Pierre-Yves Colin
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View article: Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase Open
Finding new mechanistic solutions for biocatalytic challenges is key in the evolutionary adaptation of enzymes, as well as in devising new catalysts. The recent release of man-made substances into the environment provides a dynamic testing…
View article: Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase
Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase Open
The recent massive release of new, man-made substances into the environment requires bioremediation, but a very limited number of enzymes evolved in response are available. When environments have not encountered the potentially hazardous m…
View article: Engineering improved ethylene production: Leveraging systems biology and adaptive laboratory evolution
Engineering improved ethylene production: Leveraging systems biology and adaptive laboratory evolution Open
View article: Functional and computational identification of a rescue mutation near the active site of an mRNA methyltransferase
Functional and computational identification of a rescue mutation near the active site of an mRNA methyltransferase Open
RNA-based drugs are an emerging class of therapeutics combining the immense potential of DNA gene-therapy with the absence of genome integration-associated risks. While the synthesis of such molecules is feasible, large scale in vitro prod…
View article: Investigating host-microbiome interactions by droplet based microfluidics
Investigating host-microbiome interactions by droplet based microfluidics Open
View article: Additional file 2 of Investigating host-microbiome interactions by droplet based microfluidics
Additional file 2 of Investigating host-microbiome interactions by droplet based microfluidics Open
Additional file 2. Table S3. Functional and taxonomical annotation of the metagenomic sequences.
View article: Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics Open
View article: Enzyme engineering in biomimetic compartments
Enzyme engineering in biomimetic compartments Open
View article: Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins
Bioinspired genotype–phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins Open
The idea of compartmentalization of genotype and phenotype in cells is key for enabling Darwinian evolution. This contribution describes bioinspired systems that use in vitro compartments—water-in-oil droplets and gel-shell beads—for the d…