Peter Czermak
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View article: Fouling Control of Ion-Selective Electrodes (ISEs) in Aquatic and Aquacultural Environments: A Comprehensive Review
Fouling Control of Ion-Selective Electrodes (ISEs) in Aquatic and Aquacultural Environments: A Comprehensive Review Open
Real-time monitoring is essential for maintaining water quality and optimizing aquaculture productivity. Ion-selective electrodes (ISEs) are widely used to measure key parameters such as pH, nitrate, and dissolved oxygen in aquatic environ…
View article: Efficient Separation of a Novel Microbial Chassis, Vibrio natriegens, from High-Salt Culture Broth Using Ceramic Ultrafiltration Membranes
Efficient Separation of a Novel Microbial Chassis, Vibrio natriegens, from High-Salt Culture Broth Using Ceramic Ultrafiltration Membranes Open
Vibrio natriegens is widely used as a production host for biotechnological processes due to its superior maximum glucose consumption rate, high growth rate, and abundant ribosomes. Most bioprocesses also need a scalable biomass separation …
View article: The Occurrence of Micropollutants in the Aquatic Environment and Technologies for Their Removal
The Occurrence of Micropollutants in the Aquatic Environment and Technologies for Their Removal Open
The presence of micropollutants in aquatic environments is an increasing global concern due to their persistence and potential harmful effects on aquatic organisms. Among the most concerning of these micropollutants are microplastics, phar…
View article: Efficient Separation of Novel Microbial Chassis Vibrio natriegens from the High-Salt Culture Broth Using Ceramic Ultrafiltration Membranes
Efficient Separation of Novel Microbial Chassis Vibrio natriegens from the High-Salt Culture Broth Using Ceramic Ultrafiltration Membranes Open
Vibrio natriegens is widely used as a production host for biotechnological processes due to its superior maximum glucose consumption rate, high growth rate, and abundant ribosomes. Most bioprocesses also need a scalable biomass separation …
View article: The Occurrence of Micropollutants in the Aquatic Environment and their Removal Technologies
The Occurrence of Micropollutants in the Aquatic Environment and their Removal Technologies Open
Pharmaceutical compounds in aquatic environments are recognized as emerging contaminants due to their potential risks to living organisms. The growing accessibility, widespread use, and disposal of pharmaceuticals have led to a significant…
View article: Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry
Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry Open
Background: The efficiency of recombinant protein production in Yarrowia lipolytica is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically…
View article: Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection
Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection Open
Oncolytic measles virus (MeV) is a promising anti-cancer treatment. However, the production of high titers of infectious MeV (typically 107–109 TCID50 per dose) is challenging because the virus is unstable under typical production conditio…
View article: Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection
Production of Oncolytic Measles Virus in Vero Cells: Impact of Culture Medium and Multiplicity of Infection Open
Oncolytic measles virus (MeV) is a promising anti-cancer treatment. However, the production of high titers of infectious MeV (typically 107–109 TCID50 per dose) is challenging because the virus is unstable under typical production co…
View article: Large-Scale Expansion of Human Liver Stem Cells Using Two Different Bioreactor Systems
Large-Scale Expansion of Human Liver Stem Cells Using Two Different Bioreactor Systems Open
The assessment of human liver stem cells (HLSCs) as cell therapeutics requires scalable, controlled expansion processes. We first focused on defining appropriate process parameters for HLSC expansion such as seeding density, use of antibio…
View article: Process development for the production of mesenchymal stromal cell-derived extracellular vesicles in conventional 2D systems
Process development for the production of mesenchymal stromal cell-derived extracellular vesicles in conventional 2D systems Open
Our findings suggest that parameters, which must be defined prior to each individual cultivation and do not require complex and expensive equipment, can significantly increase MSC-derived particle production including EVs. Integrating thes…
View article: Production of Fructo‐Oligosaccharides in Membrane Bioreactor Using Rotating Ceramic Membrane Disc
Production of Fructo‐Oligosaccharides in Membrane Bioreactor Using Rotating Ceramic Membrane Disc Open
Fructo‐oligosaccharides (FOS) are often produced via enzymatic conversion in industry. Enzyme membrane bioreactor (EMBR) can enhance the catalytic efficiency by in‐situ product removal. Its major bottleneck is the fouling caused by the dep…
View article: A Bioreactor-Based Yellow Fever Virus-like Particle Production Process with Integrated Process Analytical Technology Based on Transient Transfection
A Bioreactor-Based Yellow Fever Virus-like Particle Production Process with Integrated Process Analytical Technology Based on Transient Transfection Open
Yellow Fever (YF) is a severe disease that, while preventable through vaccination, lacks rapid intervention options for those already infected. There is an urgent need for passive immunization techniques using YF-virus-like particles (YF-V…
View article: Depolymerization of hemicelluloses utilizing hydrothermal and acid catalyzed processes proceed by ultrafiltration as fractionation media
Depolymerization of hemicelluloses utilizing hydrothermal and acid catalyzed processes proceed by ultrafiltration as fractionation media Open
