Explanipedia

sRNA_seq_clean_thrips_leafdiscs_timeseries Open

Marlot Westera, Petra Bleeker · 2039

2023-03 Timeseries experiment with clean thrips on leafdiscs of tomato MoneyMaker plants. srna and mrna were extracted with the kit 'NucleoSpin miRNA, Mini kit for miRNA and RNA purification'.\ncontains (see metadata file for more …

sRNA_seq_clean_thrips_leafdiscs_timeseries Open

Marlot Westera, Petra Bleeker · 2039

2023-03 Timeseries experiment with clean thrips on leafdiscs of tomato MoneyMaker plants. srna and mrna were extracted with the kit 'NucleoSpin miRNA, Mini kit for miRNA and RNA purification'. contains (see metadata file for more info): - …

A phloem‐based defense mechanism linked to elevated riboflavin levels in wild tomato Solanum chmielewskii impedes whitefly nymphal development Open

Lissy‐Anne M. Denkers, Arjen van Doorn, Marc Galland, Gerd U. Balcke, Martin de Vos , et al. · 2025

SUMMARY Management of the phloem‐feeding pest insect Bemisia tabaci (whitefly) is difficult due to its short generation time and large number of offspring. Several whitefly‐resistant wild tomato accessions have been identified, with the re…

Glandular trichome rupture in tomato plants is an ultra-fast and sensitive defense mechanism against insects Open

J Popowski, Lucas D. Warma, Alicia Abarca, Petra Bleeker, Maziyar Jalaal · 2025

Trichomes, specialized hair-like structures on the surfaces of many plants, play a crucial role in defense against herbivorous insects. We investigated the biomechanics of type VI glandular trichome rupture in cultivated tomato (Solanum ly…

Glandular Trichome Rupture in Tomato Plants is an Ultra-Fast & Sensitive Defense Mechanism Against Insects Open

J Popowski, Lucas Warma, Alejandro Cifuentes, Petra Bleeker, Maziyar Jalaal · 2024

Trichomes, specialized hair-like structures on the surfaces of many plants, play a crucial role in defense against herbivorous insects. We investigated the biomechanics of type VI glandular trichomes in cultivated tomato ( Solanum lycopers…

Glandular Trichome Rupture in Tomato Plants is an Ultra-Fast & Sensitive Defense Mechanism Against Insects Open

J Popowski, Lucas Warma, Alejandro Cifuentes, Petra Bleeker, Maziyar Jalaal · 2024

Trichomes, specialized hair-like structures on the surfaces of many plants, play a crucial role in defense against herbivorous insects. We investigated the biomechanics of type VI glandular trichomes in cultivated tomato (Solanum lycopersi…

PhenoFeatureFinder: a python package for linkingdevelopmental phenotypes to omics features Open

Lissy‐Anne M. Denkers, Marc Galland, A. Dekker, Valerio Bianchi, Petra Bleeker · 2024

International audience

Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato Open

Eloisa Vendemiatti, Inty Omar Hernández-De Lira, Roxane Snijders, Tanmayee Torne, Rodrigo Therezan , et al. · 2023

Type-IV glandular trichomes, which only occur in the juvenile phase of tomato development, produce acylsugars (AS) that broadly protect against arthropod herbivory. Previously, we introgressed the capacity to retain type-IV trichomes in th…

Genetic and physiological requirements for high-level sesquiterpene-production in tomato glandular trichomes Open

Ruy Kortbeek, Marc Galland, Aleksandra Muras, Rodrigo Therezan, Sofia Maia , et al. · 2023

Type-VI glandular trichomes of wild tomato Solanum habrochaites PI127826 produce high levels of the sesquiterpene 7-epizingiberene and its derivatives, making the plant repellent and toxic to several pest insects and pathogens. How wild to…

Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach Open

Ruy Kortbeek, Marc Galland, Aleksandra Muras, Frans van der Kloet, Bart André , et al. · 2021

Introgression of the sesquiterpene biosynthesis from Solanum habrochaites to cultivated tomato offers insights into trichome morphology and arthropod resistance Open

Rodrigo Therezan, Ruy Kortbeek, Eloisa Vendemiatti, Saioa Legarrea, Severino Matias de Alencar , et al. · 2021

Main conclusion Cultivated tomatoes harboring the plastid-derived sesquiterpenes from S. habrochaites have altered type-VI trichome morphology and unveil additional genetic components necessary for piercing-sucking pest resistance. Abstrac…

LTP2 related material Open

Marc Galland, Rodrigo Therezan, Ruy Kortbeek, Petra Bleeker · 2021

Messenger RNA-seq data of tomato lines affected in the LTP2 gene. The sample list can be found in list_of_samples.csv.

