Raphaël Dutoit
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View article: Vsb1, Ypq1 and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole
Vsb1, Ypq1 and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole Open
Although the yeast vacuole plays a crucial role in storing and mobilizing cationic amino acids (CAA), CAA transport at the vacuolar membrane remains poorly understood. Here, by combining analysis of CAA pools, uptake and permeabilization a…
View article: Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis
Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis Open
The ability of retaining glycoside hydrolases (GHs) to transglycosylate is inherent to the double-displacement mechanism. Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could prov…
View article: <scp>M42</scp> aminopeptidase catalytic site: the structural and functional role of a strictly conserved aspartate residue
<span>M42</span> aminopeptidase catalytic site: the structural and functional role of a strictly conserved aspartate residue Open
The M42 aminopeptidases are a family of dinuclear aminopeptidases widely distributed in Prokaryotes. They are potentially associated to the proteasome, achieving complete peptide destruction. Their most peculiar characteristic is their qua…
View article: X-Ray Crystallography to Study the Oligomeric State Transition of the <em>Thermotoga maritima</em> M42 Aminopeptidase TmPep1050
X-Ray Crystallography to Study the Oligomeric State Transition of the <em>Thermotoga maritima</em> M42 Aminopeptidase TmPep1050 Open
The M42 aminopeptidases form functionally active complexes made of 12 subunits. Their assembly process appears to be regulated by their metal ion cofactors triggering a dimer-dodecamer transition. Upon metal ion binding, several structural…
View article: M42 aminopeptidase catalytic site: the structural and functional role of a strictly conserved aspartate residue
M42 aminopeptidase catalytic site: the structural and functional role of a strictly conserved aspartate residue Open
The M42 aminopeptidases are a family of dinuclear aminopeptidases widely distributed in Prokaryotes. They are potentially associated to the proteasome, achieving complete peptide destruction. Their most peculiar characteristic is their qua…
View article: Characterization of Putative Virulence Factors of <i>Pseudomonas aeruginosa</i> Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB
Characterization of Putative Virulence Factors of <i>Pseudomonas aeruginosa</i> Strain RBS Isolated from a Saltern, Tunisia: Effect of Metal Ion Cofactors on the Structure and the Activity of LasB Open
Pseudomonas aeruginosa is a ubiquitous Gram‐negative bacterium able to survive in diverse environments such as soil, plants, freshwater, and seawater. P. aeruginosa can be an opportunistic pathogen to humans when their immune system is def…
View article: Crystal structure determination of<i>Pseudomonas stutzeri</i>A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes
Crystal structure determination of<i>Pseudomonas stutzeri</i>A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes Open
The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes. In this study, recombinant Ps_Cel5A from Pseudomona…