Polina Sysa‐Shah
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View article: Cell Type-Specific Effects of miR-21 Loss Attenuate Tumor Progression in MYC-Driven Prostate Cancer
Cell Type-Specific Effects of miR-21 Loss Attenuate Tumor Progression in MYC-Driven Prostate Cancer Open
Aberrant microRNA expression is common in cancer, yet cell-type-specific microRNA activity in the tumor microenvironment (TME) remains poorly understood. Here, we show that germline deletion of miR-21 significantly attenuated the progressi…
View article: A murine model of <i>Trypanosoma brucei-</i> induced myocarditis and cardiac dysfunction
A murine model of <i>Trypanosoma brucei-</i> induced myocarditis and cardiac dysfunction Open
Trypanosoma brucei is a protozoan parasite that causes human and animal African trypanosomiases (HAT and AAT). Cardiac symptoms are commonly reported in HAT patients, and intracardiac parasites with accompanying myocarditis have been obser…
View article: <scp>ErbB2</scp> ‐ <scp>NOTCH1</scp> axis controls autophagy in cardiac cells
<span>ErbB2</span> ‐ <span>NOTCH1</span> axis controls autophagy in cardiac cells Open
Although the epidermal growth factor receptor 2 (ErbB2) and Notch1 signaling pathways have both significant roles in regulating cardiac biology, their interplay in the heart remains poorly investigated. Here, we present evidence of a cross…
View article: A murine model of<i>Trypanosoma brucei-</i>induced myocarditis and cardiac dysfunction
A murine model of<i>Trypanosoma brucei-</i>induced myocarditis and cardiac dysfunction Open
Trypanosoma brucei is a protozoan parasite that causes human and animal African trypanosomiases (HAT and AAT). Cardiac symptoms are commonly reported in HAT patients, and intracardiac parasites with accompanying myocarditis have been obser…
View article: Convergent alterations in the tumor microenvironment of MYC-driven human and murine prostate cancer
Convergent alterations in the tumor microenvironment of MYC-driven human and murine prostate cancer Open
The tissue microenvironment in prostate cancer is profoundly altered. While such alterations have been implicated in driving prostate cancer initiation and progression to aggressive disease, how prostate cancer cells and their precursors m…
View article: Animal Models of Prenatal Stress
Animal Models of Prenatal Stress Open
Maternal stress during pregnancy has been linked to chronic disease and cancer in offspring. We used prenatal stress animal models to study maternal stress during pregnancy at Johns Hopkins University from December 2017 to February 2019. T…
View article: Osteopontin/Secreted Phosphoprotein-1 Behaves as a Molecular Brake Regulating the Brain Translocator Protein-Dependent Neuroinflammatory Response to Chronic Viral Infection
Osteopontin/Secreted Phosphoprotein-1 Behaves as a Molecular Brake Regulating the Brain Translocator Protein-Dependent Neuroinflammatory Response to Chronic Viral Infection Open
Background Osteopontin (OPN) as a secreted signaling protein, is dramatically induced in response to cellular injury and neurodegeneration. Microglial inflammatory responses in the brain are tightly associated with the neuropathologic hall…
View article: Prenatal stress enhances NNK-induced lung tumors in A/J mice
Prenatal stress enhances NNK-induced lung tumors in A/J mice Open
Children born to women who experience stress during pregnancy have an increased risk of cancer in later life, but no previous animal studies have tested such a link. We questioned whether prenatal stress (PS) in A/J mice affected the devel…
View article: Imaging CAR T cell therapy with PSMA-targeted positron emission tomography
Imaging CAR T cell therapy with PSMA-targeted positron emission tomography Open
PET targeting a mutated PSMA enabled serial imaging of CAR T cell activity in a murine model of ALL.
