Peter Saggau
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View article: Acousto-optic deflectors in experimental neuroscience: overview of theory and applications
Acousto-optic deflectors in experimental neuroscience: overview of theory and applications Open
Cutting-edge methodologies and techniques are required to understand complex neuronal dynamics and pathological mechanisms. Among them, optical tools stand out due to their combination of non-invasiveness, speed, and precision. Examples in…
Acousto-Optic Devices in Advanced Microscopy Open
This review presents several advanced microscope designs employing acousto-optic deflectors (AODs).Here, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens.This …
Scan-less microscopy based on acousto-optic encoded illumination Open
Several optical microscopy methods are now available for characterizing scientific and industrial processes at sub-micron resolution. However, they are often ill-suited for imaging rapid events. Limited by the trade-off between camera fram…
Issue Information Open
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Multiplane Encoded Light-Sheet Microscopy for Enhanced 3D Imaging Open
Light-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodam…
Acousto-optic systems for advanced microscopy Open
Acoustic waves in an optical medium cause rapid periodic changes in the refraction index, leading to diffraction effects. Such acoustically controlled diffraction can be used to modulate, deflect, and focus light at microsecond timescales,…
View article: WITHDRAWN: Multiplane Mesoscope reveals distinct cortical interactions following expectation violations
WITHDRAWN: Multiplane Mesoscope reveals distinct cortical interactions following expectation violations Open
Withdrawal Statement This manuscript has been withdrawn because it was posted without the consent of all authors. Therefore, this work should not be cited as reference for the project. If you have any questions, please contact the correspo…
An inertia-free beam scanning device for single-wavelength 2PE-STED nanoscopy Open
Two-photon excitation stimulated emission depletion nanoscopy (2PE-STED) is a fluorescence imaging technique ideal for significantly improving the spatial resolution when observing scattering tissue in fixed, in vitro , and in vivo specime…
Aberration-free multi-plane imaging of neural activity from the mammalian brain using a fast-switching liquid crystal spatial light modulator Open
We report a novel two-photon fluorescence microscope based on a fast-switching liquid crystal spatial light modulator and a pair of galvo-resonant scanners for large-scale recording of neural activity from the mammalian brain. The spatial …
Two-photon frequency division multiplexing for functional in vivo imaging: a feasibility study Open
Recently, we presented a new approach to create high-speed amplitude modulation of femtosecond laser pulses and tag multiple excitation beams with specific modulation frequencies. In this work, we discuss the utility of this method to reco…
Multi-plane Imaging of Neural Activity From the Mammalian Brain Using a Fast-switching Liquid Crystal Spatial Light Modulator Open
We report a novel two-photon fluorescence microscope based on a fast-switching liquid crystal spatial light modulator and a pair of galvo-resonant scanners for large-scale recording of neural activity from the mammalian brain. The utilized…
Two-photon frequency division multiplexing for functional <i>in vivo</i> imaging: a feasibility study Open
Recently, we presented a new approach to create high-speed amplitude modulation of femtosecond laser pulses and tag multiple excitation beams with specific modulation frequencies. In this work, we discuss the utility of this method to reco…
SmartScope2: Simultaneous Imaging and Reconstruction of Neuronal Morphology Open
Quantitative analysis of neuronal morphology is critical in cell type classification and for deciphering how structure gives rise to function in the brain. Most current approaches to imaging and tracing neuronal 3D morphology are data inte…
Fast Inertia-Free Volumetric Light-Sheet Microscope Open
Fast noninvasive three-dimensional (3D) imaging is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characteriza…
Fast Inertia-Free Volumetric Light-Sheet Microscope Open
Fast noninvasive three-dimensional (3D) imag-ing is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characteriz…
Amplitude modulation of femtosecond laser pulses in the megahertz range for frequency-multiplexed two-photon imaging Open
We present a frequency-multiplexed multi-site two-photon imaging method utilizing amplitude modulation of femtosecond laser pulses in the MHz range to tag each excitation beam and the corresponding fluorescence signals with specific freque…
SmartScope2: Simultaneous Imaging and Reconstruction of Neuronal Morphology Open
Quantitative analysis of neuronal morphology is critical in cell type classification and for deciphering how structure gives rise to function in the brain. Most current approaches to imaging and tracing neuronal 3D morphology are data inte…