Philippe Barthe
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View article: <i>Zymoseptoria tritici</i> Effectors Structurally Related to Killer Proteins <scp>UmV</scp> ‐ <scp>KP4</scp> and <scp>UmV</scp> ‐ <scp>KP6</scp> Inhibit Fungal Growth, and Define Extended Protein Families in Fungi
<i>Zymoseptoria tritici</i> Effectors Structurally Related to Killer Proteins <span>UmV</span> ‐ <span>KP4</span> and <span>UmV</span> ‐ <span>KP6</span> Inhibit Fungal Growth, and Define Extended Protein Families in Fungi Open
Fungal effectors play crucial roles in plant infection. Despite low sequence identity, they were recently discovered to belong to families with similar three‐dimensional structures. In this study, we elucidated the structures of Zt‐NIP1 an…
View article: Structural and Functional Characterization of the 28 <scp>kDa</scp> Structured Core of <scp>BmSA1</scp> , the Major Surface Antigen of <i>Babesia Microti</i>
Structural and Functional Characterization of the 28 <span>kDa</span> Structured Core of <span>BmSA1</span> , the Major Surface Antigen of <i>Babesia Microti</i> Open
Babesiosis is a tick‐borne disease that poses a significant threat to animal health worldwide. In addition, climate change and the risk of human‐to‐human transmission through blood transfusion have made babesiosis an emerging disease in hu…
View article: Understanding the Relationship between Pressure and Temperature Unfolding of Proteins
Understanding the Relationship between Pressure and Temperature Unfolding of Proteins Open
Proteins unfold under different environmental insults, among which are heat, cold, high pressure, and chaotropic agents. Understanding the mechanisms that determine unfolding under each of these conditions is an important problem that dire…
View article: <i>Zymoseptoria tritici</i>effectors structurally related to killer proteins UmV-KP4 and UmV-KP6 are toxic to fungi, and define extended protein families in fungi
<i>Zymoseptoria tritici</i>effectors structurally related to killer proteins UmV-KP4 and UmV-KP6 are toxic to fungi, and define extended protein families in fungi Open
Fungal effectors play crucial roles in plant infection. Despite low sequence identity, effectors were recently classified into structural families. In this study, we have elucidated the structures of Zt-NIP1 and Mycgr3-91409 effectors of t…
View article: Understanding the physical determinants of pressure denaturation: Exploring the unfolding pathways of Yfh1 at different temperatures with High-Pressure NMR
Understanding the physical determinants of pressure denaturation: Exploring the unfolding pathways of Yfh1 at different temperatures with High-Pressure NMR Open
Proteins unfold under different environmental insults, among which are heat, cold, high pressure and chaotropic agents. Understanding the mechanisms that determine unfolding under each of these conditions is an important problem that direc…
View article: The structural landscape and diversity of Pyricularia oryzae MAX effectors revisited
The structural landscape and diversity of Pyricularia oryzae MAX effectors revisited Open
Magnaporthe AVRs and ToxB-like (MAX) effectors constitute a family of secreted virulence proteins in the fungus Pyricularia oryzae (syn . Magnaporthe oryzae) , which causes blast disease on numerous cereals and grasses. In spite of high se…
View article: The structural landscape and diversity of<i>Pyricularia oryzae</i>MAX effectors revisited
The structural landscape and diversity of<i>Pyricularia oryzae</i>MAX effectors revisited Open
Magnaporthe AVRs and ToxB-like (MAX) effectors constitute a family of secreted virulence proteins in the fungus Pyricularia oryzae (syn. Magnaporthe oryzae) , which causes blast disease on numerous cereals and grasses. In spite of high seq…
View article: Does a Similar 3D Structure Mean a Similar Folding Pathway? The Presence of a C-Terminal α-Helical Extension in the 3D Structure of MAX60 Drastically Changes the Folding Pathway Described for Other MAX-Effectors from Magnaporthe oryzae
Does a Similar 3D Structure Mean a Similar Folding Pathway? The Presence of a C-Terminal α-Helical Extension in the 3D Structure of MAX60 Drastically Changes the Folding Pathway Described for Other MAX-Effectors from Magnaporthe oryzae Open
Does a similar 3D structure mean a similar folding pathway? This question is particularly meaningful when it concerns proteins sharing a similar 3D structure, but low sequence identity or homology. MAX effectors secreted by the phytopathog…
View article: A Mycobacterium tuberculosis Effector Targets Mitochondrion, Controls Energy Metabolism, and Limits Cytochrome <i>c</i> Exit
A Mycobacterium tuberculosis Effector Targets Mitochondrion, Controls Energy Metabolism, and Limits Cytochrome <i>c</i> Exit Open
In this article, using a combination of techniques (bioinformatics, structural biology, and cell biology), we identified and characterized a new class of effectors present only in intracellular mycobacteria. These proteins specifically tar…
View article: Combining High-Pressure NMR and Geometrical Sampling to Obtain a Full Topological Description of Protein Folding Landscapes: Application to the Folding of Two MAX Effectors from Magnaporthe oryzae
Combining High-Pressure NMR and Geometrical Sampling to Obtain a Full Topological Description of Protein Folding Landscapes: Application to the Folding of Two MAX Effectors from Magnaporthe oryzae Open
Despite advances in experimental and computational methods, the mechanisms by which an unstructured polypeptide chain regains its unique three-dimensional structure remains one of the main puzzling questions in biology. Single-molecule tec…
View article: Comparative Assessment of NMR Probes for the Experimental Description of Protein Folding Pathways with High-Pressure NMR
Comparative Assessment of NMR Probes for the Experimental Description of Protein Folding Pathways with High-Pressure NMR Open
