Peter F. Bonventre
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View article: Crystal structure of a biologically inactive mutant of toxic shock syndrome toxin‐1 at 2.5 A resolution
Crystal structure of a biologically inactive mutant of toxic shock syndrome toxin‐1 at 2.5 A resolution Open
Toxic shock syndrome toxin‐1 (TSST‐1) is one of a family of staphylococcal exotoxins recognized as microbial superantigens. The toxin plays a dominant role in the genesis of toxic shock in humans through a massive activation of the immune …
View article: A toxic shock syndrome toxin 1 mutant that defines a functional site critical for T-cell activation
A toxic shock syndrome toxin 1 mutant that defines a functional site critical for T-cell activation Open
Toxic shock syndrome toxin 1 (TSST-1), a superantigen produced by Staphylococcus aureus, is a causative agent of toxic shock syndrome (TSS). This superantigen is a potent stimulator of T cells and macrophages/monocytes, resulting in the re…
View article: Biological activity of toxic shock syndrome toxin 1 and a site-directed mutant, H135A, in a lipopolysaccharide-potentiated mouse lethality model
Biological activity of toxic shock syndrome toxin 1 and a site-directed mutant, H135A, in a lipopolysaccharide-potentiated mouse lethality model Open
A recombinant of toxic shock syndrome toxin 1 (TSST-1) which contains a single histidine-to-alanine mutation at residue 135 (H135A) was analyzed for toxicity and vaccine potential in a lipopolysaccharide (LPS)-potentiated mouse lethality m…
View article: Role of a carboxy-terminal site of toxic shock syndrome toxin 1 in eliciting immune responses of human peripheral blood mononuclear cells
Role of a carboxy-terminal site of toxic shock syndrome toxin 1 in eliciting immune responses of human peripheral blood mononuclear cells Open
Staphylococcus aureus toxic shock syndrome toxin 1 (TSST-1) is involved in the pathogenesis of toxic shock syndrome and perhaps other staphylococcal diseases. Recently, the C-terminal part of the TSST-1 toxin has been shown to be responsib…
View article: A mutation at histidine residue 135 of toxic shock syndrome toxin yields an immunogenic protein with minimal toxicity
A mutation at histidine residue 135 of toxic shock syndrome toxin yields an immunogenic protein with minimal toxicity Open
Structure-function studies have revealed that the region between amino acids 115 and 141 of toxic shock syndrome toxin 1 (TSST-1) constitutes a biologically active domain. A critical residue appears to be histidine 135, since a site-direct…
View article: Toxicity of recombinant toxic shock syndrome toxin 1 and mutant toxins produced by Staphylococcus aureus in a rabbit infection model of toxic shock syndrome
Toxicity of recombinant toxic shock syndrome toxin 1 and mutant toxins produced by Staphylococcus aureus in a rabbit infection model of toxic shock syndrome Open
Menstrually associated toxic shock syndrome (TSS) is attributed primarily to the effects of staphylococcal exotoxin toxic shock syndrome toxin 1 (TSST-1). A region of the 194-amino-acid toxin spanning residues 115 through 144 constitutes a…
View article: Cyclosporin A treatment converts Leishmania donovani-infected C57BL/10 (curing) mice to a noncuring phenotype
Cyclosporin A treatment converts Leishmania donovani-infected C57BL/10 (curing) mice to a noncuring phenotype Open
Cyclosporin A prevents visceralization of Leishmania major infection of BALB/c mice (N. C. Behforouz, C. D. Wenger, and B. A. Mathison, J. Immunol. 136:3067-3075, 1986; W. Solbach, K. Forberg, E. Kammerer, C. Bogdan, and M. Rollinghoff, J.…
View article: Mutants of staphylococcal toxic shock syndrome toxin 1: mitogenicity and recognition by a neutralizing monoclonal antibody
Mutants of staphylococcal toxic shock syndrome toxin 1: mitogenicity and recognition by a neutralizing monoclonal antibody Open
Toxic shock syndrome toxin 1 (TSST-1), a 22-kilodalton protein made by strains of Staphylococcus aureus harboring the chromosomal toxin gene, may elicit toxic shock syndrome in humans. In vitro, TSST-1 induces T cells to proliferate and ma…
View article: Protection of rabbits in an infection model of toxic shock syndrome (TSS) by a TSS toxin-1-specific monoclonal antibody
Protection of rabbits in an infection model of toxic shock syndrome (TSS) by a TSS toxin-1-specific monoclonal antibody Open
