Rainer Cramer
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View article: Rapid and accurate biological sex estimation by LAP-MALDI MS analysis of child teeth
Rapid and accurate biological sex estimation by LAP-MALDI MS analysis of child teeth Open
Accurate biological sex estimation using child teeth was achieved for the first time by MALDI MS(/MS) analysis, within 10 min per tooth using inexpensive consumables and peptide extraction by mild acid etching of the enamel.
View article: LAP-MALDI MS analysis of amelogenin from teeth for biological sex estimation
LAP-MALDI MS analysis of amelogenin from teeth for biological sex estimation Open
The biological sex estimation of human individuals can be achieved by extracting fragments of the amelogenin protein from small areas of tooth enamel. The amelogenin gene can be found on both sex chromosomes (X and Y) with chromosome-speci…
View article: Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using a Commercial Ion Source and Orbitrap Mass Analyzer
Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using a Commercial Ion Source and Orbitrap Mass Analyzer Open
A liquid atmospheric pressure-matrix-assisted laser desorption/ionization (LAP-MALDI) method has been developed and applied to a commercial AP-MALDI source on a hybrid orbitrap mass analyzer. It is shown that electrospray ionization (ESI)-…
View article: Detection of Antimicrobial Proteins/Peptides and Bacterial Proteins Involved in Antimicrobial Resistance in Raw Cow’s Milk from Different Breeds
Detection of Antimicrobial Proteins/Peptides and Bacterial Proteins Involved in Antimicrobial Resistance in Raw Cow’s Milk from Different Breeds Open
Proteins involved in antibiotic resistance (resistome) and with antimicrobial activity are present in biological specimens. This study aims to explore the presence and abundance of antimicrobial peptides (AMPs) and resistome proteins in bo…
View article: Development and translation of a method of clinical utility for LC-MS/MS analysis to detect SARS-CoV-2 antigens from ONP swabs and saliva
Development and translation of a method of clinical utility for LC-MS/MS analysis to detect SARS-CoV-2 antigens from ONP swabs and saliva Open
During the COVID-19 pandemic, development of diagnostic tests was vital to chart the course and to reduce the impact of the infection. Continued testing and surveillance of vaccine escape will continue for years to come which presents an o…
View article: LAP-MALDI MS Profiling and Identification of Potential Biomarkers for the Detection of Bovine Tuberculosis
LAP-MALDI MS Profiling and Identification of Potential Biomarkers for the Detection of Bovine Tuberculosis Open
Detecting bovine tuberculosis (bTB) primarily relies on the tuberculin skin test, requiring two separate animal handling events with a period of incubation time (normally 3 days) between them. Here, we present the use of liquid atmospheric…
View article: The use of salts, buffers and surfactants in LAP-MALDI MS
The use of salts, buffers and surfactants in LAP-MALDI MS Open
Biological samples such as tissue extracts and enzymatic assays typically have a complex composition, which can interfere with analyte ionisation and detection in mass spectrometry (MS). Ionisation techniques such as electrospray ionisatio…
View article: Ultra-High-Throughput and Low-Volume Analysis of Intact Proteins with LAP-MALDI MS
Ultra-High-Throughput and Low-Volume Analysis of Intact Proteins with LAP-MALDI MS Open
High-throughput (HTP) mass spectrometry (MS) is a rapidly growing field, with many techniques evolving to accommodate ever increasing sample analysis rates. Many of these techniques, such as AEMS and IR-MALDESI MS, require volumes of at le…
View article: Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Open
Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
View article: Data from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
Data from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Open
The promising antitumor activity of 17-allylamino-17-demethoxygeldanamycin (17AAG) results from inhibition of the molecular chaperone heat shock protein 90 (HSP90) and subsequent degradation of multiple oncogenic client proteins. Gene expr…
View article: Supplementary Figure 1 from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
Supplementary Figure 1 from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Open
Supplementary Figure 1 from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
View article: Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Open
Supplementary Data URL from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
View article: Data from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin
Data from Gene and Protein Expression Profiling of Human Ovarian Cancer Cells Treated with the Heat Shock Protein 90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Open
The promising antitumor activity of 17-allylamino-17-demethoxygeldanamycin (17AAG) results from inhibition of the molecular chaperone heat shock protein 90 (HSP90) and subsequent degradation of multiple oncogenic client proteins. Gene expr…
View article: Advances in ionisation techniques for mass spectrometry‐based omics research
Advances in ionisation techniques for mass spectrometry‐based omics research Open
Omics analysis by mass spectrometry (MS) is a vast field, with proteomics, metabolomics and lipidomics dominating recent research by exploiting biological MS ionisation techniques. Traditional MS ionisation techniques such as electrospray …
