Petr Králík
YOU?
Author Swipe
View article: Novel and simple qPCR system for the detection and quantification of the probiotic bacteria in food supplements
Novel and simple qPCR system for the detection and quantification of the probiotic bacteria in food supplements Open
The food supplements gain an attractivity among consumers due to their pronounced health benefits. There is no legal frame for probiotic food supplements and the only controllable aspect is the agreement of the declaration with the real co…
View article: Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection
Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection Open
View article: Six genetically linked mutations in the CD36 gene significantly delay the onset of Alzheimer's disease
Six genetically linked mutations in the CD36 gene significantly delay the onset of Alzheimer's disease Open
The risk of Alzheimer’s disease (AD) has a strong genetic component, also in the case of late-onset AD (LOAD). Attempts to sequence whole genome in large populations of subjects have identified only a few mutations common to most of the pa…
View article: A viability assay combining palladium compound treatment with quantitative PCR to detect viable Mycobacterium avium subsp. paratuberculosis cells
A viability assay combining palladium compound treatment with quantitative PCR to detect viable Mycobacterium avium subsp. paratuberculosis cells Open
View article: A Novel Approach to the Viability Determination of Mycobacterium avium subsp. paratuberculosis Using Platinum Compounds in Combination With Quantitative PCR
A Novel Approach to the Viability Determination of Mycobacterium avium subsp. paratuberculosis Using Platinum Compounds in Combination With Quantitative PCR Open
Mycobacterium avium subsp. paratuberculosis (MAP) represents a slow-growing bacterium causing paratuberculosis, especially in domestic and wild ruminants. Until recently, the assessment of MAP viability relied mainly on cultivation, which …
View article: Occurrence of SARS-CoV-2 in Indoor Environments With Increased Circulation and Gathering of People
Occurrence of SARS-CoV-2 in Indoor Environments With Increased Circulation and Gathering of People Open
At the time of sampling (2020/2021), the number of new cases of SARS-CoV-2-positive individuals in the Czech Republic significantly exceeded the numbers in neighboring countries and in the EU. In terms of the number of deaths, the country …
View article: Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR
Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR Open
View article: MOL-PCR and xMAP Technology
MOL-PCR and xMAP Technology Open
Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements …
View article: Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR Assay
Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR Assay Open
Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. For this reason, many instrument platforms need to be introd…
View article: Efficiency of DNA Isolation Methods Based on Silica Columns and Magnetic Separation Tested for the Detection of Mycobacterium avium Subsp. Paratuberculosis in Milk and Faeces
Efficiency of DNA Isolation Methods Based on Silica Columns and Magnetic Separation Tested for the Detection of Mycobacterium avium Subsp. Paratuberculosis in Milk and Faeces Open
Timely and reliable detection of animals shedding Mycobacterium avium subsp. paratuberculosis (MAP) should help to effectively identify infected animals and limit infection transmission at early stages to ensure effective control of paratu…
View article: Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR
Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR Open
Quantitative PCR (qPCR) is a widely used method for nucleic acid quantification of various pathogenic microorganisms. For absolute quantification of microbial load by qPCR, it is essential to create a calibration curve from accurately quan…
View article: Monitoring of selected parasites and other foodborne microorganisms in vegetable products at point of sale
Monitoring of selected parasites and other foodborne microorganisms in vegetable products at point of sale Open
The authors have withdrawn this preprint due to author disagreement.
View article: Monitoring of selected parasites and other foodborne microorganisms in vegetable products at point of sale
Monitoring of selected parasites and other foodborne microorganisms in vegetable products at point of sale Open
It is supposed that epidemiological data linked to cases of alimentary infection from fresh produce are scarce and usually underestimated. The present study reveals data concerning the occurrence of less studied foodborne pathogens such as…
View article: Control of paratuberculosis: who, why and how. A review of 48 countries
Control of paratuberculosis: who, why and how. A review of 48 countries Open
View article: Author Correction: One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles
Author Correction: One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles Open
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
View article: Author Correction: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
Author Correction: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay Open
View article: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay Open
View article: The Impact of the Antimicrobial Compounds Produced by Lactic Acid Bacteria on the Growth Performance of Mycobacterium avium subsp. paratuberculosis
The Impact of the Antimicrobial Compounds Produced by Lactic Acid Bacteria on the Growth Performance of Mycobacterium avium subsp. paratuberculosis Open
Cell-free supernatants (CFSs) extracted from various lactic acid bacteria (LAB) cultures were applied to Mycobacterium avium subsp. paratuberculosis (MAP) cells to determine their effect on MAP viability. In addition, 5% lact…
View article: One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles
One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles Open
MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it…
View article: Detection and Quantification of <i>Listeria</i><i> </i><i>m</i><i>onocytogenes</i> in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR
Detection and Quantification of <i>Listeria</i><i> </i><i>m</i><i>onocytogenes</i> in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR Open
In this study, a total of 175 samples of ready-to-eat vegetables, frozen vegetables and sprouted seeds originating in 10 states of the European Union and from 32 manufacturers were collected during a period of one year and examined for the…
View article: A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything Open
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept o…
View article: xMAP Technology: Applications in Detection of Pathogens
xMAP Technology: Applications in Detection of Pathogens Open
xMAP technology is applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, en…
View article: Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices
Preparation of MS2 Phage-Like Particles and Their Use As Potential Process Control Viruses for Detection and Quantification of Enteric RNA Viruses in Different Matrices Open
The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). To control the whole process of analysis and in …
View article: Changes in the Expression of Biofilm-Associated Surface Proteins in<i>Staphylococcus aureus</i>Food-Environmental Isolates Subjected to Sublethal Concentrations of Disinfectants
Changes in the Expression of Biofilm-Associated Surface Proteins in<i>Staphylococcus aureus</i>Food-Environmental Isolates Subjected to Sublethal Concentrations of Disinfectants Open
Sublethal concentrations (sub-MICs) of certain disinfectants are no longer effective in removing biofilms from abiotic surfaces and can even promote the formation of biofilms. Bacterial cells can probably adapt to these low concentrations …
View article: Impaired Albumin Uptake and Processing Promote Albuminuria in OVE26 Diabetic Mice
Impaired Albumin Uptake and Processing Promote Albuminuria in OVE26 Diabetic Mice Open
The importance of proximal tubules dysfunction to diabetic albuminuria is uncertain. OVE26 mice have the most severe albuminuria of all diabetic mouse models but it is not known if impaired tubule uptake and processing are contributing fac…
View article: Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens
Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens Open