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View article: In silico interaction analysis of abscisic acid receptors in Zea mays: Insights into stress tolerance mechanisms
In silico interaction analysis of abscisic acid receptors in Zea mays: Insights into stress tolerance mechanisms Open
Harsh climates, mechanical injuries, and pathogen infection inflict significant loss to crop productivity, necessitating the development of more resilient agricultural varieties. The phytohormone Abscisic acid (ABA) plays a crucial role in…
View article: Oxidized dextran coated magnetic nanoparticles to develop magnetic cross-linked Bacillus lehensis G1 endolevanase aggregates for levan-type fructooligosaccharides synthesis
Oxidized dextran coated magnetic nanoparticles to develop magnetic cross-linked Bacillus lehensis G1 endolevanase aggregates for levan-type fructooligosaccharides synthesis Open
Endolevanase from Bacillus lehensis G1 (rlevblg1) is an enzyme that hydrolyzes levan for the production of levan-type fructooligosaccharides (L-FOS). To maximize the industrial utility of enzymes, it is essential to find effective immobili…
View article: In silico sequential mutagenesis of the Carbohydrate Binding Module Family 32 (CBM32) enhances ligand binding affinity
In silico sequential mutagenesis of the Carbohydrate Binding Module Family 32 (CBM32) enhances ligand binding affinity Open
Alginate lyase is a promising target for genetic modification for its degrading biofilm, contributing to bacterial proliferation and antimicrobial resistance. Apart from the main enzyme, the carbohydrate binding module (CBM) component can …
View article: Exploring The Protease Diversity of Psychrophilic Yeast, Glaciozyma antarctica Through Genome Mining Analysis
Exploring The Protease Diversity of Psychrophilic Yeast, Glaciozyma antarctica Through Genome Mining Analysis Open
Proteases are one of the most significant classes of enzymes, holding immense physiological relevance and extensive industrial applications. The genome of Glaciozyma antarctica was fully sequenced, showing 7,857 open reading frames that of…
View article: Strategy in Synthesizing Longer-Chain Levan-Type Fructooligosaccharides by Selective Dextran Macromolecular Cross-Linked Bacillus lehensis G1 Endolevanase Aggregate Immobilization
Strategy in Synthesizing Longer-Chain Levan-Type Fructooligosaccharides by Selective Dextran Macromolecular Cross-Linked Bacillus lehensis G1 Endolevanase Aggregate Immobilization Open
The formation of cross-linked enzyme aggregates (CLEAs) using macromolecular cross-linkers improves substrate accessibility and enhances enzyme retention. However, there have been few studies exploring the use of macromolecular cross-linke…
View article: PROTEIN INTERACTION STUDIES OF CROSS-LINKED ENDOLEVANASE AGGREGATES FROM BACILLUS LEHENSIS G1
PROTEIN INTERACTION STUDIES OF CROSS-LINKED ENDOLEVANASE AGGREGATES FROM BACILLUS LEHENSIS G1 Open
The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecu…
View article: Characterization of Maltogenic Amylase Activity Recovery: A Potential Approach for Improving Immobilization
Characterization of Maltogenic Amylase Activity Recovery: A Potential Approach for Improving Immobilization Open
Cross-linked enzymes aggregate (CLEA) is a versatile carrier free-immobilization technique that has gained much attention in the development of biocatalyst technology. However, there is no precise and accurate method of using this techniqu…
View article: IN-SITU FABRICATION OF FUNCTIONALIZED STARCH MAGNETIC NANOPARTICLES FOR IMMOBILIZATION OF LACCASE
IN-SITU FABRICATION OF FUNCTIONALIZED STARCH MAGNETIC NANOPARTICLES FOR IMMOBILIZATION OF LACCASE Open
Surface chemistry of magnetic nanoparticles (MNP) is crucial to provide a strong protein-support interaction for the immobilization process. The stability and biocompatibility of the MNP can be structurally enhanced by integrating with org…
View article: A Brief Review of Immobilized Oxidoreductase Enzymes for the Removal of Endocrine-Disrupting Chemicals from Wastewater
A Brief Review of Immobilized Oxidoreductase Enzymes for the Removal of Endocrine-Disrupting Chemicals from Wastewater Open
Modern technological of human activities in industries or housing areas have created an unhealthy environment, particularly through unmanageable wastewater. For the time being, this kind of pollution is getting serious as it caused the eme…
