Ray Collier
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A key to totipotency: <i>Wuschel‐like homeobox 2a</i> unlocks embryogenic culture response in maize (<i>Zea mays</i> L.) Open
Summary The ability of plant somatic cells to dedifferentiate, form somatic embryos and regenerate whole plants in vitro has been harnessed for both clonal propagation and as a key component of plant genetic engineering systems. Embryogeni…
Role of ureides in source-to-sink transport of photoassimilates in non-fixing soybean Open
Nitrogen (N)-fixing soybean plants use the ureides allantoin and allantoic acid as major long-distance transport forms of N, but in non-fixing, non-nodulated plants amino acids mainly serve in source-to-sink N allocation. However, some ure…
A versatile and robust Agrobacterium‐based gene stacking system generates high‐quality transgenic Arabidopsis plants Open
Summary Biotechnology provides a means for the rapid genetic improvement of plants. Although single genes have been important in engineering herbicide and pest tolerance traits in crops, future improvements of complex traits like yield and…
Accurate measurement of transgene copy number in crop plants using droplet digital <span>PCR</span> Open
Summary Genetic transformation is a powerful means for the improvement of crop plants, but requires labor‐ and resource‐intensive methods. An efficient method for identifying single‐copy transgene insertion events from a population of inde…
Draft Genome Sequence of <i>Agrobacterium rhizogenes</i> Strain NCPPB2659 Open
This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 sc…
Use of Agrobacterium rhizogenes Strain 18r12v and Paromomycin Selection for Transformation of Brachypodium distachyon and Brachypodium sylvaticum Open
The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wi…