Ramawatar Nagar
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Pier abutment: Bridge the gap with non rigid connector - A clinical case report Open
Pier abutment is defined as a freestanding abutment with edentulous space on both sides. This case report described the rehabilitation of a patient who presented to the department with the primary complaint of missing teeth and difficulty …
A Novel Technique for Retention of the Immediate Obturator Following Maxillectomy in Mucormycosis: A Case Report Open
Mucormycosis is an aggressive opportunistic fungal infection that affects blood supply-rich areas such as the maxilla. Because of the compromised immune system caused by coronavirus disease 2019 (COVID-19) infection and diabetes, this infe…
View article: A Systematic Phylogenomic Classification of the Multidrug and Toxic Compound Extrusion Transporter Gene Family in Plants
A Systematic Phylogenomic Classification of the Multidrug and Toxic Compound Extrusion Transporter Gene Family in Plants Open
Multidrug and toxic compound extrusion (MATE) transporters comprise a multigene family that mediates multiple functions in plants through the efflux of diverse substrates including organic molecules, specialized metabolites, hormones, and …
View article: A Chromosome Scale Assembly of an Australian <i>Puccinia striiformis</i> f. sp. <i>tritici</i> Isolate of the <i>PstS1</i> Lineage
A Chromosome Scale Assembly of an Australian <i>Puccinia striiformis</i> f. sp. <i>tritici</i> Isolate of the <i>PstS1</i> Lineage Open
Support was provided to S.Periyannan by the Australian Research Council (ARC) with funding through the Discovery Early Career Researcher Award (DE17010015) and to B. Schwessinger by the ARC Future Fellowship (FT180100024)
High-molecular weight DNA extraction and small fragment removal ofAscochyta lentis v1 Open
Extracting pure high-molecular weight DNA from some fungal species is difficult due to the presence of polysaccharides and potentially other compounds which biochemically mimic DNA or interfere with the DNA extraction process. Such compoun…
Distinct Life Histories Impact Dikaryotic Genome Evolution in the Rust Fungus Puccinia striiformis Causing Stripe Rust in Wheat Open
Stripe rust of wheat, caused by the obligate biotrophic fungus Puccinia striiformis f.sp. tritici, is a major threat to wheat production worldwide with an estimated yearly loss of US $1 billion. The recent advances in long-read sequencing …
Distinct life histories impact dikaryotic genome evolution in the rust fungus <i>Puccinia striiformis</i> causing stripe rust in wheat Open
Stripe rust of wheat, caused by the obligate biotrophic fungus Puccinia striiformis f. sp. tritici , is a major threat to wheat production world-wide with an estimated yearly loss of US $1 billion. The recent advances in long-read sequenci…
High-molecular weight DNA extraction from challenging fungi using CTAB and gel purification v2 Open
Extracting pure high-molecular weight DNA from some fungal species is difficult due to the presence of polysaccharides and potentially other compounds which biochemically mimic DNA or interfere with the DNA extraction process. Such compoun…
Harnessing the MinION: An example of how to establish long‐read sequencing in a laboratory using challenging plant tissue from <i>Eucalyptus pauciflora</i> Open
Long‐read sequencing technologies are transforming our ability to assemble highly complex genomes. Realizing their full potential is critically reliant on extracting high‐quality, high‐molecular‐weight (HMW) DNA from the organisms of inter…
Multi-step high purity high molecular weight DNA extraction protocol from challenging fungal tissues v1 Open
This protocol is based on the fact that we struggled for a long time to obtain high purity DNA from the fungal material, especially from several rust species. We finally thought we made it when we obtained DNA with perfect QC measures usin…
High purity, high molecular weight DNA extraction from rust spores via CTAB based DNA precipitation for long read sequencing v1 Open
Nanopore sequencing (Oxford Nanopore Technologies MinION instrument) requires high quality, high molecular weight DNA (HMW) to produce long sequence reads. Extracting high purity, HMW DNA is a difficult challenge, especially for a biotroph…
View article: Functional Characterization of Novel Chitinase Genes Present in the Sheath Blight Resistance QTL: qSBR11-1 in Rice Line Tetep
Functional Characterization of Novel Chitinase Genes Present in the Sheath Blight Resistance QTL: qSBR11-1 in Rice Line Tetep Open
Rice sheath blight disease caused by Rhizoctonia solani is one of the most devastating diseases in rice leading to heavy yield losses. Due to the polygenic nature of resistance, no major resistance gene with complete host resistance agains…