LTP2 related material Open

Marc Galland, Rodrigo Therezan, Ruy Kortbeek, Petra Bleeker · 2021

Messenger RNA-seq data of tomato lines affected in the LTP2 gene. RNA isolation To isolate RNA, plant tissues were flash frozen in liquid nitrogen immediately after harvesting and stored at −80°C prior to …

LTP2 related material Open

Marc Galland, Rodrigo Therezan, Ruy Kortbeek, Petra Bleeker · 2021

Messenger RNA-seq data of tomato lines affected in the LTP2 gene. RNA isolation To isolate RNA, plant tissues were flash frozen in liquid nitrogen immediately after harvesting and stored at −80°C prior to …

LTP2 related material Open

Marc Galland, Rodrigo Therezan, Ruy Kortbeek, Petra Bleeker · 2021

Messenger RNA-seq data of tomato lines affected in the LTP2 gene. RNA isolation To isolate RNA, plant tissues were flash frozen in liquid nitrogen immediately after harvesting and stored at −80°C prior t…

Additional file 11 of Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach Open

Ruy Kortbeek, Marc Galland, Aleksandra Muras, Frans van der Kloet, Bart André , et al. · 2021

Additional file 11: Table S3. Normalised peak-area of the volatiles and acylsugars detected over the samples. The file contains the data that was used as input material for Fig. 3, Fig. 5 and the random forest models and includes the exper…

Additional file 9 of Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach Open

Ruy Kortbeek, Marc Galland, Aleksandra Muras, Frans van der Kloet, Bart André , et al. · 2021

Additional file 9: Table S1. Generalised Linear Model fitted on the whitefly survival data. The output of the model was used to classify accessions as either resistant or susceptible to whiteflies which served as an input for the random fo…

Additional file 10 of Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach Open

Ruy Kortbeek, Marc Galland, Aleksandra Muras, Frans van der Kloet, Bart André , et al. · 2021

Additional file 10: Table S2. Cox proportional hazard model fitted on the thrips survival data. The output of the model was used to classify accessions as either resistant or susceptible to whiteflies which served as an input for the rando…

A single cytochrome P450 oxidase from Solanum habrochaites sequentially oxidizes 7‐epi‐zingiberene to derivatives toxic to whiteflies and various microorganisms Open

Sebastian Zabel, Wolfgang Brandt, Andrea Porzel, Benedikt Athmer, Stefan Bennewitz , et al. · 2020

Summary Secretions from glandular trichomes potentially protect plants against a variety of aggressors. In the tomato clade of the Solanum genus, glandular trichomes of wild species produce a rich source of chemical diversity at the leaf s…

Processed data for the sRNA landscape chapter Open

Marc Galland, Michelle Van Der Gragt, Petra Bleeker · 2020

Processed data to be used in analyses related to the sRNA landscape. 1) small RNA processed data from stem trichomes: 2020-12-17_11-23_results_stem_trichomes.tar.gz Original small RNA-seq fastq files: ava…

Processed data for the sRNA landscape chapter Open

Marc Galland, Michelle Van Der Gragt, Petra Bleeker · 2020

Processed data to be used in analyses related to the sRNA landscape. 1) small RNA processed data from stem trichomes: 2020-12-03_results_small_rna_stem_trichomes.tar.gz Original small RNA-seq fastq files:…

DNA-seq of Solanum habrochaites PI127826 and LA1777 DNA (part II) Open

Petra Bleeker, Petra Bleeker · 2020

Illumina paired-end HiSeqX reads from the genomic DNA isolated from young leaves of three plants from S. habrochaites: Solanum habrochaites PI127826 Solanum habrochaites LA1777 (wild-type) Solanum habrochaites LA1777 X-ray mutated…

DNA-seq of Solanum habrochaites PI127826 and LA1777 DNA (part II) Open