Explanipedia

Lambda‐PCR for precise DNA assembly and modification Open

Imen Tanniche, Amanda K. Fisher, Frank Gillam, Eva Collakova, Chenming Zhang , et al. · 2022

Lambda‐polymerase chain reaction (λ‐PCR) is a novel and open‐source method for DNA assembly and cloning projects. λ‐PCR uses overlap extension to ultimately assemble linear and circular DNA fragments, but it allows the single‐stranded DNA …

Sphingosine Kinase 2 Inhibitors: Rigid Aliphatic Tail Derivatives Deliver Potent and Selective Analogues Open

Srinath Pashikanti, Daniel Foster, Yugesh Kharel, Anne M. Brown, David R. Bevan , et al. · 2022

Sphingosine 1-phosphate (S1P) is a pleiotropic signaling molecule that interacts with five native G-protein coupled receptors (S1P1-5) to regulate cell growth, survival, and proliferation. S1P has been implicated in a variety of pathologie…

Biophysical insights into OR2T7: Investigation of a potential prognostic marker for glioblastoma Open

Amanda K. Sharp, David Newman, Gianna Libonate, Mary Borns-Stern, David R. Bevan , et al. · 2022

Simulations of cross-amyloid aggregation of amyloid-β and islet amyloid polypeptide fragments Open

Grant Kawecki, Kelsie M. King, Nicholas A. Cramer, David R. Bevan, Anne M. Brown · 2022

Insight into Cross-Amyloid Interactions and Morphologies: Molecular Dynamics Simulations of Model Peptide Fragments of Amyloid-β (Aβ<sub>16-22</sub>) and Islet Amyloid Polypeptide (IAPP<sub>20-29</sub>) Open

Nicholas A. Cramer, Grant Kawecki, Kelsie M. King, David R. Bevan, Anne M. Brown · 2021

Amyloid-beta (Aβ) and islet amyloid polypeptide (IAPP) are small peptides, classified as amyloids, that have the potential to self-assemble and form cytotoxic species, such as small soluble oligomers and large insoluble fibrils. The format…

Rosmarinic Acid Potently Detoxifies Amylin Amyloid and Ameliorates Diabetic Pathology in a Transgenic Rat Model of Type 2 Diabetes Open

Ling Wu, Paul Velander, Anne M. Brown, Yao Wang, Dongmin Liu , et al. · 2021

Protein aggregation is associated with a large number of human protein-misfolding diseases, yet FDA-approved drugs are currently not available. Amylin amyloid and plaque depositions in the pancreas are hallmark features of type 2 diabetes.…

Probing the substitution pattern of indole-based scaffold reveals potent and selective sphingosine kinase 2 inhibitors Open

Molly D. Congdon, Russell G. Fritzemeier, Yugesh Kharel, Anne M. Brown, Vlad Serbulea , et al. · 2020

Catechol-Containing Compounds are a Broad Class of Protein Aggregation Inhibitors: II. Rosmarinic Acid Potently Detoxifies Amylin Amyloid and Ameliorates Diabetic Pathology in HIP Rats Open

Ling Wu, Paul Velander, Anne M. Brown, Yao Wang, Dongmin Liu , et al. · 2020

Protein aggregation is associated with a large number of human protein misfolding diseases, yet FDA-approved drugs are currently not available. Amylin amyloid and plaque depositions in the pancreas are hallmark features of type 2 diabetes.…

Lipophilic tail modifications of 2-(hydroxymethyl)pyrrolidine scaffold reveal dual sphingosine kinase 1 and 2 inhibitors Open

Hao Li, Christopher D. Sibley, Yugesh Kharel, Tao Huang, Anne M. Brown , et al. · 2020

Insight into amyloid interactions and aggregation processes: Molecular dynamics simulations of amyloid fragments and full-length oligomers Open

Anne M. Brown, Nickolas A. Cramer, Grant Kawecki, David Barto, David R. Bevan · 2020

Insight into amyloid interactions and aggregation processes: Molecular dynamics simulations of amyloid fragments and full-length oligomers Open

Anne M. Brown, Nickolas A. Cramer, Grant Kawecki, David Barto, David R. Bevan · 2020

Insight into amyloid interactions and aggregation processes: Molecular dynamics simulations of amyloid fragments and full-length oligomers Open

Anne M. Brown, Nickolas A. Cramer, Grant Kawecki, David Barto, David R. Bevan · 2020

Discovery of a Small Side Cavity in Sphingosine Kinase 2 that Enhances Inhibitor Potency and Selectivity Open

Christopher D. Sibley, Emily A. Morris, Yugesh Kharel, Anne M. Brown, Tao Huang , et al. · 2020

The sphingosine-1-phosphate (S1P) signaling pathway is an attractive drug target due to its involvement in immune cell chemotaxis and vascular integrity. The formation of S1P is catalyzed by sphingosine kinase 1 or 2 (SphK1 or SphK2) from …

Molecular evolution of genes encoding allergen proteins in the peanuts genus Arachis: Structural and functional implications Open

Khidir W. Hilu, Sheena Anne Friend, Viruthika Vallanadu, Anne M. Brown, Louis Hollingsworth , et al. · 2019

Food allergies are severe immune responses to plant and animal products mediated by immunoglobulin E (IgE). Peanuts (Arachis hypogaea L.) are among the top 15 crops that feed the world. However, peanuts is among the "big eight food allerge…

