Explanipedia

Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance Open

Jun Yin, Hsueh-Ling Chen, Anna Grigsby-Brown, Yi He, Myriam L. Cotten , et al. · 2025

Glia-derived secretory factors are essential for brain development, physiology, and homeostasis, with their dysfunction linked to a variety of neurological disorders. Through genetic and biochemical approaches, we identified odorant bindin…

Molecular basis for Gβγ-SNARE-mediated inhibition of synaptic vesicle fusion Open

Anna R. Eitel, Benjamin K. Mueller, Ali İ. Kaya, Marian F. Young, Jackson B. Cassada , et al. · 2025

The postsynaptic density in excitatory synapses is composed of clustered, heterogeneous nanoblocks Open

Rong Sun, James P. Allen, Zhuqing Mao, Liana Wilson, Mariam Haider , et al. · 2025

The nanoscale organization of proteins within synapses is critical for maintaining and regulating synaptic transmission and plasticity. Here, we used cryo-electron tomography (cryo-ET) to directly visualize the three-dimensional architectu…

Ultrafast Isolation of Synaptic Terminals From Rat Brain for Cryo-Electron Tomography Analysis Open

Rong Sun, Qiangjun Zhou · 2025

Understanding the nanoscale organization and molecular rearrangement of synaptic components is critical for elucidating the mechanisms of synaptic transmission and plasticity. Traditional synaptosome isolation protocols involve multiple ce…

Glia-derived secretory fatty acid binding protein Obp44a regulates lipid storage and efflux in the developing<i>Drosophila</i>brain Open

Jun Yin, Hsueh-Ling Chen, Anna Grigsby-Brown, Yi He, Myriam L. Cotten , et al. · 2024

Glia derived secretory factors play diverse roles in supporting the development, physiology, and stress responses of the central nervous system (CNS). Through transcriptomics and imaging analyses, we have identified Obp44a as one of the mo…

Clostridioides difficile ferrosome organelles combat nutritional immunity Open

Hualiang Pi, Rong Sun, James R. McBride, Angela Kruse, Katherine N. Gibson‐Corley , et al. · 2023

A Scalable Spatial–Temporal Correlated Non-Stationary Channel Fading Generation Method Open

Sheng Fang, Tongbao Mao, Boyu Hua, Yuan Ding, Maozhong Song , et al. · 2023

To address the challenges of complex implementation structures and high hardware resource consumption in multiple-input multiple-output (MIMO) channel emulators, this paper proposes a hardware generation method for spatial–temporal correla…

Cryo-electron tomography reveals postsynaptic nanoblocks in excitatory synapses Open

Rong Sun, James P. Allen, Liana Wilson, Mariam Haider, Barış Alten , et al. · 2023

The nanoscale organization of proteins within synapses is critical for maintaining and regulating synaptic transmission and plasticity. Here, we use cryogenic electron tomography to directly visualize the three-dimensional architecture and…

Organizing the synaptic junctions Open

Qiangjun Zhou · 2023

Analysis of tripartite <span>Synaptotagmin‐1‐SNARE</span>‐complexin‐1 complexes in solution Open

Klaudia Jaczynska, Luis Esquivies, Richard A. Pfuetzner, Barış Alten, Kyle D. Brewer , et al. · 2022

Characterizing interactions of Synaptotagmin‐1 with the SNARE complex is crucial to understand the mechanism of neurotransmitter release. X‐ray crystallography revealed how the Synaptotagmin‐1 C 2 B domain binds to the SNARE complex throug…

Screening of Hydrocarbon-Stapled Peptides for Inhibition of Calcium-Triggered Exocytosis Open

Ying Lai, Michael J. Tuvim, Jeremy Leitz, John Jacob Peters, Richard A. Pfuetzner , et al. · 2022

The so-called primary interface between the SNARE complex and synaptotagmin-1 (Syt1) is essential for Ca 2+ -triggered neurotransmitter release in neuronal synapses. The interacting residues of the primary interface are conserved across di…

Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides Open

Ying Lai, Giorgio Fois, José R. Flores, Michael J. Tuvim, Qiangjun Zhou , et al. · 2022

Membrane fusion triggered by Ca 2+ is orchestrated by a conserved set of proteins to mediate synaptic neurotransmitter release, mucin secretion and other regulated exocytic processes 1–4 . For neurotransmitter release, the Ca 2+ sensitivit…

Screening of hydrocarbon-stapled peptides for inhibition of calcium-triggered exocytosis Open

Ying Lai, Michael J. Tuvim, Jeremy Leitz, John Jacob Peters, Richard A. Pfuetzner , et al. · 2022

The so-called primary interface between the SNARE complex and synaptotagmin-1 (Syt1) is essential for Ca 2+ -triggered neurotransmitter release in neuronal synapses. The interacting residues of the primary interface are conserved across di…

Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies Open

Barış Alten, Qiangjun Zhou, Ok‐Ho Shin, Luis Esquivies, Pei-Yi Lin , et al. · 2020

Structures of neurexophilin–neurexin complexes reveal a regulatory mechanism of alternative splicing Open

Steven C. Wilson, K. Ian White, Qiangjun Zhou, Richard A. Pfuetzner, Ucheor B. Choi , et al. · 2019

The pre-synaptic fusion machinery Open

Axel T. Brünger, Ucheor B. Choi, Ying Lai, Jeremy Leitz, K. Ian White , et al. · 2019

Resolving indexing ambiguities in X-ray free-electron laser diffraction patterns Open

Monarin Uervirojnangkoorn, Artem Y. Lyubimov, Qiangjun Zhou, William I. Weis, Axel T. Brünger · 2019

Processing X-ray free-electron laser (XFEL) diffraction images poses challenges, as an XFEL pulse is powerful enough to destroy or damage the diffracting volume and thereby yields only one diffraction image per volume. Moreover, the crysta…

NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin Open

Ucheor B. Choi, Minglei Zhao, K. Ian White, Richard A. Pfuetzner, Luis Esquivies , et al. · 2018

SNARE complex disassembly by the ATPase NSF is essential for neurotransmitter release and other membrane trafficking processes. We developed a single-molecule FRET assay to monitor repeated rounds of NSF-mediated disassembly and reassembly…

Author response: NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin Open

Ucheor B. Choi, Minglei Zhao, K. Ian White, Richard A. Pfuetzner, Luis Esquivies , et al. · 2018

Molecular Mechanisms of Fast Neurotransmitter Release Open

Axel T. Brünger, Ucheor B. Choi, Ying Lai, Jeremy Leitz, Qiangjun Zhou · 2018

This review summarizes current knowledge of synaptic proteins that are central to synaptic vesicle fusion in presynaptic active zones, including SNAREs (soluble N-ethylmaleimide sensitive factor attachment protein receptors), synaptotagmin…

Ca2+-Triggered Synaptic Vesicle Fusion Initiated by Release of Inhibition Open

Axel T. Brünger, Jeremy Leitz, Qiangjun Zhou, Ucheor B. Choi, Ying Lai · 2018

Molecular Mechanisms Controlling Neurotransmitter Release by the Primed SNARE-complexin-Synaptotagmin Complex Open

Qiangjun Zhou, Peng Zhou, Thomas C. Südhof, Axel T. Brünger · 2018

The primed SNARE–complexin–synaptotagmin complex for neuronal exocytosis Open

Qiangjun Zhou, Peng Zhou, Austin L. Wang, Dick Wu, Minglei Zhao , et al. · 2017

Towards Understanding the Molecular Mechanism of Synchronous Neurotransmitter Release Open

Qiangjun Zhou, Thomas C. Südhof, Axel T. Brünger · 2017

Influence of Soil Surface Sodium Ion and Soil pH on Dispersion of Cohesive Soil Open

Qiangjun Zhou, Hui-bin Yu · 2016

The grey relational analysis method based on grey system theory is used, to make theoretical analysis of the chemical factors influencing the cohesive soil dispersion, and the pinhole test and pieces test are adopted to make further experi…

XFEL Diffraction data from Synaptotagmin-1 / SNARE complex, source of 5KJ7 structure Open

Artem Y. Lyubimov, Monarin Uervirojnangkoorn, Minglei Zhao, Qiangjun Zhou, Axel T. Brünger · 2016

The XFEL data were collected at the X-ray Pump Probe (XPP) endstation of the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, using a goniometer-based fixed target sample delivery station and an automatic sam…

Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex Open

Artem Y. Lyubimov, Monarin Uervirojnangkoorn, Oliver B. Zeldin, Qiangjun Zhou, Minglei Zhao , et al. · 2016

X-ray free electron lasers (XFELs) reduce the effects of radiation damage on macromolecular diffraction data and thereby extend the limiting resolution. Previously, we adapted classical post-refinement techniques to XFEL diffraction data t…

Author response: Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex Open

Artem Y. Lyubimov, Monarin Uervirojnangkoorn, Oliver B. Zeldin, Qiangjun Zhou, Minglei Zhao , et al. · 2016

Article Figures and data Abstract Introduction Results and discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract X-ray free electron lasers (XFELs) red…

Molecular Mechanism of the Synaptotagmin-SNARE Complex that is Essential for Synchronous Synaptic Neurotransmitter Release Open

Qiangjun Zhou, Axel T. Brünger · 2016

Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis Open

Qiangjun Zhou, Ying Lai, Taulant Bacaj, Minglei Zhao, Artem Y. Lyubimov , et al. · 2015

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