Peter J. Halling
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View article: Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements
Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements Open
The measurement of values of apparent equilibrium constants K ′ for enzyme-catalyzed reactions involve a substantial number of critical details, neglect of which could lead to systematic errors. Here, interferences, impurities in the subst…
View article: Kinetics of enzyme-catalysed desymmetrisation of prochiral substrates: product enantiomeric excess is not always constant
Kinetics of enzyme-catalysed desymmetrisation of prochiral substrates: product enantiomeric excess is not always constant Open
The kinetics of enzymatic desymmetrisation were analysed for the most common kinetic mechanisms: ternary complex ordered (prochiral ketone reduction); ping-pong second (ketone amination, diol esterification, desymmetrisation in the second …
View article: Thermodynamic Favorability of End Products of Anaerobic Glucose Metabolism
Thermodynamic Favorability of End Products of Anaerobic Glucose Metabolism Open
The eQuilibrator component contribution method allows calculation of the overall Gibbs energy changes for conversion of glucose to a wide range of final products in the absence of other oxidants. Values are presented for all possible combi…
View article: Highly Active Protein Surfaces Enabled by Plant-Based Polyphenol Coatings
Highly Active Protein Surfaces Enabled by Plant-Based Polyphenol Coatings Open
Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing and industrial biocatalysis to biomedical interf…
View article: STRENDA DB: enabling the validation and sharing of enzyme kinetics data
STRENDA DB: enabling the validation and sharing of enzyme kinetics data Open
This FAIRsharing record describes: STRENDA DB is a storage and search platform supported by the Beilstein-Institut that incorporates the STRENDA Guidelines in a user-friendly, web-based system. If you are an author who is preparing a manus…
View article: Robust Sampling Time Design for Biochemical Systems
Robust Sampling Time Design for Biochemical Systems Open
Optimal sampling time design by considering parameter uncertainties has rarely been considered in published research. In this work, the robust experimental design (RED) for sampling time selection is investigated. The aim is to exploit the…
View article: High-Resolution Structural Characterization of a Heterogeneous Biocatalyst Using Solid-State NMR
High-Resolution Structural Characterization of a Heterogeneous Biocatalyst Using Solid-State NMR Open
Solid-state magic-angle spinning (MAS) NMR spectroscopy was employed to investigate structural detail in the enzyme human carbonic anhydrase II (hCA II) in uniformly N-15 and selectively (N-15 leucine) enriched states, covalently immobiliz…
View article: Secondary structure content (%) as determined by K2D2 software from the Far-UV CD spectra as given in Figure 6A.
Secondary structure content (%) as determined by K2D2 software from the Far-UV CD spectra as given in Figure 6A. Open
Secondary structure content (%) as determined by K2D2 software from the Far-UV CD spectra as given in Figure 6A.
View article: Enzymatic Synthesis of Glucose-Based Fatty Acid Esters in Bisolvent Systems Containing Ionic Liquids or Deep Eutectic Solvents
Enzymatic Synthesis of Glucose-Based Fatty Acid Esters in Bisolvent Systems Containing Ionic Liquids or Deep Eutectic Solvents Open
Sugar fatty acid esters (SFAEs) are biocompatible nonionic surfactants with broad applications in food, cosmetic, and pharmaceutical industries. They can be synthesized enzymatically with many advantages over their chemical synthesis. In t…
View article: Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity
Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity Open
As nonionic surfactants derived from naturally renewable resources, sugar fatty acid esters (SFAEs) have been widely utilized in food, cosmetic, and pharmaceutical industries. In this study, six enzymes were screened as catalyst for synthe…
View article: A Two-loop Optimization Strategy for Multi-objective Optimal Experimental Design
A Two-loop Optimization Strategy for Multi-objective Optimal Experimental Design Open
A new strategy of optimal experimental design (OED) is proposed for a kinetically controlled synthesis system by considering both the observation design and the input design. The observation design that combines sampling scheduling and mea…