Petr Fajkus
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View article: Telomerase RNA evolution: a journey from plant telomeres to broader eukaryotic diversity
Telomerase RNA evolution: a journey from plant telomeres to broader eukaryotic diversity Open
Telomeres, essential for maintaining genomic stability, are typically preserved through the action of telomerase, a ribonucleoprotein complex that synthesizes telomeric DNA. One of its two core components, telomerase RNA (TR), serves as th…
View article: Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex
Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex Open
View article: TeloBase: a community-curated database of telomere sequences across the tree of life
TeloBase: a community-curated database of telomere sequences across the tree of life Open
Discoveries over the recent decade have demonstrated the unexpected diversity of telomere DNA motifs in nature. However, currently available resources, ‘Telomerase database’ and ‘Plant rDNA database’, contain just fragments of all relevant…
View article: Telomerase <scp>RNA</scp> gene paralogs in plants – the usual pathway to unusual telomeres
Telomerase <span>RNA</span> gene paralogs in plants – the usual pathway to unusual telomeres Open
Summary Telomerase, telomeric DNA and associated proteins together represent a complex, finely tuned and functionally conserved mechanism that ensures genome integrity by protecting and maintaining chromosome ends. Changes in its component…
View article: GERONIMO: A tool for systematic retrieval of structural RNAs in a broad evolutionary context
GERONIMO: A tool for systematic retrieval of structural RNAs in a broad evolutionary context Open
Background While web-based tools such as BLAST have made identifying conserved gene homologs appear easy, genes with variable sequences pose significant challenges. Functionally important noncoding RNAs (ncRNA) often show low sequence cons…
View article: Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis
Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis Open
In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA poly…
View article: Hymenoptera (Insecta) telomerase RNAs switched to plant/ciliate-like biogenesis
Hymenoptera (Insecta) telomerase RNAs switched to plant/ciliate-like biogenesis Open
In contrast to the catalytic subunit of telomerase, its RNA subunit (TR) is highly divergent in size, sequence and biogenesis pathways across eukaryotes. Current views on TR evolution assume a common origin of TRs transcribed with RNA poly…
View article: Perspectives - Decoding of novel selenoprotein in Homo sapiens
Perspectives - Decoding of novel selenoprotein in Homo sapiens Open
Using Polysome-seq, we identified active translation of previously known human snRNA (SNORD2402_22) which is lacking any obvious open reading frame (ORF). Using Selenocysteine pulldown experiment and subsequent RNAseq, we demonstrated inco…
View article: Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae Open
Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogen…
View article: Evolution of plant telomerase RNAs: farther to the past, deeper to the roots
Evolution of plant telomerase RNAs: farther to the past, deeper to the roots Open
The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by R…
View article: Origin and Fates of TERT Gene Copies in Polyploid Plants
Origin and Fates of TERT Gene Copies in Polyploid Plants Open
The gene coding for the telomerase reverse transcriptase (TERT) is essential for the maintenance of telomeres. Previously we described the presence of three TERT paralogs in the allotetraploid plant Nicotiana tabacum, while a single TERT c…
View article: Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences
Human-like telomeres in Zostera marina reveal a mode of transition from the plant to the human telomeric sequences Open
A previous study describing the genome of Zostera marina, the most widespread seagrass in the Northern hemisphere, revealed some genomic signatures of adaptation to the aquatic environment such as the loss of stomatal genes, while other fu…
View article: Human-like telomeres in <i>Zostera marina</i> reveal a mode of transition from the plant to the human telomeric sequences
Human-like telomeres in <i>Zostera marina</i> reveal a mode of transition from the plant to the human telomeric sequences Open
A previous study describing the genome of Zostera marina , the most widespread seagrass in the Northern hemisphere, revealed some genomic signatures of adaptation to the aquatic environment. Important features related to the ‘back-to-the-s…
View article: Telomerase RNAs in land plants
Telomerase RNAs in land plants Open
To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt)…
View article: <i>Allium</i> telomeres unmasked: the unusual telomeric sequence (<scp>CTCGGTTATGGG</scp>)<sub><i>n</i></sub> is synthesized by telomerase
<i>Allium</i> telomeres unmasked: the unusual telomeric sequence (<span>CTCGGTTATGGG</span>)<sub><i>n</i></sub> is synthesized by telomerase Open
Summary Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, r…
View article: Characterisation of an unusual telomere motif (<scp>TTTTTTAGGG</scp>)<sub>n</sub> in the plant <i>Cestrum elegans</i> (Solanaceae), a species with a large genome
Characterisation of an unusual telomere motif (<span>TTTTTTAGGG</span>)<sub>n</sub> in the plant <i>Cestrum elegans</i> (Solanaceae), a species with a large genome Open
Summary The characterization of unusual telomere sequence sheds light on patterns of telomere evolution, maintenance and function. Plant species from the closely related genera Cestrum, Vestia and Sessea (family Solanaceae) lack known plan…