Explanipedia

Single-molecule FRET and tracking of transfected biomolecules in living cells Open

Abhinaya Anandamurugan, Antonia Eidloth, Veronika Frank, Philipp Wortmann, Lukas Schrangl , et al. · 2025

Proteins and DNA in cells exhibit different conformational states, which are influenced by dynamic interactions with other biomolecules. All these interactions are affected by the molecules' localization within the cell, i.e., their compar…

Auxiliary CycleGAN-guidance for Task-Aware Domain Translation from Duplex to Monoplex IHC Images Open

Nicolas Brieu, Nicolas Triltsch, Philipp Wortmann, Dominik Winter, Shashank Saran , et al. · 2024

Generative models enable the translation from a source image domain where readily trained models are available to a target domain unseen during training. While Cycle Generative Adversarial Networks (GANs) are well established, the associat…

Single-Molecule FRET and Tracking of Transfected Biomolecules in Living Cells Open

Abhinaya Anandamurugan, Antonia Eidloth, Philipp Wortmann, Lukas Schrangl, Fernando Aprile-Garcia , et al. · 2023

Proteins and DNA in cells exhibit different conformational states, which are influenced by dynamic interactions with other biomolecules. All these interactions are affected by the molecules’ localization within the cell, i.e., their compar…

339 Quantitative assessment of CAR-T cell therapy targets using computational pathology Open

Nathalie Harder, Markus Schick, Pallavi Sontakke, Alma Andoni, Karma Dacosta , et al. · 2022

Background CAR-T cell-based therapies have improved the treatment of some advanced lymphomas and leukemias. While loss of CAR-T target expression is an established resistance mechanism, a large fraction of target-negative patients responde…

Stain Isolation-based Guidance for Improved Stain Translation Open

Nicolas Brieu, Felix J. Segerer, Ansh Kapil, Philipp Wortmann, Günter Schmidt · 2022

Unsupervised and unpaired domain translation using generative adversarial neural networks, and more precisely CycleGAN, is state of the art for the stain translation of histopathology images. It often, however, suffers from the presence of…

Protein Conformational Dynamics In Cellula and IN VIVO Using Live-Cell Single-Molecule Fret Hilo Microscopy Open

Abhinaya Anandamurugan, Philipp Wortmann, Chenyang Lan, April Garcia Fernando, Marina Veil , et al. · 2021

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions Open

Markus Götz, Philipp Wortmann, Sonja Schmid, Thorsten Hugel · 2018

Single-molecule Förster resonance energy transfer (smFRET) has become a widely used biophysical technique to study the dynamics of biomolecules. For many molecular machines in a cell proteins have to act together with interaction partners …

Cooperative Nucleotide Binding in Hsp90 and Its Regulation by Aha1 Open

Philipp Wortmann, Markus Götz, Thorsten Hugel · 2017

Cooperative nucleotide binding in Hsp90 and the underlying mechanisms Open

Philipp Wortmann, Markus Goötz, Thorsten Hugel · 2017

The function of the molecular chaperone Hsp90 depends on large conformational changes, rearrangement of local motifs, as well as the binding and hydrolysis of ATP. The complexity of the Hsp90 system impedes the detailed investigation of th…

Determination of structural ensembles and correlated dynamics of a multi-domain protein Open

Bjoern Hellenkamp, Bjoern Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias , et al. · 2017

This protocol describes how structural ensembles and state-dependent local dynamics of a multi-domain protein can be determined.The hybrid approach combines static structures of sub-domains with selfconsistent FRET networks derived from of…

The dynamics of the Hsp90 machine Open

Philipp Wortmann · 2017

The presented research uses three different single molecule FRET approaches to assess the structure and the dynamics of Hsp90 and its complexes. The highly dynamic in-solution structure of Hsp90’s open conformation is resolved as a structu…

Multidomain structure and correlated dynamics determined by self-consistent FRET networks Open

Björn Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias, Thorsten Hugel · 2016

Structural Dynamics of Hsp90 Resolved by a Novel Multi-Pair FRET Approach Open

Bjoern Hellenkamp, Philipp Wortmann, Florian Kandzia, Martin Zacharias, Thorsten Hugel · 2016

Studying the Hsp90 Machinery in Living Cells by Single Molecule FRET Open

Philipp Wortmann, Fernando Aprile-Garcia, Ritwick Sawarkar, Thorsten Hugel · 2016

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