View article: Molecular and Interactions Modelling of PETase and Its Variant with Different Types of Crosslinker in Enzyme Immobilization
Molecular and Interactions Modelling of PETase and Its Variant with Different Types of Crosslinker in Enzyme Immobilization Open
Plastics are made from non-renewable resources and due to the tremendous production of plastics nowadays, they can lead to high levels of pollution. Biodegradation of plastic by utilizing enzymatic catalytic reaction is an environm…
View article: Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics
Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics Open
The biofibre complex structure in pineapple leaf fibres (PALFs) can be disrupted using the alkaline pre-treatment method with sodium hydroxide (NaOH). Nonetheless, the pre-treated structures have an impact on the bioconversion of PALFs int…
View article: Adsorptive Membrane for Boron Removal: Challenges and Future Prospects
Adsorptive Membrane for Boron Removal: Challenges and Future Prospects Open
The complexity of removing boron compounds from aqueous systems has received serious attention among researchers and inventors in the water treating industry. This is due to the higher level of boron in the aquatic ecosystem, which is caus…
View article: Biochemical and Physical Characterization of Immobilized Candida rugosa Lipase on Metal Oxide Hybrid Support
Biochemical and Physical Characterization of Immobilized Candida rugosa Lipase on Metal Oxide Hybrid Support Open
Enzyme immobilization on inorganic materials is gaining more attention with the potential characteristics of high-surface-area-to-volume ratios, increasing the efficiency of enzyme loading on the support. Metal oxide hybrid support was pre…
View article: Immobilization of maltogenic amylase in alginate-chitosan beads for improved enzyme retention and stability
Immobilization of maltogenic amylase in alginate-chitosan beads for improved enzyme retention and stability Open
Maltogenic amylase (Mag1) is a potent enzyme that hydrolyzes the glycosidic bond of polysaccharides to produce malto-oligosaccharides (MOS). However, the Mag1 enzyme has poor stability and reusability, leading to inefficient MOS production…
View article: Optimization and characterization of immobilized E. coli for engineered thermostable xylanase excretion and cell viability
Optimization and characterization of immobilized E. coli for engineered thermostable xylanase excretion and cell viability Open
There are many parameters that may have influenced the properties of cell during immobilization process. Particularly, the immobilization methods, carrier materials, and enzyme loading amount that have been proved to be important for immob…
View article: A review on immobilization strategy of cyclodextrin glucanotransferase producing Escherichia coli as whole cell biocatalyst
A review on immobilization strategy of cyclodextrin glucanotransferase producing Escherichia coli as whole cell biocatalyst Open
This paper reviewed the immobilization strategy on cyclodextrin glucanotransferase (CGTase) producing Escherichia coli as whole cell biocatalyst. Biotransformation of insoluble substrate such as starch and lignocellulosic biomass with whol…
View article: Optimization of Medium Fermentation for Isobutanol Production in Saccharomyces cerevisiae using Response Surface Methodology
Optimization of Medium Fermentation for Isobutanol Production in Saccharomyces cerevisiae using Response Surface Methodology Open
Biofuel is known to be a cleaner and sustainable fuel compared to fossil fuels making it more attractive to be used as transportation fuel. Isobutanol, a four-carbon alcohol has a range of physical properties that are more suitable to be u…
View article: Enzyme Kinetics Study for Heterogeneous System of Pretreated Kenaf Hydrolysis
Enzyme Kinetics Study for Heterogeneous System of Pretreated Kenaf Hydrolysis Open
The peculiarity of spatially restricted diffusion and molecular collision processes results in considerable contrast in a reaction between the reactant and catalyst in the heterogeneous system from its corresponding homogeneous structure. …
View article: Effect of Tryptone Concentration on Cyclodextrin Glucanotranferase (CGTase) Excretion and Cell Lysis of Immobilized Recombinant Escherichia coli
Effect of Tryptone Concentration on Cyclodextrin Glucanotranferase (CGTase) Excretion and Cell Lysis of Immobilized Recombinant Escherichia coli Open