View article: Ultrahigh-Throughput Sample Analysis Using Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Ultrahigh-Throughput Sample Analysis Using Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Open
Mass spectrometry (MS) allows for automated analysis of complex samples at high resolution without the need for labeling/derivatization. Liquid atmospheric pressure matrix-assisted laser desorption/ionization (LAP-MALDI) enables rapid samp…
View article: LAP-MALDI MS coupled with machine learning: an ambient mass spectrometry approach for high-throughput diagnostics
LAP-MALDI MS coupled with machine learning: an ambient mass spectrometry approach for high-throughput diagnostics Open
LAP-MALDI MS profiling provides rapid, cost-effective large-scale disease analysis as demonstrated by preclinical detection of bovine mastitis and antimicrobial resistance testing using a longitudinal sample collection from a 500-cows dair…
View article: Speciation and milk adulteration analysis by rapid ambient liquid MALDI mass spectrometry profiling using machine learning
Speciation and milk adulteration analysis by rapid ambient liquid MALDI mass spectrometry profiling using machine learning Open
Growing interest in food quality and traceability by regulators as well as consumers demands advances in more rapid, versatile and cost-effective analytical methods. Milk, as most food matrices, is a heterogeneous mixture composed of metab…
View article: Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR)
Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR) Open
The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expressio…
View article: Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis
Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis Open
Rapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the ill…
View article: Advancing Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Toward Ultrahigh-Throughput Analysis
Advancing Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Toward Ultrahigh-Throughput Analysis Open
Label-free high-throughput screening using mass spectrometry has the potential to provide rapid large-scale sample analysis at a speed of more than one sample per second. Such speed is important for compound library, assay and future clini…
View article: Peptide nanotubes self-assembled from leucine-rich alpha helical surfactant-like peptides
Peptide nanotubes self-assembled from leucine-rich alpha helical surfactant-like peptides Open
The designed arginine-rich surfactant-like peptide R3L12 (arginine3–leucine12) is shown to form diversity of self-assembled nanostructures in aqueous solution, depending on pH, including nanotubes, tubular networks in 3D and square planar …
View article: Speciation and adulteration analysis of milk by liquid AP-MALDI MS profiling
Speciation and adulteration analysis of milk by liquid AP-MALDI MS profiling Open
AP-MALDI MS profiling coupled with multivariate analysis for speciation and the detection of food adulteration using a simple one-pot sample preparation of milk. Milk samples from cow, goat, sheep and camel were classified with 100% accura…
View article: Atmospheric Pressure Ultraviolet Laser Desorption and Ionization from Liquid Samples for Native Mass Spectrometry
Atmospheric Pressure Ultraviolet Laser Desorption and Ionization from Liquid Samples for Native Mass Spectrometry Open
Understanding protein structure is vital for evaluating protein interactions with drugs, proteins, and other ligands. Native mass spectrometry (MS) is proving to be invaluable for this purpose, enabling analysis of "native-like" samples th…
View article: Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Adds Enhanced Functionalities to MALDI MS Profiling for Disease Diagnostics
Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Adds Enhanced Functionalities to MALDI MS Profiling for Disease Diagnostics Open
A liquid matrix-assisted laser desorption/ionization (liquid MALDI) method has been developed for high-throughput atmospheric pressure (AP) mass spectrometry (MS) analysis of the molecular content of crude bioliquids for disease diagnostic…
View article: Dataset to support AP-MALDI native ion mobility-mass spectrometry using liquid samples.
Dataset to support AP-MALDI native ion mobility-mass spectrometry using liquid samples. Open
The dataset contains ion mobility-mass spectrometry data used for figures in the article, 'Atmospheric pressure ultraviolet laser desorption and ionization from liquid samples for native mass spectrometry', submitted for review in Summer 2…
View article: Dataset to support lipid profiling of bacteria using liquid AP-MALDI MS
Dataset to support lipid profiling of bacteria using liquid AP-MALDI MS Open
This dataset contains mass spectrometry raw data files, multivariate models and cross-validation reports for principle component analysis. The samples used for data acquisition were bacterial extracts from 10 species including C. jejuni, E…
View article: Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix‐assisted laser desorption/ionization mass spectrometry
Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix‐assisted laser desorption/ionization mass spectrometry Open
Rationale Liquid atmospheric pressure matrix‐assisted laser desorption/ionisation (AP‐MALDI) has been shown to enable the production of electrospray ionisation (ESI)‐like multiply charged analyte ions with little sample consumption and lon…