Explanipedia

Extension of lifespan by epicatechin, halofuginone and mitoglitazone in male but not female genetically heterogeneous mice Open

Randy Strong, James F. Nelson, Molly A. Bogue, Jerry R. Colca, Martin S. Denzel , et al. · 2025

Mice bred in 2021 were tested by the Interventions Testing Program (ITP) for possible lifespan benefits of 2BAct (2BA), dichloroacetate (DCA), Epicatechin (EPI), Forskolin (FSK), Halofuginone (HAL) and Mitoglitazone (MIT). All agents were …

End-of-life pathology in UM-HET3 mice treated with 16 α‑hydroxyestradiol or late‑start canagliflozin Open

Jessica M. Snyder, David Harrison, Ron Korstanje, Brett C. Ginsburg, Peter C. Reifsnyder , et al. · 2025

Canagliflozin (Cana) started at 16 months of age and 16-hydroxy-estradiol (OH_Est) started at 12 months each led to significant increases in lifespan in male UM-HET3 mice but significant decreases in female lifespan. To seek insights into …

Correction to: Lifespan effects in male UM‑HET3 mice treated with sodium thiosulfate, 16-hydroxyestradiol, and late‑start canagliflozin Open

Richard A. Miller, David Harrison, Gino Cortopassi, Ishmael Dehghan, Elizabeth Fernández , et al. · 2025

Analysis of lifespan across diversity outbred mouse studies identifies multiple longevity-associated loci Open

Martin N. Mullis, Kevin M. Wright, Anil Raj, Daniel M. Gatti, Peter C. Reifsnyder , et al. · 2025

Lifespan is an integrative phenotype whose genetic architecture is likely to highlight multiple processes with high impact on health and aging. Here, we conducted a genetic mega-analysis of longevity in Diversity Outbred (DO) mice that inc…

Age associated changes in behavioral and memory functions in genetically heterogeneous mice UM‐HET3 Open

Harpreet Kaur, Bretton Badenoch, Peter C. Reifsnyder, Kristen M.S. O’Connell, Richard A. Miller , et al. · 2024

Background Previous studies have documented age‐related changes in behavior and cognitive functions and investigated the molecular changes in aging brain using inbred mouse strains such as C57BL/6, BALB/c etc. In this study using a genetic…

Analysis of lifespan across Diversity Outbred mouse studies identifies multiple longevity-associated loci Open

Martin N. Mullis, Kevin M. Wright, Anil Raj, Daniel M. Gatti, Peter C. Reifsnyder , et al. · 2024

Lifespan is an integrative phenotype whose genetic architecture is likely to highlight multiple processes with high impact on health and aging. Here, we conduct a genetic meta-analysis of longevity in Diversity Outbred (DO) mice that inclu…

Lifespan effects in male UM-HET3 mice treated with sodium thiosulfate, 16-hydroxyestriol, and late-start canagliflozin Open

Richard A. Miller, David Harrison, Gino Cortopassi, Ishmael Dehghan, Elizabeth Fernández , et al. · 2024

Contributions of mouse genetic strain background to age-related phenotypes in physically active HET3 mice Open

Jake W. Willows, Zahra Alshahal, Naeemah M. Story, Michele Joana Alves, Pablo Vidal , et al. · 2024

Astaxanthin and meclizine extend lifespan in UM-HET3 male mice; fisetin, SG1002 (hydrogen sulfide donor), dimethyl fumarate, mycophenolic acid, and 4-phenylbutyrate do not significantly affect lifespan in either sex at the doses and schedules used Open

David Harrison, Randy Strong, Peter C. Reifsnyder, Nadia Rosenthal, Ron Korstanje , et al. · 2023

In genetically heterogeneous (UM-HET3) mice produced by the CByB6F1 × C3D2F1 cross, the Nrf2 activator astaxanthin (Asta) extended the median male lifespan by 12% ( p = 0.003, log-rank test), while meclizine (Mec), an mTORC1 inhibitor, ext…

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Jake W. Willows, Morganne Robinson, Zahra Alshahal, Samantha K. Morrison, Gargi Mishra , et al. · 2023

Cover legend: The cover image is based on the Research Article Age‐related changes to adipose tissue and peripheral neuropathy in genetically diverse HET3 mice differ by sex and are not mitigated by rapamycin longevity treatment by Jake W.…

Age‐related changes to adipose tissue and peripheral neuropathy in genetically diverse <span>HET3</span> mice differ by sex and are not mitigated by rapamycin longevity treatment Open

Jake W. Willows, Morganne Robinson, Zahra Alshahal, Samantha K. Morrison, Gargi Mishra , et al. · 2023