Multidimensional NMR intrinsically provides multiple probes that can be used for deciphering the folding pathways of proteins: NH amide and CαHα groups are strategically located on the backbone of the protein, while CH3 groups, on the side…
View article: Pressure and Chemical Unfolding of an α-Helical Bundle Protein: The GH2 Domain of the Protein Adaptor GIPC1
Pressure and Chemical Unfolding of an α-Helical Bundle Protein: The GH2 Domain of the Protein Adaptor GIPC1 Open
When combined with NMR spectroscopy, high hydrostatic pressure is an alternative perturbation method used to destabilize globular proteins that has proven to be particularly well suited for exploring the unfolding energy landscape of small…
View article: A<i>Mycobacterium tuberculosis</i>effector targets mitochondrion, controls energy metabolism and limits cytochrome c exit
A<i>Mycobacterium tuberculosis</i>effector targets mitochondrion, controls energy metabolism and limits cytochrome c exit Open
Host metabolism reprogramming is a key feature of Mycobacterium tuberculosis (Mtb) infection that enables the survival of this pathogen within phagocytic cells and modulates the immune response facilitating the spread of the tuberculosis d…
View article: Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways
Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways Open
High-hydrostatic pressure is an alternative perturbation method that can be used to destabilize globular proteins.Generally perfectly reversible, pressure exerts local effects on regions or domains of a protein containing internal voids, c…
View article: Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways
Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways Open
High-hydrostatic pressure is an alternative perturbation method that can be used to destabilize globular proteins. Generally perfectly reversible, pressure exerts local effects on regions or domains of a protein containing internal voids, …
View article: The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals a new Arpp19/PP2A-B55 feedback loop
The study of the determinants controlling Arpp19 phosphatase-inhibitory activity reveals a new Arpp19/PP2A-B55 feedback loop Open
Arpp19 is a potent inhibitor of PP2A-B55 that regulates this phosphatase to ensure the stable phosphorylation of mitotic/meiotic substrates. At G2-M, Arpp19 is phosphorylated by Greatwall on S67. This phosphorylated Arpp19 form displays a …
View article: Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds
Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds Open
Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities wit…
View article: Protein kinase B controls <i>Mycobacterium tuberculosis</i> growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112
Protein kinase B controls <i>Mycobacterium tuberculosis</i> growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112 Open
Summary Mycobacterium tuberculosis ( Mtb) is able to persist in the body through months of multi‐drug therapy. Mycobacteria possess a wide range of regulatory proteins, including the protein kinase B (PknB) which controls peptidoglycan bio…
View article: Structural genomic applied on the rust fungus <i>Melampsora larici-populina</i> reveals two candidate effector proteins adopting cystine-knot and nuclear transport factor 2-like protein folds
Structural genomic applied on the rust fungus <i>Melampsora larici-populina</i> reveals two candidate effector proteins adopting cystine-knot and nuclear transport factor 2-like protein folds Open
Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities wit…
View article: Folding of the Ig-Like Domain of the Dengue Virus Envelope Protein Analyzed by High-Hydrostatic-Pressure NMR at a Residue-Level Resolution
Folding of the Ig-Like Domain of the Dengue Virus Envelope Protein Analyzed by High-Hydrostatic-Pressure NMR at a Residue-Level Resolution Open
Dengue fever is a mosquito-borne endemic disease in tropical and subtropical regions, causing a significant public health problem in Southeast Asia. Domain III (ED3) of the viral envelope protein contains the two dominant putative epitopes…
View article: Protein kinase B controls <i>Mycobacterium tuberculosis</i> growth via phosphorylation of the global transcriptional regulator Lsr2
Protein kinase B controls <i>Mycobacterium tuberculosis</i> growth via phosphorylation of the global transcriptional regulator Lsr2 Open
Mycobacterium tuberculosis is able to persist in the body through months of multi-drug therapy. Mycobacteria possess a wide range of regulatory proteins, including the essential protein kinase B (PknB), that control transitions between gro…
View article: Structural basis for chemically-induced homodimerization of a single domain antibody
Structural basis for chemically-induced homodimerization of a single domain antibody Open
Chemically-induced dimerization (CID) systems are essential tools to interrogate and control biological systems. AcVHH is a single domain antibody homo-dimerizing upon caffeine binding. AcVHH has a strong potential for clinical application…
View article: Interplay of protein disorder in retinoic acid receptor heterodimer and its corepressor regulates gene expression
Interplay of protein disorder in retinoic acid receptor heterodimer and its corepressor regulates gene expression Open
The retinoic acid receptors (RARs) form heterodimers with retinoid X receptors (RXRs) and control gene transcription in response to ligand binding and via allosteric activation of the C-termini helix (helix H12) of its ligand-binding domai…
View article: A Tumor-Imaging Method Targeting Cancer-Associated Fibroblasts
A Tumor-Imaging Method Targeting Cancer-Associated Fibroblasts Open
The tumor stroma, which accounts for a large part of the tumor mass, represents an attractive target for the delivery of diagnostic and therapeutic compounds. Here, the focus is notably on a subpopulation of stromal cells, known as cancer-…