Phillip E. Klebba
YOU?
Author Swipe
View article: Siderophore-mediated iron acquisition by <i>Klebsiella pneumoniae</i>
Siderophore-mediated iron acquisition by <i>Klebsiella pneumoniae</i> Open
Microbes synthesize and secrete siderophores, that bind and solubilize precipitated or otherwise unavailable iron in their microenvironments. Gram (−) bacterial TonB-dependent outer membrane receptors capture the resulting ferric sideropho…
View article: Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu
Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu Open
We studied the Escherichia coli outer membrane protein Fiu, a presumed transporter of monomeric ferric catecholates, by introducing Cys residues in its surface loops and modifying them with fluorescein maleimide (FM). Fiu-FM bound iron com…
View article: Fluorescent sensors of siderophores produced by bacterial pathogens
Fluorescent sensors of siderophores produced by bacterial pathogens Open
Siderophores are iron-chelating molecules that solubilize Fe3+ for microbial utilization and facilitate colonization or infection of eukaryotes by liberating host iron for bacterial uptake. By fluorescently labeling membrane receptors and …
View article: Decision letter: Structural intermediates observed only in intact Escherichia coli indicate a mechanism for TonB-dependent transport
Decision letter: Structural intermediates observed only in intact Escherichia coli indicate a mechanism for TonB-dependent transport Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Outer membrane TonB-dependent…
View article: Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics
Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics Open
Iron is an indispensable metabolic cofactor in both pro- and eukaryotes, which engenders a natural competition for the metal between bacterial pathogens and their human or animal hosts. Bacteria secrete siderophores that extract Fe3+ from …
View article: Universal fluorescent sensors of high-affinity iron transport, applied to ESKAPE pathogens
Universal fluorescent sensors of high-affinity iron transport, applied to ESKAPE pathogens Open
Sensitive assays of biochemical specificity, affinity, and capacity are valuable both for basic research and drug discovery. We created fluorescent sensors that monitor high-affinity binding reactions and used them to study iron acquisitio…
View article: Fluorescence High-Throughput Screening for Inhibitors of TonB Action
Fluorescence High-Throughput Screening for Inhibitors of TonB Action Open
Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (F…
View article: TonB-Dependent Heme/Hemoglobin Utilization by Caulobacter crescentus HutA
TonB-Dependent Heme/Hemoglobin Utilization by Caulobacter crescentus HutA Open
Siderophore nutrition tests with Caulobacter crescentus strain NA1000 revealed that it utilized a variety of ferric hydroxamate siderophores, including asperchromes, ferrichromes, ferrichrome A, malonichrome, and ferric aerobactin, as well…
View article: Confined Mobility of TonB and FepA in Escherichia coli Membranes
Confined Mobility of TonB and FepA in Escherichia coli Membranes Open
The important process of nutrient uptake in Escherichia coli, in many cases, involves transit of the nutrient through a class of beta-barrel proteins in the outer membrane known as TonB-dependent transporters (TBDTs) and requires interacti…
View article: TonB and FepA Data for PLoS publication
TonB and FepA Data for PLoS publication Open
This dataset includes the single molecule fluorescence trajectories of TonB and FepA in E. coli membranes under several conditions at frame rates ranging from 60 – 1000 Hz as supplemental information for Lill et al., “Confined …
View article: ROSET Model of TonB Action in Gram-Negative Bacterial Iron Acquisition
ROSET Model of TonB Action in Gram-Negative Bacterial Iron Acquisition Open
The ro tational s urveillance and e nergy t ransfer (ROSET) model of TonB action suggests a mechanism by which the electrochemical proton gradient across the Gram-negative bacterial inner membrane (IM) promotes the transport of iron throug…
View article: High-Throughput Screening Assay for Inhibitors of TonB-Dependent Iron Transport
High-Throughput Screening Assay for Inhibitors of TonB-Dependent Iron Transport Open
The TonB-dependent Gram-negative bacterial outer membrane protein FepA actively transports the siderophore ferric enterobactin (FeEnt) into the periplasm. We developed a high-throughput screening (HTS) assay that observes FeEnt uptake thro…