans</i>embryos Open
The nuclear envelope, which protects and organizes the interphase genome, is dismantled during mitosis. In the C. elegans zygote, nuclear envelope breakdown (NEBD) of the parental pronuclei is spatially and temporally regulated during mito…
View article: Tissue-specific chromatin binding patterns of<i>C. elegans</i>heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression
Tissue-specific chromatin binding patterns of<i>C. elegans</i>heterochromatin proteins HPL-1 and HPL-2 reveal differential roles in the regulation of gene expression Open
Heterochromatin is characterized by an enrichment of repetitive elements and low gene density and is often maintained in a repressed state across cell division and differentiation. The silencing is mainly regulated by repressive histone ma…
View article: Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>Caenorhabditis elegans</i>
Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>Caenorhabditis elegans</i> Open
Control of gene expression in specific tissues and/or at certain stages of development allows the study and manipulation of gene function with high precision. Site-specific genome recombination by the flippase (FLP) and cyclization recombi…
View article: A multiparametric anti-aging CRISPR screen uncovers a role for BAF in protein translation
A multiparametric anti-aging CRISPR screen uncovers a role for BAF in protein translation Open
Progeria syndromes are very rare, incurable premature aging conditions recapitulating most aging features. Here, we report the first whole genome, multiparametric CRISPR anti-aging screen, identifying 43 new genes that can reverse multiple…
View article: Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans
Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Open
Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier that separates nucleoplasm from cytoplasm. Nups have also been observed in cytoplasmic foci proposed to function as pore pre-assembly intermediates. Here we char…
View article: Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>C. elegans</i>
Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>C. elegans</i> Open
Control of gene expression in specific tissues and/or at certain stages of development allows the study and manipulation of gene function with high precision. Site-specific genome recombination by the Flippase (FLP) and Cre enzymes has pro…
View article: THSC/TREX-2 deficiency causes replication stress and genome instability in <i>Caenorhabditis elegans</i>
THSC/TREX-2 deficiency causes replication stress and genome instability in <i>Caenorhabditis elegans</i> Open
Transcription is an essential process of DNA metabolism, yet it makes DNA more susceptible to DNA damage. THSC/TREX-2 is a conserved eukaryotic protein complex with a key role in mRNP biogenesis and maturation that prevents genome instabil…
View article: DamID identifies targets of CEH-60/PBX that are associated with neuron development and muscle structure in Caenorhabditis elegans
DamID identifies targets of CEH-60/PBX that are associated with neuron development and muscle structure in Caenorhabditis elegans Open
Transcription factors govern many of the time- and tissue-specific gene expression events in living organisms. CEH-60, a homolog of the TALE transcription factor PBX in vertebrates, was recently characterized as a new regulator of intestin…
View article: Genome-wide analysis links emerin to neuromuscular junction activity in Caenorhabditis elegans
Genome-wide analysis links emerin to neuromuscular junction activity in Caenorhabditis elegans Open
BackgroundLaminopathies are diseases characterized by defects in nuclear envelope structure. A well-known example is Emery-Dreifuss muscular dystrophy, which is caused by mutations in the human lamin A/C and emerin genes. While most nuclea…
View article: Loss of an H3K9me anchor rescues laminopathy-linked changes in nuclear organization and muscle function in an Emery-Dreifuss muscular dystrophy model
Loss of an H3K9me anchor rescues laminopathy-linked changes in nuclear organization and muscle function in an Emery-Dreifuss muscular dystrophy model Open
Mutations in the nuclear structural protein lamin A produce rare, tissue-specific diseases called laminopathies. The introduction of a human Emery-Dreifuss muscular dystrophy (EDMD)-inducing mutation into the C. elegans lamin (LMN-Y59C), r…
View article: CEH-60/PBX regulates vitellogenesis and cuticle permeability through intestinal interaction with UNC-62/MEIS in Caenorhabditis elegans
CEH-60/PBX regulates vitellogenesis and cuticle permeability through intestinal interaction with UNC-62/MEIS in Caenorhabditis elegans Open
The onset of sexual maturity involves dramatic changes in physiology and gene expression in many animals. These include abundant yolk protein production in egg-laying species, an energetically costly process under extensive transcriptional…
View article: Nuclear Organization in Stress and Aging
Nuclear Organization in Stress and Aging Open
The eukaryotic nucleus controls most cellular processes. It is isolated from the cytoplasm by the nuclear envelope, which plays a prominent role in the structural organization of the cell, including nucleocytoplasmic communication, chromat…
View article: Spatiotemporal control of gene expression to analyse nuclear organisation and beyond
Spatiotemporal control of gene expression to analyse nuclear organisation and beyond Open
Resumen del trabajo presentado en el VII Spanish Worm Meeting, celebrado en Barcelona (España), los días 28 y 29 de marzo de 2019
View article: Loss of glutathione redox homeostasis impairs proteostasis by inhibiting autophagy-dependent protein degradation
Loss of glutathione redox homeostasis impairs proteostasis by inhibiting autophagy-dependent protein degradation Open
In the presence of aggregation-prone proteins, the cytosol and endoplasmic reticulum (ER) undergo a dramatic shift in their respective redox status, with the cytosol becoming more oxidized and the ER more reducing. However, whether and how…