Pino Bordignon YOU? Author Swipe

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Decoding Neuro-Immune Interactions: Spatial Multiomics Analysis of Mouse Brain via same-section RNA and Protein Co-detection 3572 Open

Pino Bordignon, Alice Comberlato, Daniel Azorín, Paula Juricic, Debia Wakhloo , et al. · 2025

Description The rise of spatial biology has transformed our ability to investigate tissue structures and cellular interactions at a single-cell resolution. Through techniques such as multiplexed immunofluorescence (mIF) or RNA in situ hybr…

1265 Unraveling tumor microenvironment through multiomics spatial mapping in fresh frozen samples Open

Cansaran Saygili Demir, Marion Bonnet, Alice Comberlato, Pino Bordignon, Debia Wakhloo , et al. · 2025

61 Fully automated multiomics assay for co-detection of protein-protein interactions, RNA and protein markers on the same tissue section Open

Alice Comberlato, Pino Bordignon, Arec Manoukian, Ge-Ah Kim, Sonali A. Deshpande , et al. · 2025

Screening for Tumor Microtube-Targeting Drugs Identifies PKC Modulators as Multipotent Inhibitors of Glioblastoma Progression Open

Daniel Domínguez Azorín, Dirk C. Hoffmann, Nils Hebach, Erik Jung, David Hausmann , et al. · 2025

Glioblastomas are incurable primary brain tumors that depend on neural-like cellular processes, tumor microtubes (TMs), to invade the brain. TMs also interconnect single tumor cells to a communicating multicellular network that resists cur…

81 Multiomic mapping of the brain: same-section, fully-automated spatial RNA and protein detection on mouse frozen tissues Open

Alice Comberlato, Daniel Domínguez Azorín, Arec Manoukian, Pino Bordignon, Paula Juricic , et al. · 2024

85 Novel fully automated multiomics assay for profiling immune cell landscape and activation states Open

Anushka Dikshit, Cansaran Saygili Demir, Rose Delvillar, Emerald Dikshit, Selma Demır , et al. · 2024

71 Integration of RNA in situ hybridization and sequential immunofluorescence for same-slide fully automated multi-omics analysis of the tumor microenvironment Open

Alice Comberlato, Arec Manoukian, Paula Juricic, Pino Bordignon, Alexandre Kehren , et al. · 2023

Background Spatial biology has transformed our understanding of the tumor microenvironment (TME) by enabling the study of tissue composition and intercellular interactions at a single-cell level while preserving spatial context.^1–3

Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics Open

François Rivest, Deniz Eroglu, Benjamin Pelz, Joanna Kowal, Alexandre Kehren , et al. · 2023

Tissues are complex environments where different cell types are in constant interaction with each other and with non-cellular components. Preserving the spatial context during proteomics analyses of tissue samples has become an important o…

Fully Automated Sequential Immunofluorescence (seqIF) for Hyperplex Spatial Proteomics Open

François Rivest, Deniz Eroglu, Benjamin Pelz, Joanna Kowal, Alexander Kehren , et al. · 2023

High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq Open

Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima, Archibald Enninful , et al. · 2023

38 Fully automated spatial multi-omics analysis to map the tumor microenvironment with single-cell resolution Open

Pino Bordignon, Alice Comberlato, Saška Brajkovic, Diego Dupouy · 2022

Background The tumor and its microenvironment are distinguished by highly heterogeneous cell types in dynamic evolution¹. In the past decades, the adoption of single-cell RNA sequencing technologies improved our understanding of…

37 Signal amplification strategies for in situ detection of low-expressed markers in multiplex immunofluorescence Open

Pino Bordignon, Paula Juri ccaron i cacute, Alexandre Kehren, Victor de Gautard, Saška Brajkovic , et al. · 2022

Background The characterization of the tumor microenvironment is essential for a deep understanding of tumor biology and the identification of personalized targeted therapies. By means of new spatial proteomic approaches, a detailed mappin…

Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping Open

Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima, Archibald Enninful , et al. · 2022

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measu…

Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping Open

Rong Fan, Yang Liu, Marcello DiStasio, Graham Su, Hiromitsu Asashima , et al. · 2022

Visualizing cellular heterogeneity by quantifying the dynamics of MAPK activity in live mammalian cells with synthetic fluorescent biosensors Open

Min Ma, Pino Bordignon, G. Paolo Dotto, Serge Pelet · 2020

NOTCH1 gene amplification promotes expansion of Cancer Associated Fibroblast populations in human skin Open

Atul Katarkar, Giulia Bottoni, Andrea Clocchiatti, Sandro Goruppi, Pino Bordignon , et al. · 2020

Visualizing cellular heterogeneity by quantifying the dynamics of MAPK activity in live mammalian cells with synthetic fluorescent biosensors Open

Min Ma, Pino Bordignon, G. Paolo Dotto, Serge Pelet · 2019

Mitogen-Activated Protein Kinases (MAPKs) control a wide array of cellular functions by transducing extracellular information into defined biological responses. In order to understand how these pathways are regulated, dynamic single cell m…

CSL controls telomere maintenance and genome stability in human dermal fibroblasts Open

Giulia Bottoni, Atul Katarkar, Beatrice Tassone, Soumitra Ghosh, Andrea Clocchiatti , et al. · 2019

Genomic instability is a hallmark of cancer. Whether it also occurs in Cancer Associated Fibroblasts (CAFs) remains to be carefully investigated. Loss of CSL/RBP-Jκ, the effector of canonical NOTCH signaling with intrinsic transcription re…

Dualism of FGF and TGF-β Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant Open

Pino Bordignon, Giulia Bottoni, Xiaoying Xu, Alma S. Popescu, Zinnia Truan , et al. · 2019

Androgen receptor functions as transcriptional repressor of cancer-associated fibroblast activation Open

Andrea Clocchiatti, Soumitra Ghosh, Maria-Giuseppina Procopio, Luigi Mazzeo, Pino Bordignon , et al. · 2018

The aging-associated increase of cancer risk is linked with stromal fibroblast senescence and concomitant cancer-associated fibroblast (CAF) activation. Surprisingly little is known about the role of androgen receptor (AR) signaling in thi…

Convergent roles of ATF3 and CSL in chromatin control of cancer-associated fibroblast activation Open

Dong‐Eun Kim, Maria-Giuseppina Procopio, Soumitra Ghosh, Seung‐Hee Jo, Sandro Goruppi , et al. · 2017

Cancer-associated fibroblasts (CAFs) are important for tumor initiation and promotion. CSL, a transcriptional repressor and Notch mediator, suppresses CAF activation. Like CSL, ATF3, a stress-responsive transcriptional repressor, is down-m…

Erratum: Corrigendum: Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation Open

Maria-Giuseppina Procopio, Csaba László, Dania Al Labban, Dong‐Eun Kim, Pino Bordignon , et al. · 2015

Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation Open

Maria-Giuseppina Procopio, Csaba László, Dania Al Labban, Dong‐Eun Kim, Pino Bordignon , et al. · 2015