Raja Sekhar Nirujogi
YOU?
Author Swipe
View article: Molecular profiling of brain endothelial cell to astrocyte endfoot communication in mouse and human
Molecular profiling of brain endothelial cell to astrocyte endfoot communication in mouse and human Open
Our understanding of how the body communicates with the brain to coordinate their functions is remarkably limited. At the blood-brain barrier (BBB), brain endothelial cells (BECs) are ideally positioned to mediate signaling between blood a…
View article: Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved peptide motif
Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved peptide motif Open
Inherited mutations in VPS35 and LRRK2 kinase lead to hyperphosphorylation of Rab GTPases. RH2 domain-containing proteins from the RILP homology family, such as RILPL1, are Rab effectors that recognize the LRRK2-phosphorylated switch 2 thr…
View article: TDP-43 loss of function drives aberrant splicing in Parkinson’s disease
TDP-43 loss of function drives aberrant splicing in Parkinson’s disease Open
Introductory paragraph While mRNA splicing dysregulation is a well-established contributor to neurodegeneration in disorders such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), its role in Parkinson’s disease (PD…
View article: Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif
Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif Open
SUMMARY Inherited mutations in VPS35 and the kinase LRRK2 lead to hyperphosphorylation of Rab GTPases and promote the formation of phospho-Rab signalling complexes. A subset of RH2 domain-containing proteins from the RILP-homology family, …
View article: <i>C9orf72</i>polyGA knock-in mice exhibit mild motor and proteomic changes consistent with ALS/FTD
<i>C9orf72</i>polyGA knock-in mice exhibit mild motor and proteomic changes consistent with ALS/FTD Open
A GGGGCC repeat expansion in C9orf72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The repeat expansion is translated into five different dipeptide repeat proteins: polyGA, polyG…
View article: Cellular and Extracellular microRNA Dysregulation in LRRK2-Linked Parkinson’s Disease
Cellular and Extracellular microRNA Dysregulation in LRRK2-Linked Parkinson’s Disease Open
Background and objective The discovery of cell-free micro-RNAs in body fluids has made them a promising biomarker target in the field of neurodegenerative diseases. Although they have been reported to be differentially expressed in bioflui…
View article: Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples
Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples Open
Lysosomes are implicated in a wide spectrum of human diseases, including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration, and cancer. Profiling lysosomal content using tag-based lysosomal immunoprecipitation …
View article: In-depth mass-spectrometry reveals phospho-RAB12 as a blood biomarker of G2019S LRRK2-driven Parkinson’s disease
In-depth mass-spectrometry reveals phospho-RAB12 as a blood biomarker of G2019S LRRK2-driven Parkinson’s disease Open
Leucine-rich repeat kinase 2 (LRRK2) inhibition is a promising disease-modifying therapy for LRRK2-associated Parkinson’s disease (L2PD) and idiopathic PD. However, pharmacodynamic readouts and progression biomarkers for clinical trials ai…
View article: Variants in the proteasome regulator PSMF1 cause a phenotypic spectrum from early-onset Parkinson’s disease to perinatal lethality and disrupt mitochondrial function
Variants in the proteasome regulator PSMF1 cause a phenotypic spectrum from early-onset Parkinson’s disease to perinatal lethality and disrupt mitochondrial function Open
Dissecting biological pathways highlighted by Mendelian gene discovery has provided critical insights into the pathogenesis of Parkinson’s disease (PD) and neurodegeneration. This approach ultimately catalyzes the identification of potenti…
View article: Tagless LysoIP method for molecular profiling of lysosomal content in clinical samples
Tagless LysoIP method for molecular profiling of lysosomal content in clinical samples Open
Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling lysosomal content using tag-based lysosomal immunoprecipitation (L…
View article: In-depth mass-spectrometry reveals phospho-RAB12 as a blood biomarker of G2019S LRRK2-driven Parkinson’s
In-depth mass-spectrometry reveals phospho-RAB12 as a blood biomarker of G2019S LRRK2-driven Parkinson’s Open
Leucine-rich repeat kinase 2 (LRRK2) inhibition is a promising disease-modifying therapy for LRRK2-associated Parkinson’s disease (L2PD) and idiopathic PD (iPD). Yet, pharmaco-dynamic readouts and progression biomarkers for disease modific…
View article: Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies Open
[This corrects the article DOI: 10.1371/journal.pone.0253364.].
