Petra Hellwig
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View article: Computational study of heme <i>b</i> <sub>595</sub> to heme <i>d</i> electron transfer in E. coli cytochrome <i>bd</i>-I oxidase
Computational study of heme <i>b</i> <sub>595</sub> to heme <i>d</i> electron transfer in E. coli cytochrome <i>bd</i>-I oxidase Open
Cytochrome bd is a distinctive family of terminal oxidases present in the respiratory chains of many prokaryotes. Despite its biological importance, the redox chemistry of these proteins remains poorly understood, largely due to the presen…
Resonance Raman Spectroscopic Study of the Unusual [4Fe‐4S]<sup>2+</sup> Cluster of IspH, the Last Enzyme of the Methylerythritol Phosphate Pathway for Terpenoid Biosynthesis Open
IspH is the last enzyme of the methylerythritol phosphate pathway. It catalyzes the reductive dehydroxylation of ( E )‐4‐hydroxy‐3‐methyl‐but‐2‐en‐1‐yl diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), w…
Mild and Selective Trifluoromethylation of Peptides at Tryptophane Residues by a Water‐Soluble Copper Complex with Redox‐Active Ligands Open
Selective chemical modifications of biomolecules are in high demand for the development and implementation of chemical biology strategies. Methods involving radicals have emerged as powerful options yet potentially generate side reactiviti…
Graphene Nanodots as Substrates for SEIRAS and SERS Studies on Membrane Proteins Open
Graphene nanostructures are capable of supporting plasmonic resonances in the visible and infrared parts of the spectrum. Thus, they can be exploited as platforms for Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) and Surface-E…
The electrochemical properties of the highly diverse terminal oxidases from different organisms Open
Terminal oxidases are critical for aerobic respiratory chains of prokaryotes and eukaryotes, responsible for the final step in the electron transport chain. These enzymes catalyze the transfer of electrons from reduced electron carriers (s…
Interplay of acidic residues in the proton channel of E. coli cytochrome bd-I oxidase to promote oxygen reduction and NO release Open
The reduction of oxygen to water is crucial to life under aerobic conditions. Cytochrome bd oxidases perform this reaction with a very high oxygen affinity. Members of this protein family are solely found in prokaryotes and some archaea pl…
Spectroscopic evidence of the interaction of titanium(<span>iv</span>) coordination complexes with a phosphate head group in phospholipids Open
Multiple spectroscopic techniques permit the assignment of interaction existing between Ti( iv ) catecholato-based coordination compounds and phospholipids.
View article: Towards Bacterial Resistance <i>via</i> the Membrane Strategy: Enzymatic, Biophysical and Biomimetic Studies of the Lipid <i>cis</i>‐<i>trans</i> Isomerase of <i>Pseudomonas aeruginosa</i>
Towards Bacterial Resistance <i>via</i> the Membrane Strategy: Enzymatic, Biophysical and Biomimetic Studies of the Lipid <i>cis</i>‐<i>trans</i> Isomerase of <i>Pseudomonas aeruginosa</i> Open
The lipid cis ‐ trans isomerase (Cti) is a periplasmic heme‐ c enzyme found in several bacteria including Pseudomonas aeruginosa , a pathogen known for causing nosocomial infections. This metalloenzyme catalyzes the cis ‐ trans isomerizati…
The small membrane protein CcoS is involved in cofactor insertion into the cbb3-type cytochrome c oxidase Open
Respiratory complexes, such as cytochrome oxidases, are cofactor-containing multi-subunit protein complexes that are critically important for energy metabolism in all domains of life. Their intricate assembly strictly depends on accessory …
Assessment of the concomitant action of XBD173 and interferon β in a mouse model of multiple sclerosis using infrared marker bands Open
Disease modifying therapies including interferon-β (IFNβ) effectively counteract the inflammatory component in relapsing-remitting multiple sclerosis (RRMS) but this action, generally associated with severe side effects, does not prevent a…
View article: Quinone chemistry in respiratory complex I involves protonation of a conserved aspartic acid residue
Quinone chemistry in respiratory complex I involves protonation of a conserved aspartic acid residue Open
Respiratory complex I is a central metabolic enzyme coupling NADH oxidation and quinone reduction with proton translocation. Despite the knowledge of the structure of the complex, the coupling of both processes is not entirely understood. …
Quest for a stable Cu-ligand complex with a high catalytic activity to produce reactive oxygen species Open
