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View article: SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity
SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity Open
View article: Avian interferon regulatory factor (IRF) family reunion: IRF3 and IRF9 found
Avian interferon regulatory factor (IRF) family reunion: IRF3 and IRF9 found Open
View article: P4PP: A Universal Shotgun Proteomics Data Analysis Pipeline for Virus Identification
P4PP: A Universal Shotgun Proteomics Data Analysis Pipeline for Virus Identification Open
Humans can be infected by a wide variety of virus species. We developed a data analysis approach for shotgun proteomic data to detect these viruses. A proteome for pandemic preparedness (P4PP) pipeline, a corresponding database (P4PP v01),…
View article: Sex Chromosome Turnover and Structural Interspecific Genome Divergence Shapes Meiotic Outcomes in Hybridizing <i>Cobitis</i>
Sex Chromosome Turnover and Structural Interspecific Genome Divergence Shapes Meiotic Outcomes in Hybridizing <i>Cobitis</i> Open
It has been empirically established that genome mixing between divergent species can trigger meiotic aberrations, ultimately leading to the emergence of asexual reproduction through the production of unreduced gametes in various metazoan l…
View article: Differential effect of monoterpenes and flavonoids on the transcription of aromatic ring-hydroxylating dioxygenase genes in Rhodococcus opacus C1 and Rhodococcus sp. WAY2
Differential effect of monoterpenes and flavonoids on the transcription of aromatic ring-hydroxylating dioxygenase genes in Rhodococcus opacus C1 and Rhodococcus sp. WAY2 Open
Aromatic ring-hydroxylating dioxygenases (ARHDs) play a crucial role in the aerobic biodegradation of both natural and anthropogenic aromatic compounds. Although their ability to process contaminants is not entirely understood, it is thoug…
View article: Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds
Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds Open
Bartonellae are zoonotic pathogens with a broad range of reservoir hosts and vectors. To examine sylvatic Bartonella reservoirs, tissue samples of red deer (Cervus elaphus, n = 114) and their associated deer keds (Lipoptena cervi, n = 50; …
View article: An integrated microfluidic platform for nucleic acid testing
An integrated microfluidic platform for nucleic acid testing Open
This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 diagnostics. The system integrates the extraction and purification of nucleic acids, followed by amplification via either reverse transcription-quant…
View article: Multiple and frequent trypanosomatid co-infections of insects: the Cuban case study
Multiple and frequent trypanosomatid co-infections of insects: the Cuban case study Open
Trypanosomatids are obligate parasites of animals, predominantly insects and vertebrates, and flowering plants. Monoxenous species, representing the vast majority of trypanosomatid diversity, develop in a single host, whereas dixenous spec…
View article: Proteogenomic Characterization of Pseudomonas veronii SM-20 Growing on Phenanthrene as Only Carbon and Energy Source
Proteogenomic Characterization of Pseudomonas veronii SM-20 Growing on Phenanthrene as Only Carbon and Energy Source Open
In this study, we conducted an extensive investigation of the biodegradation capabilities and stress response of the newly isolated strain Pseudomonas veronii SM-20 in order, to assess its potential for bioremediation of sites contaminated…
View article: RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria
RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria Open
Bacteria have evolved structured RNAs that can associate with RNA polymerase (RNAP). Two of them have been known so far—6S RNA and Ms1 RNA but it is unclear if any other types of RNAs binding to RNAP exist in bacteria. To identify all RNAs…
View article: Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects
Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects Open
View article: Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes
Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes Open
Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in r…
View article: Rapid gene content turnover on the germline-restricted chromosome in songbirds
Rapid gene content turnover on the germline-restricted chromosome in songbirds Open
View article: Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma Open
Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
View article: Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells Open
Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
View article: Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells Open
Supplementary Materials from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
View article: Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma Open
Supplementary Table 1 from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
View article: Data from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
Data from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma Open
Gene deregulation is a frequent cause of malignant transformation. Alteration of the gene structure and/or expression leading to cellular transformation and tumor growth can be experimentally achieved by insertion of the retroviral genome …
View article: Data from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
Data from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells Open
A tumor cell is formed when a critical amount of endogenous and/or exogenous tumorigenic stimuli is exceeded. We have shown that the transient presence of nontumorigenic stray cells in tissues of experimental animals that contain cells wit…
View article: Data from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
Data from Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells Open
A tumor cell is formed when a critical amount of endogenous and/or exogenous tumorigenic stimuli is exceeded. We have shown that the transient presence of nontumorigenic stray cells in tissues of experimental animals that contain cells wit…
View article: Data from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma
Data from Identification of Potential Human Oncogenes by Mapping the Common Viral Integration Sites in Avian Nephroblastoma Open
Gene deregulation is a frequent cause of malignant transformation. Alteration of the gene structure and/or expression leading to cellular transformation and tumor growth can be experimentally achieved by insertion of the retroviral genome …
View article: Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis
Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis Open
View article: Culturomics of Bacteria from Radon-Saturated Water of the World’s Oldest Radium Mine
Culturomics of Bacteria from Radon-Saturated Water of the World’s Oldest Radium Mine Open
The mine Svornost in Jáchymov (Joachimstahl), Czech Republic is a former silver-uranium mine and the world’s first and for a long time only radium mine, nowadays the deepest mine devoted to the extraction of water which is saturated with r…
View article: Retrospective Analysis Revealed an April Occurrence of Monkeypox in the Czech Republic
Retrospective Analysis Revealed an April Occurrence of Monkeypox in the Czech Republic Open
Herein, we present our findings of an early appearance of the Monkeypox virus in Prague, Czech Republic. A retrospective analysis of biological samples, carried out on the 28th of April, revealed a previously unrecognized case of Monkeypox…
View article: Correction to: The first case of Planococcus glaciei found in blood, a report from the Czech Republic
Correction to: The first case of Planococcus glaciei found in blood, a report from the Czech Republic Open
View article: Clinically silent LINE 1 insertion in the PNPLA3 gene may impede genotyping of the p.I148M variant
Clinically silent LINE 1 insertion in the PNPLA3 gene may impede genotyping of the p.I148M variant Open
View article: Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond Open
Currently, the ongoing pandemic has created an unprecedented pressure for the development of various analytical assays and especially their immediate practical applications. A number of tests based on different principles such as immunoass…
View article: Salmonella Paratyphi Infection: Use of Nanopore Sequencing as a Vivid Alternative for the Identification of Invading Bacteria
Salmonella Paratyphi Infection: Use of Nanopore Sequencing as a Vivid Alternative for the Identification of Invading Bacteria Open
In our study we present an overview of the use of Oxford Nanopore Technologies (ONT) sequencing technology on the background of Enteric fever. Unlike traditional methods (e.g., qPCR, serological tests), the nanopore sequencing technology e…
View article: Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR Assay
Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR Assay Open
Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. For this reason, many instrument platforms need to be introd…
View article: Exosomes released by imatinib‑resistant K562 cells contain specific membrane markers, IFITM3, CD146 and CD36 and increase the survival of imatinib‑sensitive cells in the presence of imatinib
Exosomes released by imatinib‑resistant K562 cells contain specific membrane markers, IFITM3, CD146 and CD36 and increase the survival of imatinib‑sensitive cells in the presence of imatinib Open
Chronic myeloid leukemia (CML) is a malignant hematopoietic disorder distinguished by the presence of a BCR‑ABL1 fused oncogene with constitutive kinase activity. Targeted CML therapy by specific tyrosine kinase inhibitors (TKIs) leads to …