Rebecca E. Colman
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View article: Targeted next-generation sequencing for drug-resistant tuberculosis diagnosis: implementation considerations for bacterial load, regimen selection and diagnostic algorithm placement
Targeted next-generation sequencing for drug-resistant tuberculosis diagnosis: implementation considerations for bacterial load, regimen selection and diagnostic algorithm placement Open
Introduction Early and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is essential for improving treatment outcomes. Phenotypic drug susceptibility testing (pDST) is comprehensive but slow, while rapid molecular assays provide r…
View article: Cost-effectiveness of Targeted Next-Generation Sequencing for Tuberculosis Drug-resistance Testing as an Alternative to the Standard of Care in South Africa
Cost-effectiveness of Targeted Next-Generation Sequencing for Tuberculosis Drug-resistance Testing as an Alternative to the Standard of Care in South Africa Open
Background South Africa faces emerging resistance to tuberculosis drugs like bedaquiline. Phenotypic drug-susceptibility testing (DST), the current reference standard for bedaquiline DST, has long turnaround times. Targeted next-generation…
View article: Comparative assessment of line probe assays and targeted next-generation sequencing in drug-resistant tuberculosis diagnosis
Comparative assessment of line probe assays and targeted next-generation sequencing in drug-resistant tuberculosis diagnosis Open
View article: Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples Open
Next-generation sequencing (NGS) is now recognized as a powerful tool for timely and accurate drug-resistant tuberculosis (DR-TB) diagnosis. Targeted NGS (tNGS) offers a streamlined approach by focusing on specific genes associated with dr…
View article: Facilitating User Interaction with the Tuberculosis Mutation Catalogue using AI Tools
Facilitating User Interaction with the Tuberculosis Mutation Catalogue using AI Tools Open
The World Health Organization (WHO) 2023 Mutation Catalogue for Mycobacterium tuberculosis is a crucial knowledgebase and tool for clinical interpretation of mutations associated with drug-resistant TB. However, the document’s complexity a…
View article: Cost-effectiveness of Targeted Next Generation Sequencing for TB drug-resistance testing as an alternative to the standard of care in South Africa
Cost-effectiveness of Targeted Next Generation Sequencing for TB drug-resistance testing as an alternative to the standard of care in South Africa Open
Background South Africa faces emerging resistance to key TB drugs, including bedaquiline. Phenotypic drug susceptibility testing (pDST), the current reference standard for bedaquiline DST, while accurate has long turnaround times. Targeted…
View article: Evaluating culture-free targeted next-generation sequencing for diagnosing drug-resistant tuberculosis: a multicentre clinical study of two end-to-end commercial workflows
Evaluating culture-free targeted next-generation sequencing for diagnosing drug-resistant tuberculosis: a multicentre clinical study of two end-to-end commercial workflows Open
Unitaid.
View article: Cartridge-based sequencing for bedaquiline resistance detection from sputum
Cartridge-based sequencing for bedaquiline resistance detection from sputum Open
View article: Clinical Evaluation of the XDR-LFC Assay for the Molecular Detection of Isoniazid, Rifampin, Fluoroquinolone, Kanamycin, Capreomycin, and Amikacin Drug Resistance in a Prospective Cohort
Clinical Evaluation of the XDR-LFC Assay for the Molecular Detection of Isoniazid, Rifampin, Fluoroquinolone, Kanamycin, Capreomycin, and Amikacin Drug Resistance in a Prospective Cohort Open
While the goal of universal drug susceptibility testing has been a key component of the WHO End TB Strategy, in practice, this remains inaccessible to many. Rapid molecular tests for tuberculosis (TB) and antituberculosis drug resistance c…
View article: Designing molecular diagnostics for current tuberculosis drug regimens
Designing molecular diagnostics for current tuberculosis drug regimens Open
Diagnostic development must occur in parallel with drug development to ensure the longevity of new treatment compounds. Despite an increasing number of novel and repurposed anti-tuberculosis compounds and regimens, there remains a large nu…
View article: Designing molecular diagnostics for current tuberculosis drug regimens
Designing molecular diagnostics for current tuberculosis drug regimens Open
Diagnostic development must occur in parallel with drug development to ensure the longevity of new treatment compounds. Despite an increasing number of novel and repurposed anti-tuberculosis compounds and regimens, there remains a large nu…
View article: In silico evaluation of WHO-endorsed molecular methods to detect drug resistant tuberculosis
In silico evaluation of WHO-endorsed molecular methods to detect drug resistant tuberculosis Open
View article: Contributions of GeneXpert<sup>®</sup> to TB diagnosis in Myanmar
Contributions of GeneXpert<sup>®</sup> to TB diagnosis in Myanmar Open
BACKGROUND: Xpert ® MTB/RIF, a rapid, molecular TB diagnostic assay, can detect Mycobacterium tuberculosis and rifampin resistance directly from clinical sputum samples in <2 h with high sensitivity and specificity. The added diagnostic va…
View article: In silico evaluation of WHO-endorsed molecular methods to detect drug resistant tuberculosis
In silico evaluation of WHO-endorsed molecular methods to detect drug resistant tuberculosis Open
Universal drug susceptibility testing (DST) for tuberculosis is a major goal of the END TB strategy. PCR-based molecular diagnostic tests have been instrumental in increasing DST globally and several assays have now been endorsed by the Wo…
View article: The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis
The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis Open
Background: Molecular diagnostics are considered the most promising route to achievement of rapid, universal drug susceptibility testing for Mycobacterium tuberculosis complex (MTBC). We aimed to generate a WHO-endorsed catalogue of mutati…
View article: Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system
Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system Open
