Rebecca Chandler-Bostock
YOU?
Author Swipe
View article: Visualizing Viral RNA Packaging Signals in Action
Visualizing Viral RNA Packaging Signals in Action Open
Here we confirm, using genome-scale RNA fragments in assembly competition assays, that multiple sub-sites (Packaging Signals, PSs) across the 5' two-thirds of the gRNA of Satellite Tobacco Necrosis Virus-1 make sequence-specific contacts t…
View article: Genome-regulated Assembly of a ssRNA Virus May Also Prepare It for Infection
Genome-regulated Assembly of a ssRNA Virus May Also Prepare It for Infection Open
Many single-stranded, positive-sense RNA viruses regulate assembly of their infectious virions by forming multiple, cognate coat protein (CP)-genome contacts at sites termed Packaging Signals (PSs). We have determined the secondary structu…
View article: RNA X-ray footprinting reveals the consequences of an<i>in vivo</i>acquired determinant of viral infectivity
RNA X-ray footprinting reveals the consequences of an<i>in vivo</i>acquired determinant of viral infectivity Open
The secondary structures of the bacteriophage MS2 ssRNA genome, frozen in defined states, were determined with minimal perturbation using constraints from X-ray synchrotron footprinting (XRF). The footprints of the gRNA in the virion and a…
View article: Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts
Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts Open
Picornaviruses are important viral pathogens, but despite extensive study, the assembly process of their infectious virions is still incompletely understood, preventing the development of anti-viral strategies targeting this essential part…
View article: Structural characterization of genomic RNA-coat protein contacts in single-stranded RNA viruses by high-resolution cryo-EM
Structural characterization of genomic RNA-coat protein contacts in single-stranded RNA viruses by high-resolution cryo-EM Open
Recent developments in cryo-electron microscopy (cryo-EM) hardware along with continuously evolving software tools have led to the discovery of many novel structures that it was not possible to solve until now, resulting in what is termed …
View article: Viral Genome Conformations and Contacts across Different Lifecycle Stages
Viral Genome Conformations and Contacts across Different Lifecycle Stages Open
Single-stranded RNA viral genomes (gRNA) are dynamic molecules that permit packaging into virions and their subsequent extrusion during infection. For viruses with such genomes, we discovered a previously unsuspected mechanism that regulat…
View article: Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid
Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid Open
Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on the interior of infectious MS2 virions overlaps with the known peptides that bind the high affinity translational operator, TR, within the phag…