The recombinant enzyme excretion into the culture medium provides significant advantages over cytoplasmic expression. Nevertheless, the problems encountered during the excretion of recombinant enzyme are the plasmid instability and occurre…
View article: Entrapment of Volvariella Volvacea Spores in Electrospun Nanofibers
Entrapment of Volvariella Volvacea Spores in Electrospun Nanofibers Open
Nanofibers containing Volariella volvacea spores were prepared by electrospinning technique. It has been one of the promising and alternative techniques in immobilizing microorganism cells including bacteria, virus and fungi through entrap…
View article: D-Xylonic Acid from Recombinant E. coli BL21 (DE3): Comparison between Shake Flask and Benchtop Bioreactor Fermentation
D-Xylonic Acid from Recombinant E. coli BL21 (DE3): Comparison between Shake Flask and Benchtop Bioreactor Fermentation Open
Production D -xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The com…
View article: BIOCONVERSION OF STARCH TO MALTOOLIGOSACCHARIDES (MOS) BY THE REACTION OF MALTOGENIC AMYLASE
BIOCONVERSION OF STARCH TO MALTOOLIGOSACCHARIDES (MOS) BY THE REACTION OF MALTOGENIC AMYLASE Open
Maltogenic amylase is one of the significant enzymes in oligosaccharides synthesis. Its ability to utilise multiple substrates and catalyse hydrolysis and transglycosylation reactions simultaneously makes it a unique biocatalyst. The catal…
View article: Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21
Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21 Open
Kenaf ( Hibiscus cannabinus L .), a potential fibre crop with a desirably high growth rate, could serve as a sustainable feedstock in the production of xylitol. In this work, the extraction of soluble products of kenaf through dilute nitri…
View article: IN SILICO STRUCTURAL CHARACTERIZATION OF L. lactis subsp. cremoris MG1363 FFH-FTSY COMPLEX IN PROTEIN TARGETING INTERACTION
IN SILICO STRUCTURAL CHARACTERIZATION OF L. lactis subsp. cremoris MG1363 FFH-FTSY COMPLEX IN PROTEIN TARGETING INTERACTION Open
In bacteria, gene conservation and experimental data show that Lactococcus lactis has the simplest version of protein secretion system compared to Escherichia coli and Bacillus subtilis whose systems are more complex. L. lactis only posses…
View article: Enhancement of Isobutanol and 3-Methyl-1-Butanol Production Yields in Saccharomyces Cerevisiae without Genetic Modification
Enhancement of Isobutanol and 3-Methyl-1-Butanol Production Yields in Saccharomyces Cerevisiae without Genetic Modification Open
Bio-based fuel produced from the renewable resources is efficiently overcome the shortcomings of fossil fuels. Several factors such as the increasing awareness on environmental problems, fossil fuel prices and the sustainability of energy …
View article: Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention Open
This work aimed to optimize ten preparation factors that might influence the cellulase and xylanase activity retention of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica. The factors were optimized …
View article: Assessment of microwave-assisted pretreatments for enhancing pineapple waste delignification
Assessment of microwave-assisted pretreatments for enhancing pineapple waste delignification Open
Biological degradation of biomass for the production of fine chemicals is getting much interest nowadays. However, the complex and recalcitrance structure of the biomass hinders the success story of the degradation. Lignin is the main comp…
View article: OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION
OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION Open
Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using N…
View article: Pengenalpastian dan Profil Pengekspresan Gen Biosintesis Asid Amino Yis Psikrofil, Glaciozyma Antarctica
Pengenalpastian dan Profil Pengekspresan Gen Biosintesis Asid Amino Yis Psikrofil, Glaciozyma Antarctica Open
The mechanism of amino acid uptake and synthesis in the psychrophilic microorganism lives and proliferate in the extreme low-temperature environment is still not well understood.The aim of this study was to identify genes involved in amino…
View article: Cellobiohydrolase B of <i>Aspergillus niger</i> over-expressed in <i>Pichia pastoris</i> stimulates hydrolysis of oil palm empty fruit bunches
Cellobiohydrolase B of <i>Aspergillus niger</i> over-expressed in <i>Pichia pastoris</i> stimulates hydrolysis of oil palm empty fruit bunches Open
Background Aspergillus niger , along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-gluc…