View article: Invited letter: Integrated palliative care in a geriatric mental health setting during the COVID‐19 pandemic
Invited letter: Integrated palliative care in a geriatric mental health setting during the COVID‐19 pandemic Open
The COVID-19 pandemic has disproportionately impacted the elderly residents of congregate settings with respect to morbidity and mortality. The high rate of severe illness in this population necessitates timely and quality access to pallia…
View article: Age and sex distribution of Mycobacterium tuberculosis infection and rifampicin resistance in Myanmar as detected by Xpert MTB/RIF
Age and sex distribution of Mycobacterium tuberculosis infection and rifampicin resistance in Myanmar as detected by Xpert MTB/RIF Open
Background Detection of tuberculosis disease (TB) and timely identification of Mycobacterium tuberculosis ( Mtb ) strains that are resistant to treatment are key to halting tuberculosis transmission, improving treatment outcomes, and reduc…
View article: Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance
Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance Open
View article: Rapid Detection of Extensively Drug-Resistant Tuberculosis in Clinical Samples Using a Novel Tabletop Platform: Protocol for a Prospective Clinical Study
Rapid Detection of Extensively Drug-Resistant Tuberculosis in Clinical Samples Using a Novel Tabletop Platform: Protocol for a Prospective Clinical Study Open
Background The lack of accurate and efficient diagnostic devices for extensively drug-resistant tuberculosis (XDR-TB) makes it a severe threat to global public health. A prospective clinical study in an intended-use cohort was designed to …
View article: No evidence for enzootic plague within black‐tailed prairie dog (<i>Cynomys ludovicianus</i>) populations
No evidence for enzootic plague within black‐tailed prairie dog (<i>Cynomys ludovicianus</i>) populations Open
Yersinia pestis , causative agent of plague, occurs throughout the western United States in rodent populations and periodically causes epizootics in susceptible species, including black‐tailed prairie dogs ( Cynomys ludovicianus ). How Y. …
View article: The 2021 WHO Catalogue of <i>Mycobacterium Tuberculosis</i> Complex Mutations Associated with Drug Resistance: A New Global Standard for Molecular Diagnostics
The 2021 WHO Catalogue of <i>Mycobacterium Tuberculosis</i> Complex Mutations Associated with Drug Resistance: A New Global Standard for Molecular Diagnostics Open
View article: Rapid Detection of Extensively Drug-Resistant Tuberculosis in Clinical Samples Using a Novel Tabletop Platform: Protocol for a Prospective Clinical Study (Preprint)
Rapid Detection of Extensively Drug-Resistant Tuberculosis in Clinical Samples Using a Novel Tabletop Platform: Protocol for a Prospective Clinical Study (Preprint) Open
BACKGROUND The lack of accurate and efficient diagnostic devices for extensively drug-resistant tuberculosis (XDR-TB) makes it a severe threat to global public health. A prospective clinical study in an intended-use cohort was designed to…
View article: Laboratory Evaluation of a Lateral-Flow Cell for Molecular Detection of First-Line and Second-Line Antituberculosis Drug Resistance
Laboratory Evaluation of a Lateral-Flow Cell for Molecular Detection of First-Line and Second-Line Antituberculosis Drug Resistance Open
Despite the WHO’s call for universal drug susceptibility testing for all patients being evaluated for tuberculosis (TB), a lack of rapid diagnostic tests which can fully describe TB resistance patterns is a major challenge in ensuring that…
View article: Application of Targeted Next-Generation Sequencing Assay on a Portable Sequencing Platform for Culture-Free Detection of Drug-Resistant Tuberculosis from Clinical Samples
Application of Targeted Next-Generation Sequencing Assay on a Portable Sequencing Platform for Culture-Free Detection of Drug-Resistant Tuberculosis from Clinical Samples Open
Targeted next-generation sequencing (tNGS) has emerged as a comprehensive alternative to existing methods for drug susceptibility testing (DST) of Mycobacterium tuberculosis from patient sputum samples for clinical diagnosis of drug-resist…
View article: Review of automated DNA extraction systems for sequencing-based solutions for drug-resistant tuberculosis detection
Review of automated DNA extraction systems for sequencing-based solutions for drug-resistant tuberculosis detection Open
Robust clinical specimen nucleic acid extraction instrumentation and methods are critical to the performance of downstream molecular diagnostics for the diagnosis of drug-resistant tuberculosis (DR-TB). Currently, there is a high level of …
View article: Low cost, low tech SNP genotyping tools for resource-limited areas: Plague in Madagascar as a model
Low cost, low tech SNP genotyping tools for resource-limited areas: Plague in Madagascar as a model Open
Genetic analysis of pathogenic organisms is a useful tool for linking human cases together and/or to potential environmental sources. The resulting data can also provide information on evolutionary patterns within a targeted species and ph…
View article: Lung Transplant Pulmonologists' Views of Specialty Palliative Care for Lung Transplant Recipients
Lung Transplant Pulmonologists' Views of Specialty Palliative Care for Lung Transplant Recipients Open
Background: Lung transplant recipients with serious illness may benefit from but rarely receive specialty palliative care (SPC) services. Transplant pulmonologists' views of SPC may be key to understanding SPC utilization but…
View article: Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation
Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation Open
The iSeq100 instrument is capable of running existing TB WGS and targeted NGS library preparations with comparable accuracy to the MiSeq. The iSeq100 has reduced sequencing workflow hands-on time and is able to deliver sequencing results i…
View article: Guidance for Studies Evaluating the Accuracy of Rapid Tuberculosis Drug-Susceptibility Tests
Guidance for Studies Evaluating the Accuracy of Rapid Tuberculosis Drug-Susceptibility Tests Open
The development and implementation of rapid molecular diagnostics for tuberculosis (TB) drug-susceptibility testing is critical to inform treatment of patients and to prevent the emergence and spread of resistance. Optimal trial planning f…