An anti-TSST-1-specific monoclonal antibody (MAb 8-5-7) was tested for its protective capacity in a rabbit infection model to toxic shock syndrome (TSS). The challenge strain of Staphylococcus aureus (RN4710), which contained a plasmid enc…
View article: Neutralization of toxic shock syndrome toxin-1 by monoclonal antibodies in vitro and in vivo
Neutralization of toxic shock syndrome toxin-1 by monoclonal antibodies in vitro and in vivo Open
Sixteen monoclonal antibodies (MAbs) directed against toxic shock syndrome toxin-1 (TSST-1) were generated by immunization of mice with purified TSST-1 and subsequent fusion of spleen cells with myeloma cells. Antibody-producing clones, id…
View article: Synergistic induction of interleukin-1 by endotoxin and toxic shock syndrome toxin-1 using rat macrophages
Synergistic induction of interleukin-1 by endotoxin and toxic shock syndrome toxin-1 using rat macrophages Open
We studied interleukin-1 (IL-1) secretion by rat peritoneal exudate macrophages stimulated with purified toxic shock syndrome toxin-1 (TSST-1). TSST-1 was observed to be a more potent inducer of IL-1 than was endotoxin. The induction of IL…
View article: Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation
Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation Open
To express toxicity in living cells, diphtheria toxin (DT) must cross a membrane barrier and reach its target in the cytosol. Here we examine the entry of DT into the toxin-sensitive monkey kidney (Vero) cells. Using electron microscopy we…
View article: Visceral leishmaniasis in congenic mice of susceptible and resistant phenotypes: T-lymphocyte-mediated immunosuppression
Visceral leishmaniasis in congenic mice of susceptible and resistant phenotypes: T-lymphocyte-mediated immunosuppression Open
This paper continues a comparative study (A. D. Nickol and P. F. Bonventre, Infect. Immun. 50:160-168, 1985) describing immune responses exhibited by congenic, Lshs mouse strains C57B1/10 (cure) and B10.D2 (noncure) during the course of di…
View article: Visceral leishmaniasis in congenic mice of susceptible and resistant phenotypes: immunosuppression by adherent spleen cells
Visceral leishmaniasis in congenic mice of susceptible and resistant phenotypes: immunosuppression by adherent spleen cells Open
Visceral leishmaniasis is one of several parasitic diseases of humans characterized by immune suppression. A murine model of disseminated leishmaniasis utilizing inbred strains of specific genetic constitution was used to study the mechani…
View article: Synergistic effect of glucantime and a liposome-encapsulated muramyl dipeptide analog in therapy of experimental visceral leishmaniasis
Synergistic effect of glucantime and a liposome-encapsulated muramyl dipeptide analog in therapy of experimental visceral leishmaniasis Open
A regimen of immunostimulation with 6-0-stearoyl-N-acetylmuramyl-L-alpha-aminobutyryl-D-isoglutamine, a lipophilic analog of muramyl dipeptide, combined with antimonial drug therapy was evaluated in the treatment of visceral leishmaniasis …
View article: Evidence for pseudomonas exotoxin A receptors on plasma membrane of toxin-sensitive lm fibroblasts
Evidence for pseudomonas exotoxin A receptors on plasma membrane of toxin-sensitive lm fibroblasts Open
Pseudomonas exotoxin A enters mouse LM fibroblasts by receptor-mediated endocytosis and ultimately causes cell death. Here we present evidence for the existence of a specific receptor for the toxin. Toxin association with LM cells at 18 an…
View article: Rapid screening assay for toxic shock syndrome toxin production by Staphylococcus aureus
Rapid screening assay for toxic shock syndrome toxin production by Staphylococcus aureus Open
A rapid immunoblot assay (TST-blot) was developed and used to screen Staphylococcus aureus isolates for toxic shock syndrome toxin (TST) production. Growth from an 18-h stab inoculum of S. aureus on brain heart infusion agar was transferre…
View article: Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources
Production of staphylococcal enterotoxin F and pyrogenic exotoxin C by Staphylococcus aureus isolates from toxic shock syndrome-associated sources Open
A total of 136 isolates of Staphylococcus aureus were tested for production of staphylococcal enterotoxin F (SEF) and pyrogenic exotoxin C (PEC), both of which have been identified as reliable indicators of toxic shock syndrome (TSS)-assoc…
View article: A role for oxygen-dependent mechanisms in killing of <i>Leishmania donovani</i> tissue forms by activated macrophages.
