Explanipedia

Development of a Blocking ELISA for Detection of Serum Neutralizing Antibodies Against Duck Adenovirus Type 3 Open

Mei Jie Tang, Xiaona Shi, Qi Zhu, Minfan Huang, Bangfeng Xu , et al. · 2025

In 2014, Duck Adenovirus type 3 (DAdV-3) emerged in Muscovy ducks and has since spread rapidly across China, causing significant economic losses to the duck industry. Given this situation, the development of reliable diagnostic tools is cr…

Pathogenicity Evaluation and Virulence Gene Identification of an Attenuated Duck Enteritis Virus Open

Xiaona Shi, Haibin Zhuang, Dun Shuo, Luzhao Li, Shenghui Pan , et al. · 2025

Duck enteritis virus (DEV), an epornitic pathogen, causes substantial economic losses in the commercial duck industry and poses persistent risks to wild and migratory waterfowl populations. However, due to the large genomic capacity of the…

Genetic and Pathogenic Characteristics of Variant Avian Reovirus Strains Isolated from Diseased Chickens in China Open

Shiqi Niu, Zihua Wu, Shenghui Pan, Tianxin Ma, Yunxiang Zhang , et al. · 2025

Avian viral arthritis (AVA), caused by avian reovirus (ARV), is a viral disease in chickens that has led to significant economic losses in the poultry industry. Recent studies have shown that traditional ARV vaccines based on the S1133 str…

Transmission routes of cluster 3 Tembusu virus in ducks and chickens Open

Luzhao Li, Dawei Yan, Dun Shuo, Xiaona Shi, Minghao Yan , et al. · 2025

Tembusu virus (TMUV) is an emerging mosquito-borne flavivirus, primarily transmitted by Culex spp. mosquitoes. The 2010 outbreak of TMUV in ducks revealed that the virus had acquired direct contact and aerosol transmission routes, enabling…

The Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Strain Open

Haipeng Lu, Mei Tang, Zhifei Zhang, Mi Wu, Chunxiu Yuan , et al. · 2025

A duck adenovirus type 3 strain, SD2019, was isolated from sick Muscovy ducks in our laboratory in 2019. To study the biological properties of the virus, an infectious clone of the SD2019 strain was successfully established. The plasmid co…

Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Open

Haipeng Lu, Mei Tang, Zhifei Zhang, Mi Wu, Chunxiu Yuan , et al. · 2025

A duck adenovirus type 3 strain SD2019 was isolated from sick Muscovy ducks in our laboratory in 2019. To study the biological properties of the virus, an infectious clone of the SD2019 strain was successfully established. The plasmid cont…

Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome Open

Zhifei Zhang, Xin Su, Dun Shuo, Dawei Yan, Xue Pan , et al. · 2025

Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial…

Isolation and Characterization of H1 Subtype Swine Influenza Viruses Recently Circulating in China Open

Minghao Yan, Tianxin Ma, Xiaona Shi, Qin Chen, Luzhao Li , et al. · 2025

Pigs serve as a mixing vessel for influenza viruses and can independently promote the emergence of pandemic strains in humans. During our surveillance of pig populations from 2021 to 2023 in China, 11 H1 subtype swine influenza viruses (SI…

The Generation of a H9N2 Avian Influenza Virus with HA and C3d-P29 Protein Fusions and Vaccine Development Applications Open

Xue Pan, Fan Zhou, Xiaona Shi, Qinfang Liu, Dawei Yan , et al. · 2025

Background: Maternal-derived antibody (MDA) interferes with immune responses, leading to the failure of H9N2 avian influenza vaccinations in poultry. So far, none of the commercially available H9N2 avian influenza vaccines used in poultry …

hnRNPH1 Inhibits Influenza Virus Replication by Binding Viral RNA Open

Ruixue Xue, Danqi Bao, Tianxin Ma, Shiqi Niu, Zihua Wu , et al. · 2024

During the life cycle of the influenza virus, viral RNPs (vRNPs) are transported to the nucleus for replication. Given that a large number of progeny viral RNA occupies the nucleus, whether there is any host protein located in the nucleus …

