Explanipedia

Sustainable Production of Ginsenosides: Advances in Biosynthesis and Metabolic Engineering Open

Yang Xue, Ruixiang Zhang, Tie Li, Q. Deng, Weike Luo , et al. · 2025

Ginsenosides, the primary bioactive components of Panax ginseng, exhibit diverse pharmacological properties, ranging from anticancer to neuroprotective effects. However, traditional production by ginseng cultivation faces limitations due t…

PlantGPT: An Arabidopsis‐Based Intelligent Agent that Answers Questions about Plant Functional Genomics (Adv. Sci. 30/2025) Open

Ruixiang Zhang, Yu Wang, Weiyang Yang, Jun Wen, Weizhi Liu , et al. · 2025

Synthetic metabolic engineering of functional crops: Boosting nutrition and human health Open

Nan Chai, Jie Xu, Ruixiang Zhang, Guangzhou Li, Jun Wen , et al. · 2025

Programmable genome engineering and gene modifications for plant biodesign Open

Jialin Liu, Ruixiang Zhang, Nan Chai, Su Liu, Zhiye Zheng , et al. · 2025

Engineering Adenine Deaminase TadA for Precise and PAM‐Flexible Point Mutagenesis and Gradient‐Tuning Endogenous Protein Design Open

Kangli Sun, Si Cheng, Nan Chai, Jianing Mi, Ruixiang Zhang , et al. · 2025

Base editing enables precise nucleotide substitutions within a relatively broad editing window (5–6 nucleotides). However, considerable bystander editing significantly compromise its accuracy. Point mutagenesis, a powerful approach for gra…

Metabolic engineering of theanine biosynthesis in rice endosperm to develop biofortified theaRice for benefiting human health Open

Ke Chen, Kangli Sun, Chanjuan Ye, Yutong Liu, Y. J. Hu , et al. · 2025

Development of PAM-flexible multiplex genome editors for enhancing cold-tolerance in indica rice Open

Jiantao Tan, Kangli Sun, Qian Qian, Nan Chai, Xin Peng , et al. · 2025

The canonical CRISPR/Cas editors are constrained by the requirement for specific PAMs, which substantially limits their editable target range. Rice (particularly indica rice) is sensitive to low temperature, which impacts the yield and res…

SuperDecode: An integrated toolkit for analyzing mutations induced by genome editing Open

Fuquan Li, Xiyu Tan, Shengting Li, Shaotong Chen, Lin Liu , et al. · 2025

Genome editing using CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) or other systems has become a cornerstone of numerous biological and applied research fields. However, detecting the resu…

Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals Open

Haixin Gao, Xinyi Pei, Xinyu Song, Shiying Wang, Zaibin Yang , et al. · 2025

As an efficient gene editing tool, the CRISPR/Cas9 system has been widely employed to investigate and regulate the biosynthetic pathways of active ingredients in medicinal plants. CRISPR technology holds significant potential for enhancing…

PhieDBEs: a DBD‐containing, PAM‐flexible, high‐efficiency dual base editor toolbox with wide targeting scope for use in plants Open

Zhiye Zheng, Taoli Liu, Nan Chai, Dongchang Zeng, Ruixiang Zhang , et al. · 2024

Summary Dual base editors (DBEs) enable simultaneous A‐to‐G and C‐to‐T conversions, expanding mutation types. However, low editing efficiency and narrow targeting range limit the widespread use of DBEs in plants. The single‐strand DNA bind…

Exploring C-to-G and A-to-Y Base Editing in Rice by Using New Vector Tools Open

Dongchang Zeng, Zhiye Zheng, Yuxin Liu, Taoli Liu, Tie Li , et al. · 2022

CRISPR/Cas9-based cytosine base editors (CBEs) and adenine base editors (ABEs) can efficiently mediate C-to-T/G-to-A and A-to-G/T-to-C substitutions, respectively; however, achieving base transversions (C-to-G/C-to-A and A-to-T/A-to-C) is …

