Qinxia Han
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View article: Systematic analysis of a mitochondrial disease‐causing <i>ND6</i> mutation in mitochondrial deficiency
Systematic analysis of a mitochondrial disease‐causing <i>ND6</i> mutation in mitochondrial deficiency Open
Background The m.14487T>C mutation is recognized as a diagnostic mutation of mitochondrial disease during the past 16 years, emerging evidence suggests that mutant loads of m.14487T>C and disease phenotype are not closely correlated. Metho…
View article: Expression of soluble native protein in Escherichia coli using a cold-shock SUMO tag-fused expression vector
Expression of soluble native protein in Escherichia coli using a cold-shock SUMO tag-fused expression vector Open
At present, approximately 30% of eukaryotic proteins can be expressed in a soluble form in Escherichia coli. In this study, a pCold-SUMOa plasmid was constructed in order to express heterologous proteins fused with SUMO by a cold-shock exp…