Neural communication between the brain and adipose tissues regulates energy expenditure and metabolism through modulation of adipose tissue functions. We have recently demonstrated that under pathophysiological conditions (obesity, diabete…

Lifespan benefits for the combination of rapamycin plus acarbose and for captopril in genetically heterogeneous mice Open

Randy Strong, Richard A. Miller, Catherine Cheng, James F. Nelson, Jonathan Gelfond , et al. · 2022

Mice bred in 2017 and entered into the C2017 cohort were tested for possible lifespan benefits of (R/S)‐1,3‐butanediol (BD), captopril (Capt), leucine (Leu), the Nrf2‐activating botanical mixture PB125, sulindac, syringaresinol, or the com…

Rapamycin/metformin co‐treatment normalizes insulin sensitivity and reduces complications of metabolic syndrome in type 2 diabetic mice Open

Peter C. Reifsnyder, Kevin Flurkey, Rosalinda Doty, Nigel A. Calcutt, Robert A. Koza , et al. · 2022

Rapamycin treatment has positive and negative effects on progression of type 2 diabetes (T2D) in a recombinant inbred polygenic mouse model, male NONcNZO10/LtJ (NcZ10). Here, we show that combination treatment with metformin ameliorates ne…

17‐a‐estradiol late in life extends lifespan in aging UM‐HET3 male mice; nicotinamide riboside and three other drugs do not affect lifespan in either sex Open

David E. Harrison, Randy Strong, Peter C. Reifsnyder, Navasuja Kumar, Elizabeth Fernández , et al. · 2021

In genetically heterogeneous mice produced by the CByB6F1 x C3D2F1 cross, the “non‐feminizing” estrogen, 17‐α‐estradiol (17aE2), extended median male lifespan by 19% ( p < 0.0001, log‐rank test) and 11% ( p = 0.007) when fed at 14.4 ppm st…

Rapamycin‐mediated mouse lifespan extension: Late‐life dosage regimes with sex‐specific effects Open

Randy Strong, Richard A. Miller, Molly A. Bogue, Elizabeth Fernández, Martin A. Javors , et al. · 2020

To see if variations in timing of rapamycin (Rapa), administered to middle aged mice starting at 20 months, would lead to different survival outcomes, we compared three dosing regimens. Initiation of Rapa at 42 ppm increased survival signi…

Canagliflozin extends life span in genetically heterogeneous male but not female mice Open

Richard A. Miller, David E. Harrison, David B. Allison, Molly A. Bogue, Lucas Kniess Debarba , et al. · 2020

Canagliflozin (Cana) is an inhibitor of the sodium glucose transporter 2 (SGLT2), and is thought to act by blocking renal reuptake and intestinal absorption of glucose. Cana is FDA-approved for treatment of diabetes, and affords protection…

Canagliflozin Extends Lifespan in Genetically Heterogeneous Male But Not Female Mice Open

Richard A. Miller, David E. Harrison, David B. Allison, Molly A. Bogue, Vivian Diaz , et al. · 2020

Canagliflozin (Cana) is an inhibitor of the sodium glucose transporter 2 (SGLT2), and is thought to act by blocking renal reuptake and intestinal absorption of glucose. Cana is FDA-approved for treatment of diabetes, and affords protection…

Differential Effects of Rapamycin on Glucose Metabolism in Nine Inbred Strains Open

Peter C. Reifsnyder, Austen Te, David E. Harrison · 2019

Studies in mice suggest that rapamycin has a negative impact on glucose homeostasis by inducing insulin resistance. However, results have been inconsistent and difficult to assess because the strains, methods of treatment, and analysis var…

Protocol for Albuwell M kit: Murine Microalbuminuria ELISA By Exocell Inc v1 Open

Kathi Burke, Peter C. Reifsnyder · 2019

Summary: Albuwell M is an indirect competitive ELISA designed to monitor kidney function in the mouse by measurement of urinary albumin. To complete the assay, sample and rabbit anti-murine albumin antibody are added to albumin coated well…

Creatinine Companion Protocol Assay (Exocell) v1 Open

Kathi Burke, Peter C. Reifsnyder · 2019

Summary: This assay is designed for companion use with Exocell’s immunospecific ELISAs for urine albumin excretion, allowing expression of results as µg albumin per mg creatinine in the urine. Adaptation of creatinine measurement to microt…

Rapamycin treatment benefits glucose metabolism in mouse models of type 2 diabetes Open

Peter C. Reifsnyder, Kevin Flurkey, Austen Te, David E. Harrison · 2016

Numerous studies suggest that rapamycin treatment promotes insulin resistance, implying that rapamycin could have negative effects on patients with, or at risk for, type 2 diabetes (T2D). New evidence, however, indicates that rapamycin tre…

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