Efficient assembly of long DNA fragments and multiple genes with improved nickase‐based cloning and Cre/loxP recombination Open

Yanchang Zhao, Jingluan Han, Jiantao Tan, Yaqian Yang, Shuangchun Li , et al. · 2022

Summary Functional genomics, synthetic biology and metabolic engineering require efficient tools to deliver long DNA fragments or multiple gene constructs. Although numerous DNA assembly methods exist, most are complicated, time‐consuming …

Bioinformatic and functional analyses reveal the expansion base of the rice polyketide synthase III superfamily and the synergistic roles of OsPKS1 and OsPKS2 in male reproduction Open

Kun Ma, Zhongfang Yang, Bowen Yang, Yao‐Guang Liu, Qinlong Zhu , et al. · 2022

Type III polyketide synthase (PKS III) superfamily members are involved in plant secondary metabolism, which is important for plant development and resistance to biological and abiotic stress. However, the functions of most PKS III superfa…

BEtarget: A versatile web-based tool to design guide RNAs for base editing in plants Open

Xianrong Xie, Fuquan Li, Xiyu Tan, Dongchang Zeng, Weizhi Liu , et al. · 2022

Quantitative regulation of Waxy expression by CRISPR/Cas9‐based promoter and 5'UTR‐intron editing improves grain quality in rice Open

Dongchang Zeng, Taoli Liu, Xingliang Ma, Bin Wang, Zhiye Zheng , et al. · 2020

In cereal crops, grain starches are composed of different proportions of amylose and amylopectin, which determine the cooking and eating qualities. The amylose synthesis is controlled by the Waxy (Wx) gene encoding a granule bound NDP-gluc…

Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice Open

Rongxin Shen, Lan Wang, Xupeng Liu, Jiang Wu, Weiwei Jin , et al. · 2017

Targeted Genome Editing in Genes and cis-Regulatory Regions Improves Qualitative and Quantitative Traits in Crops Open

Xitao Li, Yongyao Xie, Qinlong Zhu, Yao‐Guang Liu · 2017

Once for All: A Novel Robust System for Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in Plants Open

Guitao Zhong, Qinlong Zhu, Yingxin Li, Yao‐Guang Liu, Hao Wang · 2017

Chimeric fluorescent fusion proteins have been employed as a powerful tool to reveal the subcellular localizations and dynamics of proteins in living cells. Co-expression of a fluorescent fusion protein with well-known organelle markers in…

Genome-Wide Association and Transcriptome Analyses Reveal Candidate Genes Underlying Yield-determining Traits in Brassica napus Open

Kun Lu, Peng Liu, Chao Zhang, Junhua Lu, Bo Yang , et al. · 2017

Yield is one of the most important yet complex crop traits. To improve our understanding of the genetic basis of yield establishment, and to identify candidate genes responsible for yield improvement in Brassica napus, we performed …

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications Open

Xingliang Ma, Qinlong Zhu, Yuanling Chen, Yao‐Guang Liu · 2016

Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco Open

Qinlong Zhu, Shunzhao Sui, Xinghua Lei, Zhongfang Yang, Kun Lu , et al. · 2015

Proanthocyanidins (PAs) play an important role in plant disease defense and have beneficial effects on human health. We isolated and characterized a novel R2R3 MYB-type PA-regulator SsMYB3 from a well-known ornamental plant, coleus (Soleno…

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Open

Xingliang Ma, Qunyu Zhang, Qinlong Zhu, Wei Liu, Yan Chen , et al. · 2015

Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products Open

Xingliang Ma, Letian Chen, Qinlong Zhu, Yuanling Chen, Yao‐Guang Liu · 2015

XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice Open

Chunlian Wang, Xiaoping Zhang, Yinglun Fan, Ying Gao, Qinlong Zhu , et al. · 2015

Qinlong Zhu YOU? Author Swipe