Philipp Lewe
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View article: Disassembly of the <i>Escherichia coli</i> AcrABZ-TolC efflux pump by ligand-mediated disruption of TolC-AcrA interfacial contacts
Disassembly of the <i>Escherichia coli</i> AcrABZ-TolC efflux pump by ligand-mediated disruption of TolC-AcrA interfacial contacts Open
The outer membrane factor TolC is an essential component of various efflux pump complexes in E. coli and represents a potential target for antibiotic adjuvants. By means of a virtual screen for TolC-binding compounds, we identified the kin…
Efflux Pump-Binding 4(3-Aminocyclobutyl)Pyrimidin-2-Amines Are Colloidal Aggregators Open
Efflux pumps are a relevant factor in antimicrobial resistance. In E. coli, the tripartite efflux pump AcrAB-TolC removes a chemically diverse set of antibiotics from the bacterium. Therefore, small molecules interfering with efflux pump f…
View article: Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins
Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins Open
Successful sample preparation is the foundation to any structural biology technique. Membrane proteins are of particular interest as these are important targets for drug design, but also notoriously difficult to work with. For electron cry…
Signaling ammonium across membranes through an ammonium sensor histidine kinase Open
Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing (anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium transporters. Here, we report on the structure and function of an ammon…