Raphael Trösch
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Reverse genetics in the <i>Arabidopsis</i> chloroplast genome identifies <i>rps16</i> as a transcribed pseudogene Open
SUMMARY The plastid (chloroplast) genomes of seed plants contain a conserved set of ribosomal protein genes. The rps16 gene represents an exception: It has been lost from the plastid genomes of gymnosperms and several lineages of angiosper…
One-helix protein 2 is not required for the synthesis of photosystem II subunit D1 in Chlamydomonas Open
In land plants and cyanobacteria, co-translational association of chlorophyll (Chl) to the nascent D1 polypeptide, a reaction center protein of photosystem II (PSII), requires a Chl binding complex consisting of a short-chain dehydrogenase…
OHP2 is not required for <i>psbA</i> translation in <i>Chlamydomonas</i> Open
In land plants and cyanobacteria, co-translational association of chlorophyll (Chl) to the nascent D1 polypeptide, a reaction center protein of photosystem II (PSII), requires a Chl binding complex consisting of a short-chain dehydrogenase…
Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation Open
Photosynthesis is a central determinant of plant biomass production, but its homeostasis is increasingly challenged by heat. Little is known about the sensitive regulatory principles involved in heat acclimation that underly the biogenesis…
The versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry Open
In plant cells, chloroplast gene expression is predominantly controlled through post-transcriptional regulation. Such fine-tuning is vital for precisely orchestrating protein complex assembly as for the photosynthesis machinery and for qui…
The proteomic inventory reveals the chloroplast ribosome as nexus within a diverse protein network Open
Chloroplast gene expression is tightly regulated and majorly controlled on the level of protein synthesis. Fine-tuning of translation is vital for plant development, acclimation to environmental challenges and for the assembly of major pro…
The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants Open
Cells are highly adaptive systems that respond and adapt to changing environmental conditions such as temperature fluctuations or altered nutrient availability. Such acclimation processes involve reprogramming of the cellular gene expressi…
The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants Open
Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly of protein complexes, composed of both imported and locally synthesized subunits, are vi…
View article: Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga <i>Chlamydomonas reinhardtii</i>
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga <i>Chlamydomonas reinhardtii</i> Open
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factor…
Suppressors of the Chloroplast Protein Import Mutant <i>tic40</i> Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis Open
To extend our understanding of chloroplast protein import and the role played by the import machinery component Tic40, we performed a genetic screen for suppressors of chlorotic tic40 knockout mutant Arabidopsis thaliana plants. As a resul…