Petr Chlanda
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View article: Visualizing influenza A virus assembly by in situ cryo-electron tomography
Visualizing influenza A virus assembly by in situ cryo-electron tomography Open
Influenza A virus (IAV) forms pleomorphic particles that package eight ribonucleoprotein complexes (vRNPs), each carrying a distinct RNA genome segment. vRNPs assemble in the nucleus and undergo selective sorting during Rab11a-mediated tra…
View article: A novel accessory gene product of tick-borne Dhori-Orthomyxovirus, encoded by overlooked spliced transcripts of RNA segment 6
A novel accessory gene product of tick-borne Dhori-Orthomyxovirus, encoded by overlooked spliced transcripts of RNA segment 6 Open
Dhori virus (DHOV) ( Orthomyxoviridae, genus: Thogotovirus) is a tick-borne virus with a segmented, negative-sense RNA genome. Thogotoviruses can be divided into two clades, Thogoto-like and Dhori-like viruses, which differ in their coding…
View article: Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike
Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike Open
View article: Performance and feasibility of self-microsampling of capillary blood and saliva for serological testing of SARS-CoV-2
Performance and feasibility of self-microsampling of capillary blood and saliva for serological testing of SARS-CoV-2 Open
Background Serology is a valuable tool to estimate infections, case-fatality rates, and immunity. However, venipuncture and clinical infrastructure hinder scalability. This study evaluated the performance, feasibility and user experience o…
View article: Morphology-dependent entry kinetics and spread of influenza A virus
Morphology-dependent entry kinetics and spread of influenza A virus Open
Influenza A viruses (IAV) display a broad variety of morphologies ranging from spherical to long filamentous virus particles. These diverse phenotypes are believed to allow the virus to overcome various immunological and pulmonary barriers…
View article: On-lamella dual-axis cryo-electron tomography and modelling of lamella stability
On-lamella dual-axis cryo-electron tomography and modelling of lamella stability Open
Cryogenic electron tomography (cryo-ET) of cryogenic focused ion beam (cryo-FIB) milled lamellae enables the study of 3D cellular architecture in near-native conditions at nanometer-scale resolution. Cryo-FIB milling is performed iterative…
View article: K5 polysaccharides inhibit SARS-CoV-2 infection by preventing spike-proteolytic priming
K5 polysaccharides inhibit SARS-CoV-2 infection by preventing spike-proteolytic priming Open
SARS-CoV-2 spike glycoprotein is a promising drug target due to its crucial role in viral infection. Heparin, a long linear polysaccharide that inhibits SARS-CoV-2 infection by acting on spike, has limited antiviral applications due to its…
View article: To be or not to be phosphorylated: understanding the role of Ebola virus nucleoprotein in the dynamic interplay with the transcriptional activator VP30 and the host phosphatase PP2A-B56
To be or not to be phosphorylated: understanding the role of Ebola virus nucleoprotein in the dynamic interplay with the transcriptional activator VP30 and the host phosphatase PP2A-B56 Open
Ebola virus (EBOV) transcription is essentially regulated via dynamic dephosphorylation of its viral transcription activator VP30 by the host phosphatase PP2A. The nucleoprotein NP has emerged as a third key player in the regulation of thi…
View article: T-cell specific in vivo gene delivery with DART-AAVs targeted to CD8
T-cell specific in vivo gene delivery with DART-AAVs targeted to CD8 Open
View article: Morphology-dependent entry kinetics and spread of influenza A virus
Morphology-dependent entry kinetics and spread of influenza A virus Open
Influenza A viruses (IAV) display a broad variety of morphologies ranging from spherical to long filamentous virus particles. These diverse phenotypes are believed to allow the virus to overcome various immunological and pulmonary barriers…
View article: Influenza A virus hemagglutinin remodels membranes into a vRNP clustering platform
Influenza A virus hemagglutinin remodels membranes into a vRNP clustering platform Open
Pandemics caused by influenza A virus are associated with co-infections of zoonotic and human-adapted viruses. To form an infectious entity, the virus must incorporate eight different ribonucleoproteins (vRNPs) into virions budding at the …
View article: Glucosylceramide in bunyavirus particles is essential for virus binding to host cells
Glucosylceramide in bunyavirus particles is essential for virus binding to host cells Open
View article: Author Response: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Author Response: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Full text Figures and data Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Abstract Fibroblast growth fact…
View article: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast growth factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by phosphatidylinositol-4,5-bisphosphate (PI(4,5)P 2 )-depend…
View article: SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle
SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle Open
Coronavirus replication is associated with the remodeling of cellular membranes, resulting in the formation of double-membrane vesicles (DMVs). A DMV-spanning pore was identified as a putative portal for viral RNA. However, the exact compo…
View article: A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses
A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses Open
In vivo, HIV-1 replicates within tissue, but how three-dimensional (3D) tissue-like environments influence viral spread and pathogenesis remains largely unknown. We previously identified an Environmental Restriction to cell-free Virus Infe…
View article: AAV vectors displaying bispecific DARPins enable dual-control targeted gene delivery
AAV vectors displaying bispecific DARPins enable dual-control targeted gene delivery Open
View article: Author Response: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Author Response: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FGF2 oligo…
View article: Reviewer #3 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Reviewer #3 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FGF2 oligo…
View article: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Summary Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FG…
View article: Reviewer #1 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Reviewer #1 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FGF2 oligo…
View article: Reviewer #2 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Reviewer #2 (Public Review): Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FGF2 oligo…
View article: Nucleocapsid condensation drives Ebola viral factory maturation and dispersion
Nucleocapsid condensation drives Ebola viral factory maturation and dispersion Open
Summary Replication and genome encapsidation of many negative-sense RNA viruses take place in virus-induced membrane-less organelles termed viral factories (VFs). While liquid properties of VFs are believed to control the transition from g…
View article: SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 Open
Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-…
View article: Cryo-electron tomography of viral infection — from applications to biosafety
Cryo-electron tomography of viral infection — from applications to biosafety Open
Cellular cryo-electron tomography (cryo-ET) offers 3D snapshots at molecular resolution capturing pivotal steps during viral infection. However, tomogram quality depends on the vitrification level of the sample and its thickness. In additi…
View article: Ebola virus inclusion bodies are liquid organelles whose formation is facilitated by nucleoprotein oligomerization
Ebola virus inclusion bodies are liquid organelles whose formation is facilitated by nucleoprotein oligomerization Open
Viral RNA synthesis of several non-segmented, negative-sense RNA viruses (NNSVs) takes place in inclusion bodies (IBs) that show properties of liquid organelles, which are formed by liquid–liquid phase separation of scaffold proteins. It i…
View article: Joint Public Review: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Joint Public Review: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FGF2 oligo…
View article: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Summary Fibroblast Growth Factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by PI(4,5)P2-dependent formation of highly dynamic FG…
View article: Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space
Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space Open
Fibroblast growth factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by phosphatidylinositol-4,5-bisphosphate (PI(4,5)P 2 )-depend…
View article: The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion
The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion Open
Ebola viruses (EBOVs) assemble into filamentous virions, whose shape and stability are determined by the matrix viral protein 40 (VP40). Virus entry into host cells occurs via membrane fusion in late endosomes; however, the mechanism of ho…