A role for oxygen-dependent mechanisms in killing of <i>Leishmania donovani</i> tissue forms by activated macrophages. Open
Leishmania donovani, the causative agent of visceral leishmaniasis, infects macrophages (M phi ) of susceptible vertebrates. Immunologically activated M phi are leishmanicidal, but the mechanisms involved in the killing process are not wel…
View article: Specific receptor binding of staphylococcal enterotoxins by murine splenic lymphocytes
Specific receptor binding of staphylococcal enterotoxins by murine splenic lymphocytes Open
We describe a reliable assay to measure the specific binding of 125I-labeled staphylococcal enterotoxin A (SEA) by murine spleen cells. Toxin binding by lymphocytes was specific in that it was inhibited by unlabeled SEA but not by unrelate…
View article: Elimination of Leishmania donovani amastigotes by activated macrophages
Elimination of Leishmania donovani amastigotes by activated macrophages Open
Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor ce…
View article: Staphylococcal enterotoxins fail to disrupt membrane integrity or synthetic functions of Henle 407 intestinal cells
Staphylococcal enterotoxins fail to disrupt membrane integrity or synthetic functions of Henle 407 intestinal cells Open
The potential cytotoxic activity of purified staphylococcal enterotoxins for mammalian cells was evaluated. The effects of staphylococcal enterotoxins A (SEA) and B (SEB) on cell membrane integrity as measured by leakage of labeled cytopla…
View article: Shigella Infection of Henle Intestinal Epithelial Cells: Role of the Host Cell
Shigella Infection of Henle Intestinal Epithelial Cells: Role of the Host Cell Open
The process of Henle 407 embryonic intestinal epithelial cell infection by Shigella flexneri 2a M42-43 was studied in an in vitro model system. The role of the Henle cell was assessed. It was established that entry of S. flexneri into cell…
View article: Shigella infection of Henle intestinal epithelial cells: role of the bacterium
Shigella infection of Henle intestinal epithelial cells: role of the bacterium Open
Epithelial cell infection by Shigella flexneri 2a was studied in an in vitro model system. Using the Henle 407 human intestinal epithelial cell line as host cells, a standardized experimental protocol which allowed quantitative measurement…
View article: Killing of Intraphagocytic <i>Staphylococcus aureus</i> by Dihydrostreptomycin Entrapped Within Liposomes
Killing of Intraphagocytic <i>Staphylococcus aureus</i> by Dihydrostreptomycin Entrapped Within Liposomes Open
Liposomes (phospholipid vesicles) may serve as vehicles of antibiotic transport to intracellular sites. Liposomes containing entrapped dihydrostreptomycin enhanced killing of Staphylococcus aureus contained within phagocytic vacuoles.
View article: Anomalous high native resistance to athymic mice to bacterial pathogens
Anomalous high native resistance to athymic mice to bacterial pathogens Open
Congenitally athymic (nude) mice exhibited an anomalous high resistance against infections with the facultative intracellular parasite Listeria monocytogenes and other bacterial pathogens. Protection against lethal infection was demonstrat…