Replacement of fossil-based polymers is a current challenge, which needs a broad feedstock of bio-based substitutes produced with a reasonable CO2-footprint. As indigestible biopolymers ß-1,4-hemicelluloses were extracted with an extractio…
View article: Identification and characterisation of the tegument-expressed aldehyde dehydrogenase SmALDH_312 of Schistosoma mansoni, a target of disulfiram
Identification and characterisation of the tegument-expressed aldehyde dehydrogenase SmALDH_312 of Schistosoma mansoni, a target of disulfiram Open
Schistosomiasis is an infectious disease caused by blood flukes of the genus Schistosoma and affects approximately 200 million people worldwide. Since Praziquantel (PZQ) is the only drug for schistosomiasis, alternatives are needed. By a b…
View article: Dynamic expansion of mesenchymal stem/stromal cells in a stirred tank bioreactor promotes the release of potent extracellular vesicles
Dynamic expansion of mesenchymal stem/stromal cells in a stirred tank bioreactor promotes the release of potent extracellular vesicles Open
Mesenchymal stem/stromal cell-derived extracellular vesicles (MSC-EVs) are considered a promising therapeutic tool in cell therapy due to their immunomodulatory, regenerative and angiogenic capabilities. However, there is a lack of proces…
View article: A New Control Strategy for High-Pressure Homogenization to Improve the Safety of Injectable Lipid Emulsions
A New Control Strategy for High-Pressure Homogenization to Improve the Safety of Injectable Lipid Emulsions Open
Intravenous lipid emulsions are biocompatible formulations used as clinical nutrition products and lipid-based delivery systems for sparingly soluble drugs. However, the particle-size distribution is associated with risks of embolism. Acco…
View article: Process Analytical Technology for the Production of Parenteral Lipid Emulsions According to Good Manufacturing Practices
Process Analytical Technology for the Production of Parenteral Lipid Emulsions According to Good Manufacturing Practices Open
The good manufacturing practices (GMP) and process analytical technology (PAT) initiatives of the US Food and Drug Administration, in conjunction with International Council for Harmonisation (ICH) quality guidelines Q8, Q9, and Q10, ensure…
View article: Hot-Water Hemicellulose Extraction from Fruit Processing Residues
Hot-Water Hemicellulose Extraction from Fruit Processing Residues Open
Hemicelluloses are an abundant biopolymer resource with interesting properties for applications in coatings and composite materials. The objective of this investigation was to identify variables of industrially relevant extraction processe…
View article: Screening for Biofilm-Stimulating Factors in the Freshwater Planctomycete Planctopirus limnophila to Improve Sessile Growth in a Chemically Defined Medium
Screening for Biofilm-Stimulating Factors in the Freshwater Planctomycete Planctopirus limnophila to Improve Sessile Growth in a Chemically Defined Medium Open
Planctomycetes such as Planctopirus limnophila offer a promising source of bioactive molecules, particularly when they switch from planktonic to sessile growth, but little is known about the corresponding biosynthetic gene clusters and how…
View article: GbpA as a secretion and affinity purification tag for an antimicrobial peptide produced in Vibrio natriegens
GbpA as a secretion and affinity purification tag for an antimicrobial peptide produced in Vibrio natriegens Open
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View article: The effect of different insect cell culture media on the efficiency of protein production by Spodoptera frugiperda cells
The effect of different insect cell culture media on the efficiency of protein production by Spodoptera frugiperda cells Open
Background: Spodoptera frugiperda (Sf-9) cells are often used to produce recombinant proteins by transient expression. The culture medium contributes to the efficiency of protein production, but the effect of modern insect cell culture med…
View article: A Combined Ultrafiltration/Diafiltration Process for the Purification of Oncolytic Measles Virus
A Combined Ultrafiltration/Diafiltration Process for the Purification of Oncolytic Measles Virus Open
Measles virus (MV) is an important representative of a new class of cancer therapeutics known as oncolytic viruses. However, process intensification for the downstream purification of this fragile product is challenging. We previously foun…
View article: Isolation and Purification of a Hydrophobic Non-Ribosomal Peptide from an Escherichia coli Fermentation Broth
Isolation and Purification of a Hydrophobic Non-Ribosomal Peptide from an Escherichia coli Fermentation Broth Open
Non-ribosomal peptide synthases (NRPSs) generate versatile bioactive peptides by incorporating non-proteinogenic amino acids and catalyzing diverse modifications. Here, we developed an efficient downstream process for the capture, intermed…
View article: Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
Development of a chemically defined medium for Planctopirus limnophila to increase biomass production Open
Background Planctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media ach…
View article: Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system
Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system Open
Background: The heterologous expression of parasitic proteins is challenging because the sequence composition often differs significantly from host preferences. However, the production of such proteins is important because they are potenti…
View article: Purification of oncolytic measles virus by cation-exchange chromatography using resin-based stationary phases
Purification of oncolytic measles virus by cation-exchange chromatography using resin-based stationary phases Open
Cancer therapy with oncolytic measles virus (MV) requires high titers of pure, infectious virus particles (~108 times more particles per dose than the measles vaccine). A gentle but efficient downstream purification process is therefore es…