Peter Dröge
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View article: Targeting FANCM by antisense oligonucleotides in ALT-positive cancers
Targeting FANCM by antisense oligonucleotides in ALT-positive cancers Open
Effective therapies for cancers relying on the alternative lengthening of telomeres (ALT) mechanisms are still needed. Here, using CRISPR-Cas9 strategies, we validate FANCM (Fanconi anemia complementation group M) as a crucial target for A…
View article: Genetic and pharmacological modulation of lamin A farnesylation determines its function and turnover
Genetic and pharmacological modulation of lamin A farnesylation determines its function and turnover Open
Hutchinson–Gilford Progeria syndrome (HGPS) is a severe premature ageing disorder caused by a 50 amino acid truncated (Δ50AA) and permanently farnesylated lamin A (LA) mutant called progerin. On a cellular level, progerin expression leads …
View article: Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences
Expanding the DNA editing toolbox: Novel lambda integrase variants targeting microalgal and human genome sequences Open
Recombinase enzymes are extremely efficient at integrating very large DNA fragments into target genomes. However, intrinsic sequence specificities curtail their use to DNA sequences with sufficient homology to endogenous target motifs. Ext…
View article: Expanding the DNA editing toolbox: novel lambda integrase variants targeting microalgal and human genome sequences
Expanding the DNA editing toolbox: novel lambda integrase variants targeting microalgal and human genome sequences Open
Recombinase enzymes are extremely efficient at integrating very large DNA fragments into target genomes. However, intrinsic sequence specificities curtail their use to DNA sequences with sufficient homology to endogenous target motifs. Ext…
View article: A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells
A versatile genomic transgenesis platform with enhanced λ integrase for human Expi293F cells Open
Reliable cell-based platforms to test and/or produce biologics in a sustainable manner are important for the biotech industry. Utilizing enhanced λ integrase, a sequence-specific DNA recombinase, we developed a novel transgenesis platform …
View article: Signalling inhibition by ponatinib disrupts productive alternative lengthening of telomeres (ALT)
Signalling inhibition by ponatinib disrupts productive alternative lengthening of telomeres (ALT) Open
Alternative lengthening of telomeres (ALT) supports telomere maintenance in 10–15% of cancers, thus representing a compelling target for therapy. By performing anti-cancer compound library screen on isogenic cell lines and using extrachrom…
View article: Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase
Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase Open
Seamless DNA vectors derived from bacterial plasmids are devoid of bacterial genetic elements and represent attractive alternatives for biomedical applications including DNA vaccines. Larger scale production of seamless vectors employs eng…
View article: Interferon β, an enhancer of the innate immune response against SARS-CoV-2 infection
Interferon β, an enhancer of the innate immune response against SARS-CoV-2 infection Open
View article: A non-viral genome editing platform for site-specific insertion of large transgenes
A non-viral genome editing platform for site-specific insertion of large transgenes Open
Background The precise, functional and safe insertion of large DNA payloads into host genomes offers versatility in downstream genetic engineering-associated applications, spanning cell and gene therapies, therapeutic protein production, h…
View article: CCDC 1967684: Experimental Crystal Structure Determination
CCDC 1967684: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1967685: Experimental Crystal Structure Determination
CCDC 1967685: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1967683: Experimental Crystal Structure Determination
CCDC 1967683: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility
Parallel bimodal single-cell sequencing of transcriptome and chromatin accessibility Open
Joint profiling of transcriptome and chromatin accessibility within single cells allows for the deconstruction of the complex relationship between transcriptional states and upstream regulatory programs determining different cell fates. He…
View article: Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication
Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication Open
Key DNA transactions, such as genome replication and transcription, rely on the speedy translocation of specialized protein complexes along a double-stranded, right-handed helical template. Physical tethering of these molecular machines du…
View article: IHF stabilizes pathogenicity island I of uropathogenic Escherichia coli strain 536 by attenuating integrase I promoter activity
IHF stabilizes pathogenicity island I of uropathogenic Escherichia coli strain 536 by attenuating integrase I promoter activity Open
View article: Discovery of a Novel Mycobacterial F‐ATP Synthase Inhibitor and its Potency in Combination with Diarylquinolines
Discovery of a Novel Mycobacterial F‐ATP Synthase Inhibitor and its Potency in Combination with Diarylquinolines Open
The F 1 F O ‐ATP synthase is required for growth and viability of Mycobacterium tuberculosis and is a validated clinical target. A mycobacterium‐specific loop of the enzyme's rotary γ subunit plays a role in the coupling of ATP synthesis w…
View article: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology Open
Rapidly dividing cells maintain chromatin supercoiling homeostasis via two specialized classes of enzymes, DNA topoisomerase type 1 and 2 (TOP1/2). Several important anticancer drugs perturb this homeostasis by targeting TOP1/2, thereby ge…
View article: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity Open
The transient build-up of DNA supercoiling during the translocation of replication forks threatens genome stability and is controlled by DNA topoisomerases (TOPs). This crucial process has been exploited with TOP poisons for cancer chemoth…
View article: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity Open
The transient build-up of DNA supercoiling during the translocation of replication forks threatens genome stability and is controlled by DNA topoisomerases (TOPs). This crucial process has been exploited with TOP poisons for cancer chemoth…
View article: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis Open
Telomerase RNA (TR) provides the template for DNA repeat synthesis at telomeres and is essential for genome stability in continuously dividing cells. We mapped the RNA interactome of human TR (hTR) and identified a set of non-coding and co…
View article: Author response: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
Author response: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis Open
View article: A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression
A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression Open
Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge.…
View article: Publisher Correction: Utf1 contributes to intergenerational epigenetic inheritance of pluripotency
Publisher Correction: Utf1 contributes to intergenerational epigenetic inheritance of pluripotency Open
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
View article: Utf1 contributes to intergenerational epigenetic inheritance of pluripotency
Utf1 contributes to intergenerational epigenetic inheritance of pluripotency Open
View article: Oncofetal HMGA2 effectively curbs unconstrained (+) and (−) DNA supercoiling
Oncofetal HMGA2 effectively curbs unconstrained (+) and (−) DNA supercoiling Open
View article: Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I
Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I Open
HMGA2 is an important chromatin factor that interacts with DNA via three AT-hook domains, thereby regulating chromatin architecture and transcription during embryonic and fetal development. The protein is absent from differentiated somatic…
View article: Conservative site-specific and single-copy transgenesis in human<i>LINE-1</i>elements
Conservative site-specific and single-copy transgenesis in human<i>LINE-1</i>elements Open
Genome engineering of human cells plays an important role in biotechnology and molecular medicine. In particular, insertions of functional multi-transgene cassettes into suitable endogenous sequences will lead to novel applications. Althou…
View article: Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF
Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF Open
View article: Stereochemical control of nucleosome targeting by platinum-intercalator antitumor agents
Stereochemical control of nucleosome targeting by platinum-intercalator antitumor agents Open
Platinum-based anticancer drugs act therapeutically by forming DNA adducts, but suffer from severe toxicity and resistance problems, which have not been overcome in spite of decades of research. And yet defined chromatin targets have gener…
View article: Directed evolution of λ integrase activity and specificity by genetic derepression
Directed evolution of λ integrase activity and specificity by genetic derepression Open
Advances in genome engineering are attendant on the development of novel enzyme variants with programed substrate specificities and improved activity. We have devised a novel selection method, wherein the activity of a recombinase deletes …