Generation of a H9N2 Avian Influenza Virus Whose Ha Proteins Fused C3d-P29 and Its Application in Vaccine Development Open

Xue Pan, Fan Zhou, Xiaona Shi, Qinfang Liu, Dawei Yan , et al. · 2024

Maternal-derived antibodies (MDAs) interfere with immune responses, leading to the failure of H9N2 avian influenza vaccination in poultry. So far, none of commercial H9N2 avian influenza vaccines used in poultry can overcome MDAs interfere…

Genome-wide CRISPR screen identifies GNE as a key host factor that promotes influenza A virus adsorption and endocytosis Open

Tianxin Ma, Shiqi Niu, Zihua Wu, Shenghui Pan, Chenyang Wang , et al. · 2023

Replication of influenza A virus (IAV) is highly reliant on host cell function, and to identify the key host factor required in the influenza A virus life cycle, a genome-wide CRISPR/Cas9 knockout (KO) screen was conducted in A549 cells in…

Generation and characterization of chimeric Tembusu viruses containing pre-membrane and envelope genes of Japanese encephalitis virus Open

Bangfeng Xu, Xingpo Liu, Dawei Yan, Qiaoyang Teng, Chunxiu Yuan , et al. · 2023

Since its outbreak in 2010, Tembusu virus (TMUV) has spread widely throughout China and Southeast Asia, causing significant economic losses to the poultry industry. In 2018, an attenuated vaccine called FX2010-180P (180P) was licensed for …

Immunoadjuvant efficacy of CpG plasmids for H9N2 avian influenza inactivated vaccine in chickens with maternal antibodies Open

Xue Pan, Qinfang Liu, M.C.M. de Jong, Maria Forlenza, Shiqi Niu , et al. · 2023

Maternal-derived antibodies (MDAs) are one of reasons why vaccination with the H9N2 inactivated whole virus (IWV) vaccine failed in poultry. Unmethylated CpG motif-containing oligodeoxynucleotides (CpG ODN) shows great potential to overcom…

Efficacy of a recombinant turkey herpesvirus (H9) vaccine against H9N2 avian influenza virus in chickens with maternal-derived antibodies Open

Xue Pan, Qinfang Liu, Shiqi Niu, Dongming Huang, Dawei Yan , et al. · 2023

Although vaccines have been widely used for many years, they have failed to control H9N2 avian influenza virus (AIV) in the field in China. The high level of maternal-derived antibodies (MDAs) against H9N2 virus contributes to the H9N2 inf…

Biological Characteristics of Infectious Laryngotracheitis Viruses Isolated in China Open

Mi Wu, Zhifei Zhang, Xin Su, Haipeng Lu, Xuesong Li , et al. · 2022

Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in chickens and results in huge economic losses in the poultry industry worldwide. To correlate the genomic difference with the replication and pathogenicity, phen…

Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field Open

Xue Pan, Xin Su, Pingyun Ding, Jinhua Zhao, Hongrui Cui , et al. · 2022

An Emerging Duck Egg-Reducing Syndrome Caused by a Novel Picornavirus Containing Seven Putative 2A Peptides Open

Xin Su, Dun Shuo, Yanqiu Luo, Xue Pan, Dawei Yan , et al. · 2022

Since 2016, frequent outbreaks of egg-reducing syndromes caused by an unknown virus in duck farms have resulted in huge economic losses in China. The causative virus was isolated and identified as a novel species in Avihepatovirus of the p…

Hydrophobic Residues at the Intracellular Domain of the M2 Protein Play an Important Role in Budding and Membrane Integrity of Influenza Virus Open

Danqi Bao, Chenyang Lu, Tianxin Ma, Guanlong Xu, Yaqing Mao , et al. · 2022

M2 plays a crucial role in the influenza virus life cycle. However, the function of the C-terminal intracellular domain of M2 protein remains largely unclear.

