Raghu Kalluri
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View article: scPER: A Rigorous Computational Approach to Determine Cellular Subtypes in Tumors Aligned With Cancer Phenotypes From Total RNA Sequencing
scPER: A Rigorous Computational Approach to Determine Cellular Subtypes in Tumors Aligned With Cancer Phenotypes From Total RNA Sequencing Open
Single‐cell RNA sequencing (scRNA‐seq) is a powerful technique for understanding cellular diversity, but processing large patient cohorts to identify phenotype‐associated cell populations remains challenging. Here, scPER ( E stimating cell…
View article: Insights into the relevance of targeting fibroblasts to control cancer
Insights into the relevance of targeting fibroblasts to control cancer Open
Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) have garnered significant research attention in the last decade. As key stromal cells of the TME, studies have explored them as a potential target for controlling can…
View article: Engineered exosomes with KrasG12D specific siRNA in pancreatic cancer: a phase I study with immunological correlates
Engineered exosomes with KrasG12D specific siRNA in pancreatic cancer: a phase I study with immunological correlates Open
Oncogenic KRAS is amongst the key genetic drivers for initiation and maintenance of pancreatic ductal adenocarcinoma (PDAC). Here, we show that engineered exosomes with KrasG12D specific siRNA (iExoKrasG12D) reveal a biodistribution in pan…
View article: Oncogenic KRAS regulates secretion of extracellular vesicles and surface membrane charge via regulation of phosphatidylserine in pancreatic cancer
Oncogenic KRAS regulates secretion of extracellular vesicles and surface membrane charge via regulation of phosphatidylserine in pancreatic cancer Open
Extracellular vesicles (EVs) have the potential to be used as a liquid biopsy for cancer detection and treatment response assessment. Although the potential of EVs as disease-specific biomarkers has been promising, the rapid and specific e…
View article: Durotaxis is a driver and potential therapeutic target in lung fibrosis and metastatic pancreatic cancer
Durotaxis is a driver and potential therapeutic target in lung fibrosis and metastatic pancreatic cancer Open
Durotaxis, cell migration along stiffness gradients, is linked to embryonic development, tissue repair and disease. Despite solid in vitro evidence, its role in vivo remains largely speculative. Here we demonstrate that durotaxis actively …
View article: Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis
Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis Open
Extracellular vesicles (EVs) play a crucial role in intercellular communication, signaling pathways, and disease pathogenesis by transporting biomolecules such as DNA, RNA, proteins, and lipids derived from their cells of origin, and they …
View article: Suppression of BRCA1 facilitates kidney regeneration
Suppression of BRCA1 facilitates kidney regeneration Open
Maladaptive repair following kidney injury leads to the development of kidney disease. In this issue of JEM, Ajay et al. (https://doi.org/10.1084/jem.20231107) uncover the role of breast cancer susceptibility gene 1 (BRCA1) in cell cycle a…
View article: Advances in Extracellular Matrix-Associated Diagnostics and Therapeutics
Advances in Extracellular Matrix-Associated Diagnostics and Therapeutics Open
The extracellular matrix (ECM) is the common denominator of more than 50 chronic diseases. Some of these chronic pathologies lead to enhanced tissue formation and deposition, whereas others are associated with increased tissue degradation,…
View article: KRAS<sup>G12D</sup>-Specific Targeting with Engineered Exosomes Reprograms the Immune Microenvironment to Enable Efficacy of Immune Checkpoint Therapy in PDAC Patients
KRAS<sup>G12D</sup>-Specific Targeting with Engineered Exosomes Reprograms the Immune Microenvironment to Enable Efficacy of Immune Checkpoint Therapy in PDAC Patients Open
Oncogenic KRAS drives initiation and maintenance of pancreatic ductal adenocarcinoma (PDAC). Here, we show that engineered exosomes with Kras G12D specific siRNA (iExoKras G12D ) reveal impressive biodistribution in pancreas with negligibl…
View article: Distinct small molecule inhibitors of Kras specifically prime CTLA4 blockade therapy to transcriptionally reprogram Tregs and overcome resistance to suppress pancreas cancer
Distinct small molecule inhibitors of Kras specifically prime CTLA4 blockade therapy to transcriptionally reprogram Tregs and overcome resistance to suppress pancreas cancer Open
Lack of sustained response to oncogenic Kras (Kras*) inhibition in preclinical models and patients with pancreatic ductal adenocarcinoma (PDAC) emphasizes the need to identify impactful synergistic combination therapies to achieve robust c…
View article: CDK8 remodels the tumor microenvironment to resist the therapeutic efficacy of targeted KRAS<sup>G12D</sup> inhibition in pancreatic ductal adenocarcinoma
