Peter McCourt
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View article: PACA nanoparticles target and deliver sildenafil to rejuvenate aged mouse liver sinusoidal endothelial cells
PACA nanoparticles target and deliver sildenafil to rejuvenate aged mouse liver sinusoidal endothelial cells Open
Ageing is established as the most significant risk factor for disease. About 75% of people over 75 years have diabetes or pre-diabetes and/or hyperlipidaemia which are established risk factors for cardiovascular outcomes, and risk factors …
View article: Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation
Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation Open
Liver sinusoidal endothelial cells (LSECs) play a crucial role in hepatic homeostasis, clearance, and microcirculatory regulation. Their fenestrations—patent transcellular pores—are essential for proper liver function, yet disappear in pat…
View article: Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips
Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips Open
Super‐resolution optical microscopy (SRM) permits the visualization of subcellular structures of biological samples beyond the diffraction limit of light. To evaluate and utilize the specific strengths of each SRM technique a combined appr…
View article: Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function
Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function Open
Atherogenesis is associated with elevated plasma levels of oxidized low-density lipoproteins (oxLDL). In vivo, oxLDL causes liver endothelial swelling, and disrupts liver sinusoidal endothelial cell (LSECs) fenestrations. We mapped the nan…
View article: Liver sinusoidal cells eliminate blood-borne phage K1F
Liver sinusoidal cells eliminate blood-borne phage K1F Open
Phage treatment has regained attention due to an increase in multiresistant bacteria. For phage therapy to be successful, phages must reach their target bacteria in sufficiently high numbers. Blood-borne phages are believed to be captured …
View article: The Computed Sinusoid
The Computed Sinusoid Open
Hepatic sinusoids are lined with thin endothelial cells with transcellular pores, termed fenestrations. These fenestrations are open channels that connect the sinusoidal lumen to the underlying Space of Disse (SoD) and the hepatocytes of t…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: The Computed Sinusoid
The Computed Sinusoid Open
The hepatic sinusoids are lined by thin endothelium containing transcellular pores dubbed fenestra. These fenestrations are completely open channels connecting the sinusoidal lumen to the underlying Space of Disse (SoD) and the hepatocytes…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope
Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope Open
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in the back focal plane of the objective lens affects the pattern periodicity, and, thus, the resulting spatial resolution. Here, w…
View article: Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure
Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure Open
Xanthines such as caffeine and theobromine are among the most consumed psychoactive stimulants in the world, either as natural components of coffee, tea and chocolate, or as added ingredients. The present study assessed if xanthines affect…
View article: Highly oxidized albumin is mainly cleared by mouse liver sinusoidal endothelial cells via the receptors stabilin-1 and -2
Highly oxidized albumin is mainly cleared by mouse liver sinusoidal endothelial cells via the receptors stabilin-1 and -2 Open
Background Oxidized albumin (oxHSA) is elevated in several pathological conditions, especially those involving the liver, such as decompensated cirrhosis, acute on chronic liver failure and liver mediated renal failure. Patient derived oxi…
View article: High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components
High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components Open
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are …
View article: High-speed TIRF and 2D super-resolution structured illumination microscopy with large field of view based on fiber optic components
High-speed TIRF and 2D super-resolution structured illumination microscopy with large field of view based on fiber optic components Open
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are …
View article: Oral nanotherapeutic formulation of insulin with reduced episodes of hypoglycaemia
Oral nanotherapeutic formulation of insulin with reduced episodes of hypoglycaemia Open
Injectable insulin is an extensively used medication with potential life-threatening hypoglycaemic events. Here we evaluated silver sulfide quantum dots (QDs) with a chitosan/glucose (CS/GS) encapsulating polymer to produce an oral insulin…
View article: Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure
Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure Open
Xanthines such as caffeine and theobromine are among the most consumed psychoactive stimulants in the world, either as natural components of coffee, tea and chocolate, or as food additives. The present study assessed if xanthines affect li…
View article: Dual-color single molecule localization microscopy on transparent polymer waveguide chips
Dual-color single molecule localization microscopy on transparent polymer waveguide chips Open
Photonic waveguide chips offer near-field excitation of biological samples, which enables cost-effective, large field-of-view super-resolution microscopy without the need for high numerical aperture (NA) objective lenses. Single molecule l…
View article: Tuning of Liver Sieve: The Interplay between Actin and Myosin Regulatory Light Chain Regulates Fenestration Size and Number in Murine Liver Sinusoidal Endothelial Cells
Tuning of Liver Sieve: The Interplay between Actin and Myosin Regulatory Light Chain Regulates Fenestration Size and Number in Murine Liver Sinusoidal Endothelial Cells Open
Liver sinusoidal endothelial cells (LSECs) facilitate the efficient transport of macromolecules and solutes between the blood and hepatocytes. The efficiency of this transport is realized via transcellular nanopores, called fenestrations. …
View article: From fixed-dried to wet-fixed to live – comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations
From fixed-dried to wet-fixed to live – comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations Open
Fenestrations in liver sinusoidal endothelial cells (LSEC) are transcellular nanopores of 50–350 nm diameter that facilitate bidirectional transport of solutes and macromolecules between the bloodstream and the parenchyma of the liver. Liv…
View article: Opportunities and Challenges for Nanotherapeutics for the Aging Population
Opportunities and Challenges for Nanotherapeutics for the Aging Population Open
Nanotherapeutics utilize the properties of nanomaterials to alter the pharmacology of the drugs and therapies being transported, leading to changes in their biological disposition (absorption, distribution, cellular uptake, metabolism and …
View article: Autofluorescence in freshly isolated adult human liver sinusoidal cells
Autofluorescence in freshly isolated adult human liver sinusoidal cells Open
Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysoso…
View article: Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells
Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells Open
Significance We propose the integration of chip-based optical nanoscopy with high spatially sensitive quantitative phase microscopy to obtain three-dimensional (3D) morphology of liver sinusoidal endothelial cells (LSEC). LSEC contain larg…
View article: The Scavenger Function of Liver Sinusoidal Endothelial Cells in Health and Disease
The Scavenger Function of Liver Sinusoidal Endothelial Cells in Health and Disease Open
The aim of this review is to give an outline of the blood clearance function of the liver sinusoidal endothelial cells (LSECs) in health and disease. Lining the hundreds of millions of hepatic sinusoids in the human liver the LSECs are per…
View article: The wHole Story About Fenestrations in LSEC
The wHole Story About Fenestrations in LSEC Open
The porosity of liver sinusoidal endothelial cells (LSEC) ensures bidirectional passive transport of lipoproteins, drugs and solutes between the liver capillaries and the liver parenchyma. This porosity is realized via fenestrations – tran…