Ramkrishna Adhikary
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View article: A simplified hybrid capture approach retains high specificity and enables PCR-free workflow
A simplified hybrid capture approach retains high specificity and enables PCR-free workflow Open
We present a targeted sequencing workflow that eliminates bead-based capture, multiple washes, and post-hybridization PCR, while improving various aspects of data quality. The performance of the approach was evaluated by sequencing hundred…
View article: Direct Observation of Peptide Hydrogel Self-Assembly
Direct Observation of Peptide Hydrogel Self-Assembly Open
The characterization of self-assembling molecules presents significant experimental challenges, especially when associated with phase separation or precipitation. Transparent window infrared (IR) spectroscopy leverages site-specific probes…
View article: Direct observation of peptide hydrogel self-assembly
Direct observation of peptide hydrogel self-assembly Open
To facilitate the characterization of phase-transitioning molecules, site-specific non-perturbative infrared probes are leveraged for continuous observation of the self-assembly of fibrils in a peptide hydrogel following stopped-flow initi…
View article: Direct Observation of Peptide Hydrogel Self-Assembly
Direct Observation of Peptide Hydrogel Self-Assembly Open
The characterization of self-assembling molecules presents significant experimental challenges, especially when associated with phase separation or precipitation. Transparent window infrared (IR) spectroscopy leverages site-specific probes…
View article: New codons for efficient production of unnatural proteins in a semisynthetic organism
New codons for efficient production of unnatural proteins in a semisynthetic organism Open
View article: Structure and Dynamics of Stacking Interactions in an Antibody Binding Site
Structure and Dynamics of Stacking Interactions in an Antibody Binding Site Open
For years, antibodies (Abs) have been used as a paradigm for understanding how protein structure contributes to molecular recognition. However, with the ability to evolve Abs that recognize specific chromophores, they also have great poten…
View article: An Alternative Terminal Step of the General Secretory Pathway in Staphylococcus aureus
An Alternative Terminal Step of the General Secretory Pathway in Staphylococcus aureus Open
Type I signal peptidase (SPase) is essential for viability in wild-type bacteria because the terminal step of the bacterial general secretory pathway requires its proteolytic activity to release proteins from their membrane-bound N-termina…
View article: Adaptive Mutations Alter Antibody Structure and Dynamics during Affinity Maturation
Adaptive Mutations Alter Antibody Structure and Dynamics during Affinity Maturation Open
While adaptive mutations can bestow new functions on proteins via the introduction or optimization of reactive centers, or other structural changes, a role for the optimization of protein dynamics also seems likely but has been more diffic…