Metal ion–catalyzed overproduction of reactive oxygen species (ROS) is believed to contribute significantly to oxidative stress and be involved in several biological processes, from immune defense to development of diseases. Among the esse…
Editorial: Computational and experimental insights in proton and ion translocating bioenergetic systems Open
EDITORIAL article Front. Chem., 05 March 2024Sec. Theoretical and Computational Chemistry Volume 12 - 2024 | https://doi.org/10.3389/fchem.2024.1384385
View article: Copper-Catalyzed Glutathione Oxidation is Accelerated by the Anticancer Thiosemicarbazone Dp44mT and Further Boosted at Lower pH
Copper-Catalyzed Glutathione Oxidation is Accelerated by the Anticancer Thiosemicarbazone Dp44mT and Further Boosted at Lower pH Open
Glutathione (GSH) is the most abundant thiol in mammalian cells and plays a crucial role in maintaining redox cellular homeostasis. The thiols of two GSH molecules can be oxidized to the disulfide GSSG. The cytosolic GSH/GSSG ratio is very…
Copper-catalysed glutathione oxidation is accelerated by the anticancer thiosemicarbazone Dp44mT and further boosted at lower pH Open
Glutathione (GSH) is the most abundant thiol in mammalian cells and plays a crucial role in maintaining the redox state of many biomolecules, in detoxification and in antioxidant defence. The thiols of two GSH molecules can be oxidized to …
Copper-catalysed glutathione oxidation is accelerated by the anticancer thiosemicarbazone Dp44mT and further boosted at lower pH Open
Glutathione (GSH) is the most abundant thiol in mammalian cells and plays a crucial role in maintaining the redox state of many biomolecules, in detoxification and in antioxidant defence. The thiols of two GSH molecules can be oxidized to …
Rapid Discrimination of Neuromyelitis Optica Spectrum Disorder and Multiple Sclerosis Using Machine Learning on Infrared Spectra of Sera Open
Neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) are both autoimmune inflammatory and demyelinating diseases of the central nervous system. NMOSD is a highly disabling disease and rapid introduction of the appropr…
An accurate vibrational signature in halogen bonded molecular crystals Open
A unique fingerprint of the halogen bond in two halogen bonded networks has been evidenced in the low frequency region, using both far infrared and Raman spectroscopy, together with DFT calculations.
Plasmonic Resonant Nanoantennas Induce Changes in the Shape and the Intensity of Infrared Spectra of Phospholipids Open
Surface enhanced infrared absorption spectroscopic studies (SEIRAS) as a technique to study biological molecules in extremely low concentrations is greatly evolving. In order to use the technique for identification of the structure and int…
Editorial: Computational and Experimental Insights in Redox-Coupled Proton Pumping in Proteins Open
EDITORIAL article Front. Chem., 15 September 2021 | https://doi.org/10.3389/fchem.2021.762612
View article: Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology
Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology Open
Surface-enhanced infrared absorption spectroscopy (SEIRAS) is a powerful tool that allows studying the reactivity of protein monolayers at very low concentrations and independent from the protein size. In this study, we probe the surface's…
The Inhibition Study of Cytochrome bd Oxidase Using the Enzyme-Based Electrochemical Sensor Open
Membrane proteins that participate in multiple vital functions of every living organism such as transport, signaling and respiration, provide 80 to 90% of the relevant targets for the pharmaceutical industries. The family of cytochrome bd …
A hybrid bioinspired catechol-alloxazine triangular nickel complex stabilizing protons and electrons Open
A new class of redox-active ligands merging catechol and alloxazine structures is reported. A trimetallic triangular complex is formed upon complexation to nickel.
Asp22 drives the protonation state of the Staphylococcus epidermidis glucose/H+ symporter Open
The Staphylococcus epidermidis glucose/H+ symporter (GlcPSe) is a membrane transporter highly specific for glucose and a homolog of the human glucose transporters (GLUT, SLC2 family). Most GLUTs and their bacterial counterparts differ in t…
Monoclonal antibody 4B1 influences the p<i>K</i><sub>a</sub> of Glu325 in lactose permease (LacY) from <i>Escherichia coli</i>: evidence from SEIRAS Open
The monoclonal antibody 4B1 binds to a conformational epitope on the periplasmic side of lactose permease (LacY) of Escherichia coli and inhibits H + /lactose symport and lactose efflux under nonenergized conditions. At the same time, liga…