View article: CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data
CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data Open
To facilitate analysis and sharing of mass spectrometry (MS)-based proteomics data, we created online tools called CURTAIN ( https://curtain.proteo.info ) and CURTAIN-PTM ( https://curtainptm.proteo.info ) with an accompanying series of vi…
View article: Parkinson’s VPS35[D620N] mutation induces LRRK2-mediated lysosomal association of RILPL1 and TMEM55B
Parkinson’s VPS35[D620N] mutation induces LRRK2-mediated lysosomal association of RILPL1 and TMEM55B Open
We demonstrate that the Parkinson’s VPS35[D620N] mutation alters the expression of ~220 lysosomal proteins and stimulates recruitment and phosphorylation of Rab proteins at the lysosome. This recruits the phospho-Rab effector protein RILPL…
View article: CURTAIN – A Unique Web-based tool for exploration and sharing of MS-based proteomics data
CURTAIN – A Unique Web-based tool for exploration and sharing of MS-based proteomics data Open
To facilitate analysis and sharing of mass spectrometry (MS)-based proteomics data we created tools called CURTAIN ( https://curtain.proteo.info ) and CURTAIN-PTM ( https://curtainptm.proteo.info ). These enable the non-MS expert to intera…
View article: PolyGR and polyPR knock-in mice reveal a conserved neuroprotective extracellular matrix signature in<i>C9orf72</i>ALS/FTD neurons
PolyGR and polyPR knock-in mice reveal a conserved neuroprotective extracellular matrix signature in<i>C9orf72</i>ALS/FTD neurons Open
A GGGGCC repeat expansion in C9orf72 is the most common genetic cause of ALS and FTD (C9ALS/FTD). The presence of dipeptide repeat (DPR) proteins, generated by translation of the expanded repeat, is a major pathogenic feature of C9ALS/FTD …
View article: Whole proteome copy number dataset in primary mouse cortical neurons
Whole proteome copy number dataset in primary mouse cortical neurons Open
The functional diversity of neurons is specified through their proteome resulting in elaborate and tightly regulated protein interaction networks and signalling that regulates neuronal processes. Dysregulation of these dynamic networks in …
View article: Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons
Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons Open
Raw data-Supplementary table- "Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons"
View article: Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons
Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons Open
Raw data-Supplementary table- "Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons"
View article: Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B
Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B Open
The Parkinson’s VPS35[D620N] mutation causes lysosome dysfunction enhancing LRRK2 kinase activity. We find the VPS35[D620N] mutation alters expression of ∼350 lysosomal proteins and stimulates LRRK2 phosphorylation of Rab proteins at the l…
View article: Golgi-IP, a tool for multimodal analysis of Golgi molecular content
Golgi-IP, a tool for multimodal analysis of Golgi molecular content Open
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emer…
View article: Parkinson's VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B
Parkinson's VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B Open
Confocoal microscopy images, CellProfiler excel files of Pearson's coefficients between TMEM55B/RILPL1 or p.Rab10/RILPL1 in R1441C MEF or VPS35[D620N] MEF cells, and prism files of graphed Pearson's coefficients for each condition, as seen…
View article: Cell-autonomous immune dysfunction driven by disrupted autophagy in <i>C9orf72</i> -ALS iPSC-derived microglia contributes to neurodegeneration
Cell-autonomous immune dysfunction driven by disrupted autophagy in <i>C9orf72</i> -ALS iPSC-derived microglia contributes to neurodegeneration Open
Although microglial activation is widely found in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), the underlying mechanism(s) are poorly understood. Here, using human-induced pluripotent stem cell–derived microglia-l…
View article: Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content
Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content Open
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emer…
View article: PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain
PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain Open
Leucine-rich-repeat-kinase 1 (LRRK1) and its homolog LRRK2 are multidomain kinases possessing a ROC-CORA-CORB containing GTPase domain and phosphorylate distinct Rab proteins. LRRK1 loss of function mutations cause the bone disorder osteos…
View article: Discovery of XL01126: A Potent, Fast, Cooperative, Selective, Orally Bioavailable, and Blood–Brain Barrier Penetrant PROTAC Degrader of Leucine-Rich Repeat Kinase 2
Discovery of XL01126: A Potent, Fast, Cooperative, Selective, Orally Bioavailable, and Blood–Brain Barrier Penetrant PROTAC Degrader of Leucine-Rich Repeat Kinase 2 Open
Leucine-rich repeat kinase 2 (LRRK2) is one of the most promising targets for Parkinson's disease. LRRK2-targeting strategies have primarily focused on type 1 kinase inhibitors, which, however, have limitations as the inhibited protein can…