A Single Mutation at Position 120 in the Envelope Protein Attenuates Tembusu Virus in Ducks Open

Dawei Yan, Binbin Wang, Ying Shi, Xintao Ni, Xiaogang Wu , et al. · 2022

A live attenuated duck Tembusu virus (TMUV) vaccine FX2010-180P (180P) was successfully utilized to prevent TMUV infections in ducks in China. Compared with wild-type TMUV, 180P was highly attenuated and lost transmissibility in ducks. How…

The PB1 gene from H9N2 avian influenza virus showed high compatibility and increased mutation rate after reassorting with a human H1N1 influenza virus Open

Hongrui Cui, Guangsheng Che, M.C.M. de Jong, Xuesong Li, Qinfang Liu , et al. · 2022

Molecular and Antigenic Characterization of Avian H9N2 Viruses in Southern China Open

Wanwan Yan, Hongrui Cui, M.Y. Engelsma, Nancy Beerens, Monique M. van Oers , et al. · 2022

The H9N2 low pathogenicity avian influenza (LPAI) virus has become endemic in poultry globally. In several Asian countries, vaccination against H9N2 avian influenza virus (AIV) was approved to reduce economic losses in the poultry industry.

Additional file 1 of Different serotypes of Escherichia coli flagellin exert identical adjuvant effects Open

Shengmei Pang, Wenwen Wu, Qinfang Liu, Guoqiang Zhu, Qiangde Duan · 2022

Additional file 1.

Additional file 2 of The PB1 gene from H9N2 avian influenza virus showed high compatibility and increased mutation rate after reassorting with a human H1N1 influenza virus Open

Hongrui Cui, Guangsheng Che, M.C.M. de Jong, Xuesong Li, Qinfang Liu , et al. · 2022

Additional file 2: Table S1. Specific indexes (Barcodes) for reassortant viruses from different hosts. Table S2 Primer sequence for segment amplification of HA gene. Table S3 Total substitution frequency (Fi) at every location at HA ORF re…

Review for "Characterisation, whole‐genome sequencing and phylogenetic analysis of three H3N2 avian influenza viruses isolated from domestic ducks at live poultry markets of Iran, 2017: First report" Open

Qinfang Liu · 2021

RNA-Seq Analysis of Influenza A Virus-Induced Transcriptional Changes in Mice Lung and Its Possible Implications for the Virus Pathogenicity in Mice Open

Tianxin Ma, Abdou Nagy, Guanlong Xu, Lingxiang Xin, Danqi Bao , et al. · 2021

The influenza A virus (IAV) is an important cause of respiratory disease worldwide. It is well known that alveolar epithelial cells are the target cells for the IAV, but there is relatively limited knowledge regarding the role of macrophag…

The PB1 Gene from H9N2 Avian Influenza Virus Showed High Compatibility and Increased Mutation Rate after Reassorting with a Human H1N1 Influenza Virus Open

Hongrui Cui, Guangsheng Che, M.C.M. de Jong, Xuesong Li, Qinfang Liu , et al. · 2021

BackgroundReassortment between human and avian influenza viruses (AIV) may result in novel viruses with new characteristics that may threaten human health when causing the next flu pandemic. A particular risk may be posed by avian influenz…

Vaccination with inactivated virus against low pathogenic avian influenza subtype H9N2 does not prevent virus transmission in chickens Open

Hongrui Cui, M.C.M. de Jong, Nancy Beerens, Monique M. van Oers, Qiaoyang Teng , et al. · 2021

Key Amino Acids of M1-41 and M2-27 Determine Growth and Pathogenicity of Chimeric H17 Bat Influenza Virus in Cells and in Mice Open

Jianmei Yang, Pei Zhang, Min Huang, Shuyuan Qiao, Qinfang Liu , et al. · 2021

The M1 and M2 proteins influence the morphology, replication, virulence, and transmissibility of influenza viruses. Although a few key residues in the M1 and M2 proteins have been identified, whether other residues of the M1 and M2 protein…

Caspase-Dependent Cleavage of DDX21 Suppresses Host Innate Immunity Open

Wei Wu, Yang Qu, Shengqing Yu, Sa Wang, Yuncong Yin , et al. · 2021

Innate immunity serves as the first barrier against virus infection. DEAD (Glu-Asp-Ala-Glu) box RNA helicases, originally considered to be involved in RNA processing and RNA unwinding, have been shown to play an important role in antiviral…

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