R. Haser
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Structural and functional studies of<i>Pseudomonas mesoacidophila</i>MX-45 trehalulose synthase and trehalulose hydrolase Open
SessionsC773 nicotinamide head of NADP + , as well as allowing the formation of a putative substrate binding pocket [2].These three structures have been instrumental in increasing our understanding of the mechanism, from a structural persp…
Fab′-induced folding of intrinsically disordered HIV-1 Tat Open
Poster Sessions C410 chain forming the beta-helices are very different.Nevertheless, gpV, gp138 and PA0616 are water soluble and SDS-resistant proteins and show no obvious membrane affinity.They are unlikely to unfold upon interaction with…
Roles of multiple surface sites, long substrate binding clefts, and carbohydrate binding modules in the action of amylolytic enzymes on polysaccharide substrates Open
Germinating barley seeds contain multiple forms of α-amylase, which are subject to both differential gene expression and differential degradation as part of the repertoire of starch-degrading enzymes. The α-amylases are endo-acting and pos…
Sugar-converting enzymes: new insights into structures and mechanisms Open
International audience
The ABC transporter BmrA from <i>Bacillus subtilis</i> is a functional dimer when in a detergent-solubilized state Open
BmrA from Bacillus subtilis is a half-size ABC (ATP-binding cassette) transporter involved in multidrug resistance. Although its supramolecular organization has been investigated after reconstitution in a lipid bilayer environment, and sho…
Crystallization and preliminary X-ray diffraction studies of two thermostable α-galactosidases from glycoside hydrolase family 36 Open
alpha-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The alpha-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus stearothermophilus have been overexpressed …
Structural study of the nucleolin~G-quartet complex Open
Bacteriophage replication initiator, known as O protein, is one of the two replication proteins encoded by the bacteriophage's own genome.Its role in initiating bacteriophage DNA replication has been firmly established.Crystals of O protei…
EED, a cellular partner of the viral proteins MA, IN and Nef from HIV-1 Open
MACROMOLECULES C236forms between the S atom of the catalytic residue Cys-145 of the enzyme and one of the epoxide carbon atoms of the peptide, thereby blocking the active site of the enzyme.With an appropriate sequence, the peptide also ha…
Carbohydrate recognition by α-amylase isozymes: their crystal structures reveal new binding sites and remarkable functional differences Open
International audience
Probing the Role of Divalent Metal Ions in a Bacterial Psychrophilic Metalloprotease: Binding Studies of an Enzyme in the Crystalline State by X-Ray Crystallography Open
The psychrophilic alkaline metalloprotease (PAP) produced by a Pseudomonas bacterium isolated in Antarctica belongs to the clan of metzincins, for which a zinc ion is essential for catalytic activity. Binding studies in the crystalline sta…
Amylose chain behavior in an interacting context II. Molecular modeling of a maltopentaose fragment in the barley ?-amylase catalytic site Open
In the first paper of this series, the tools necessary to evaluate the consequences of glucopyranose ring deformations in terms of glycosidic torsion angle shifts, and amylose chain propagation have been created. In this second paper, the …
Amylose chain behavior in an interacting context I. Influence of a nonchair ring on the maltose conformations Open
In the presence of steric constraints, flexible forms, i.e., skew (S), boat (B) or half-boat (H), were evoked from experimental data and conformational analyses by molecular mechanics calculations for glucopyranose rings of amylose fragmen…
Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor. Open
Alteromonas haloplanctis is a bacterium that flourishes in Antarctic sea-water and it is considered as an extreme psychrophile. We have determined the crystal structures of the alpha-amylase (AHA) secreted by this bacterium, in its native …
Crystallization and preliminary X-ray diffraction studies of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23. Open
A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the molecular basis of cold adaptations, cry…
Carbohydrate binding sites in a pancreatic alpha-amylase-substrate complex, derived from X-ray structure analysis at 2.1 A resolution. Open
The X-ray structure analysis of a crystal of pig pancreatic alpha-amylase (PPA, EC 3.2.1.1.) that was soaked with the substrate maltopentaose showed electron density corresponding to two independent carbohydrate recognition sites on the su…
Site-directed mutagenesis of histidine 93, aspartic acid 180, glutamic acid 205, histidine 290, and aspartic acid 291 at the active site and tryptophan 279 at the raw starch binding site in barley alpha-amylase 1. Open
The pseudotetrasaccharide acarbose has high affinity for the active site (Ki,app = 1 microM) and low affinity for a secondary site (Kd = 2.3 mM) in barley alpha-amylase 1, distinguished by inhibition kinetics and spectral perturbation. Mut…
Site-directed mutagenesis of tetraheme cytochrome c3. Modification of oxidoreduction potentials after heme axial ligand replacement. Open
The nature of the axial ligands of a heme group is an important factor in maintaining the oxidation-reduction potential of a c-type cytochrome. Cytochrome c3 from Desulfovibrio vulgaris Hildenborough contains four bis-histidinyl coordinate…
Crystallization of barley malt alpha-amylases and preliminary x-ray diffraction studies of the high pI isozyme, alpha-amylase 2. Open
alpha-Amylase isozymes 1 and 2 isolated from germinated barley seeds have been crystallized by the hanging- or sitting-drop vapor diffusion technique. Crystals of alpha-amylase 2 suitable for x-ray diffraction analysis were grown at pH 6.7…
Crystal structure and electron transfer properties of cytochrome c3. Open
The crystal structure of cytochrome c3 from the sulfate-reducing bacteria Desulfovibrio desulfuricans, Norway strain, has been determined through the fitting of the recently completed primary structure to a 2.5 A resolution electron densit…
Porcine pancreatic α-amylase: crystal structure and binding sites Open
STRUCTURAL MOLECULAR BIOLOGYsubstrates and inhibitors, namely, maltotriose, maltose, glucose and four kinds of iodine derivatives of substrates.These D-Fourier maps showed t~<o main binding sites I and I I. ~site II is at the bottom of the…