CDK8 remodels the tumor microenvironment to resist the therapeutic efficacy of targeted KRAS<sup>G12D</sup> inhibition in pancreatic ductal adenocarcinoma Open
Mutations in KRAS are a dominant driver of pancreatic ductal adenocarcinoma (PDAC), with over 40% of PDAC patients presenting with KRAS G12D mutations. The recent development of small molecule inhibitors targeting KRAS G12D has enabled tar…
View article: Genetics and biology of pancreatic ductal adenocarcinoma
Genetics and biology of pancreatic ductal adenocarcinoma Open
Pancreatic ductal adenocarcinoma (PDAC) poses a grim prognosis for patients. Recent multidisciplinary research efforts have provided critical insights into its genetics and tumor biology, creating the foundation for rational development of…
View article: High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography
High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography Open
Extracellular vesicles (EVs) have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures. Extracellular vesicles also offer advantages compared to synthetic vesicles for delivery of various drugs; ho…
View article: Type I conventional dendritic cells facilitate immunotherapy in pancreatic cancer
Type I conventional dendritic cells facilitate immunotherapy in pancreatic cancer Open
Inflammation and tissue damage associated with pancreatitis can precede or occur concurrently with pancreatic ductal adenocarcinoma (PDAC). We demonstrate that in PDAC coupled with pancreatitis (ptPDAC), antigen-presenting type I conventio…
View article: Genetic reprogramming with stem cells regenerates glomerular epithelial podocytes in Alport syndrome
Genetic reprogramming with stem cells regenerates glomerular epithelial podocytes in Alport syndrome Open
Glomerular filtration relies on the type IV collagen (ColIV) network of the glomerular basement membrane, namely, in the triple helical molecules containing the α3, α4, and α5 chains of ColIV. Loss of function mutations in the genes encodi…
View article: A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes
A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes Open
Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that ar…
View article: Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity
Development of an engineered extracellular vesicles-based vaccine platform for combined delivery of mRNA and protein to induce functional immunity Open
mRNA incorporated in lipid nanoparticles (LNPs) became a new class of vaccine modality for induction of immunity against COVID-19 and ushered in a new era in vaccine development. Here, we report a novel, easy-to-execute, and cost effective…
View article: Author Response: A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes
Author Response: A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes Open
Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that ar…
View article: A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes
A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes Open
Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that ar…
View article: Reviewer #1 (Public Review): A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes
Reviewer #1 (Public Review): A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes Open
Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that ar…
View article: Reviewer #2 (Public Review): A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes
Reviewer #2 (Public Review): A novel machine learning algorithm selects proteome signature to specifically identify cancer exosomes Open
Non-invasive early cancer diagnosis remains challenging due to the low sensitivity and specificity of current diagnostic approaches. Exosomes are membrane-bound nanovesicles secreted by all cells that contain DNA, RNA, and proteins that ar…
View article: Single extracellular vesicle imaging and computational analysis identifies inherent architectural heterogeneity
Single extracellular vesicle imaging and computational analysis identifies inherent architectural heterogeneity Open
Evaluating the heterogeneity of extracellular vesicles (EVs) is crucial for unraveling their complex actions and biodistribution. Here, we identify consistent architectural heterogeneity of EVs using cryogenic transmission electron microsc…
View article: Engineered immunomodulatory extracellular vesicles derived from epithelial cells acquire capacity for positive and negative T cell co-stimulation in cancer and autoimmunity
Engineered immunomodulatory extracellular vesicles derived from epithelial cells acquire capacity for positive and negative T cell co-stimulation in cancer and autoimmunity Open
Extracellular vesicles (EVs) are generated by all cells and systemic administration of allogenic EVs derived from epithelial and mesenchymal cells have been shown to be safe, despite carrying an array of functional molecules, including tho…