View article: Peptide-based PET quantifies target engagement of PD-L1 therapeutics
Peptide-based PET quantifies target engagement of PD-L1 therapeutics Open
Immune checkpoint therapies have shown tremendous promise in cancer therapy. However, tools to assess their target engagement, and hence the ability to predict their efficacy, have been lacking. Here, we show that target engagement and tum…
View article: In Vivo Imaging With Confirmation by Histopathology for Increased Rigor and Reproducibility in Translational Research: A Review of Examples, Options, and Resources
In Vivo Imaging With Confirmation by Histopathology for Increased Rigor and Reproducibility in Translational Research: A Review of Examples, Options, and Resources Open
Preclinical noninvasive imaging can be an indispensable tool for studying animal models of disease. In vivo imaging to assess anatomical, functional, and molecular features requires verification by a comparison to the macroscopic and micro…
View article: Chemogenetics revealed: DREADD occupancy and activation via converted clozapine
Chemogenetics revealed: DREADD occupancy and activation via converted clozapine Open
DREADD not the designer compound Designer receptors exclusively activated by designer drugs (DREADDs) constitute a powerful chemogenetic strategy that can modulate nerve cell activity in freely moving animal preparations. Gomez et al. used…
View article: Regulatory Forum Opinion Piece*: Imaging Applications in Toxicologic Pathology—Recommendations for Use in Regulated Nonclinical Toxicity Studies
Regulatory Forum Opinion Piece*: Imaging Applications in Toxicologic Pathology—Recommendations for Use in Regulated Nonclinical Toxicity Studies Open
Available imaging systems for use in preclinical toxicology studies increasingly show utility as important tools in the toxicologic pathologist’s armamentarium, permit longitudinal evaluation of functional and morphological changes in tiss…
View article: [64Cu]XYIMSR-06: A dual-motif CAIX ligand for PET imaging of clear cell renal cell carcinoma
[64Cu]XYIMSR-06: A dual-motif CAIX ligand for PET imaging of clear cell renal cell carcinoma Open
Carbonic anhydrase IX (CAIX) is a cell surface enzyme that is over-expressed in approximately 95% of cases of clear cell renal cell carcinoma (ccRCC), the most common renal cancer. We synthesized and performed in vitro and in vivo evaluati…
View article: A humanized antibody for imaging immune checkpoint ligand PD-L1 expression in tumors
A humanized antibody for imaging immune checkpoint ligand PD-L1 expression in tumors Open
Antibodies targeting the PD-1/PD-L1 immune checkpoint lead to tumor regression and improved survival in several cancers. PD-L1 expression in tumors may be predictive of response to checkpoint blockade therapy. Because tissue samples might …
View article: Echocardiographic Characterization of a Murine Model of Hypertrophic Obstructive Cardiomyopathy Induced by Cardiac-specific Overexpression of Epidermal Growth Factor Receptor 2.
Echocardiographic Characterization of a Murine Model of Hypertrophic Obstructive Cardiomyopathy Induced by Cardiac-specific Overexpression of Epidermal Growth Factor Receptor 2. Open
Although rare, hypertrophic cardiomyopathy (HCM) with midventricular obstruction is often associated with severe symptoms and complications. None of the existing HCM animal models display this particular phenotype. Our group developed a mo…
View article: Bidirectional cross-regulation between ErbB2 and β-adrenergic signalling pathways
Bidirectional cross-regulation between ErbB2 and β-adrenergic signalling pathways Open
Our studies show that myocardial ErbB2 and βAR signalling are linked in a feedback loop with βAR activation leading to increased ErbB2 expression and activity, and increased ErbB2 activity regulating β2AR expression. Most importantly, ErbB…
View article: Imaging of carbonic anhydrase IX with an 111In-labeled dual-motif inhibitor
Imaging of carbonic anhydrase IX with an 111In-labeled dual-motif inhibitor Open
We developed a new scaffold for radionuclide-based imaging and therapy of clear cell renal cell carcinoma (ccRCC) targeting carbonic anhydrase IX (CAIX). Compound XYIMSR-01, a DOTA-conjugated, bivalent, low-molecular-weight ligand, has two…
View article: ErbB2 overexpression upregulates antioxidant enzymes, reduces basal levels of reactive oxygen species, and protects against doxorubicin cardiotoxicity
ErbB2 overexpression upregulates antioxidant enzymes, reduces basal levels of reactive oxygen species, and protects against doxorubicin cardiotoxicity Open
Levels of the HER2/ErbB2 protein in the heart are upregulated in some women during breast cancer therapy, and these women are at high risk for developing heart dysfunction after sequential treatment with anti-ErbB2/trastuzumab or doxorubic…
View article: Electrocardiographic Characterization of Cardiac Hypertrophy in Mice that Overexpress the ErbB2 Receptor Tyrosine Kinase.
Electrocardiographic Characterization of Cardiac Hypertrophy in Mice that Overexpress the ErbB2 Receptor Tyrosine Kinase. Open
Electrocardiography is an important method for evaluation and risk stratification of patients with cardiac hypertrophy. We hypothesized that the recently developed transgenic mouse model of cardiac hypertrophy (ErbB2(tg)) will display dist…