Insight into Amyloid Interactions: Molecular Dynamics Simulations of Model Peptide Fragments Open

Nicholas A. Cramer, Grant Kawecki, David R. Bevan, Anne M. Brown · 2019

Molecular Dynamics Simulations of gp120 and gp41 of HIV Env Provide Insights into Strain Specificity and the Role of the Membrane Environment Open

Louis Hollingsworth, Justin A. Lemkul, Richard D. Gandour, David R. Bevan, Anne M. Brown · 2019

Curare: from laboratory to law court Open

David R. Bevan · 2018

Dr. David Bevan held the Wesley-Bourne Chair of Anesthesia at McGill University, Chair of Anesthesia at UBC, Anesthetist-in-Chief at the University Health Network/Mount Sinai Hospital and subsequently Chair of the Department of Anesthesia …

1978: Forty years later Open

Robert Bortolussi, Stuart MacLeod, David R. Bevan, Jonathan B. Angel · 2018

Think back; think wayyy back: before laptops, internet and smartphones. Bank machines didn’t exist, tele-phones were permanently plugged into the wall, airport security meant only checking that you paid for your ticket and medical journals…

HIV-1 Env gp41 Transmembrane Domain Dynamics Are Modulated by Lipid, Water, and Ion Interactions Open

Louis Hollingsworth, Justin A. Lemkul, David R. Bevan, Anne M. Brown · 2018

HIV-1 Env gp41 Transmembrane Domain Dynamics are Modulated by Lipid, Water, and Ion Interactions Open

Louis Hollingsworth, Justin A. Lemkul, David R. Bevan, Anne M. Brown · 2018

The gp41 transmembrane domain (TMD) of the envelope glycoprotein (Env) of the human immunodeficiency virus (HIV) modulates the conformation of the viral envelope spike, the only druggable target on the surface of the virion. Understanding …

Computational study of HIV gp120 as a target for polyanionic entry inhibitors: Exploiting the V3 loop region Open

Louis Hollingsworth, Anne M. Brown, Richard D. Gandour, David R. Bevan · 2018

Multiple approaches are being utilized to develop therapeutics to treat HIV infection. One approach is designed to inhibit entry of HIV into host cells, with a target being the viral envelope glycoprotein, gp120. Polyanionic compounds have…

Transforming Sphingosine Kinase 1 Inhibitors into Dual and Sphingosine Kinase 2 Selective Inhibitors: Design, Synthesis, and in Vivo Activity Open

Elizabeth S. Childress, Yugesh Kharel, Anne M. Brown, David R. Bevan, Kevin R. Lynch , et al. · 2017

Sphingosine 1-phosphate (S1P) is a pleiotropic signaling molecule that interacts with its five G-protein coupled receptors (S1P_1-5) to regulate cell growth and survival and has been implicated in a variety of diseases including …

Molecular Dynamics Simulations of Amyloid β -Peptide (1-42): Tetramer Formation and Membrane Interactions Open

Anne M. Brown, David R. Bevan · 2016

Development of a structured undergraduate research experience: Framework and implications Open

Anne M. Brown, Stephanie N. Lewis, David R. Bevan · 2016

Participating in undergraduate research can be a pivotal experience for students in life science disciplines. Development of critical thinking skills, in addition to conveying scientific ideas in oral and written formats, is essential to e…

Structure–Activity Relationship Studies and Molecular Modeling of Naphthalene-Based Sphingosine Kinase 2 Inhibitors Open

Molly D. Congdon, Yugesh Kharel, Anne M. Brown, Stephanie N. Lewis, David R. Bevan , et al. · 2016

The two isoforms of sphingosine kinase (SphK1 and SphK2) are the only enzymes that phosphorylate sphingosine to sphingosine-1-phosphate (S1P), which is a pleiotropic lipid mediator involved in a broad range of cellular processes including …

Molecular modeling of the elastomeric properties of repeating units and building blocks of resilin, a disordered elastic protein Open

Md Shahriar K. Khandaker, Daniel Dudek, Eric P. Beers, David Dillard, David R. Bevan · 2016

Phosphorylation of PPARγ Affects the Collective Motions of the PPARγ-RXRα-DNA Complex Open

Justin A. Lemkul, Stephanie N. Lewis, Josep Bassaganya‐Riera, David R. Bevan · 2015

Peroxisome-proliferator activated receptor-γ (PPARγ) is a nuclear hormone receptor that forms a heterodimeric complex with retinoid X receptor-α (RXRα) to regulate transcription of genes involved in fatty acid storage and glucose metabolis…

Designing metabolic engineering strategies with genome-scale metabolic flux modeling Open

Ryan S. Senger, Jiun Y. Yen, Imen Tanniche, Amanda Fisher, Glenda E. Gillaspy , et al. · 2015

New in silico tools that make use of genome-scale metabolic flux modeling are improving the design of metabolic engineering strategies. This review highlights the latest developments in this area, explains the interface between these in si…

Structural Basis of Phosphoinositide (PIP) Recognition by the TIRAP PIP-Binding Motif Open

Xiaolin Zhao, S. Y. Xiao, Sam Berk, Anne M. Brown, David R. Bevan , et al. · 2015

Influence of Sequence and Lipid Type on Membrane Perturbation by Human and Rat Amyloid β-Peptide (1-42) Open

Anne M. Brown, David R. Bevan · 2015

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