René Buchet
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View article: Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis
Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis Open
Links between cathepsin K and the pathophysiology of osteoarthritis (OA) can be established, not least because of the overabundance of cathepsin K in the serum of OA patients and the upregulation of cathepsin K in degraded cartilage in ani…
View article: Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?
Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles? Open
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extrac…
View article: The surface proteomic profile of serum extracellular vesicles as a diagnostic and prognostic tool in breast cancer
The surface proteomic profile of serum extracellular vesicles as a diagnostic and prognostic tool in breast cancer Open
The diagnosis of breast cancer in the early stage is essential for a favorable prognosis. Extracellular vesicles isolated from body fluids have a central role in breast cancer development due to their biochemical components. Among the bioc…
View article: Shedding Light on the Role of Na,K-ATPase as a Phosphatase during Matrix-Vesicle-Mediated Mineralization
Shedding Light on the Role of Na,K-ATPase as a Phosphatase during Matrix-Vesicle-Mediated Mineralization Open
Matrix vesicles (MVs) contain the whole machinery necessary to initiate apatite formation in their lumen. We suspected that, in addition to tissue-nonspecific alkaline phosphatase (TNAP), Na,K,-ATPase (NKA) could be involved in supplying p…
View article: Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization
Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization Open
The biochemical machinery involved in matrix vesicles-mediated bone mineralization involves a specific set of lipids, enzymes, and proteins. Annexins, among their many functions, have been described as responsible for the formation and sta…
View article: Inhibition of alkaline phosphatase impairs dyslipidemia and protects mice from atherosclerosis
Inhibition of alkaline phosphatase impairs dyslipidemia and protects mice from atherosclerosis Open
View article: Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation
Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation Open
Matrix vesicles (MVs) are 100–300 nm spherical structures released by mineralization competent cells to initiate formation of apatite, the mineral component in bones. Among proteins present in MVs, annexin A6 (AnxA6) is thought to be ubiqu…
View article: Synthesis of Phospholipids Under Plausible Prebiotic Conditions and Analogies with Phospholipid Biochemistry for Origin of Life Studies
Synthesis of Phospholipids Under Plausible Prebiotic Conditions and Analogies with Phospholipid Biochemistry for Origin of Life Studies Open
Phospholipids are essential components of biological membranes and are involved in cell signalization, in several enzymatic reactions, and in energy metabolism. In addition, phospholipids represent an evolutionary and non-negligible step i…
View article: The functional role of soluble proteins acquired by extracellular vesicles
The functional role of soluble proteins acquired by extracellular vesicles Open
Extracellular vesicles (EVs) are lipid bilayer‐enclosed nanosized particles released by all cell types during physiological as well as pathophysiological processes to carry out diverse biological functions, including acting as sources of c…
View article: Annexins A2, A6 and Fetuin-A Affect the Process of Mineralization in Vesicles Derived from Human Osteoblastic hFOB 1.19 and Osteosarcoma Saos-2 Cells
Annexins A2, A6 and Fetuin-A Affect the Process of Mineralization in Vesicles Derived from Human Osteoblastic hFOB 1.19 and Osteosarcoma Saos-2 Cells Open
The mineralization process is initiated by osteoblasts and chondrocytes during intramembranous and endochondral ossifications, respectively. Both types of cells release matrix vesicles (MVs), which accumulate Pi and Ca2+ and form apatites …
View article: Hydrolysis of Extracellular ATP by Vascular Smooth Muscle Cells Transdifferentiated into Chondrocytes Generates Pi but Not PPi
Hydrolysis of Extracellular ATP by Vascular Smooth Muscle Cells Transdifferentiated into Chondrocytes Generates Pi but Not PPi Open
(1) Background: Tissue non-specific alkaline phosphatase (TNAP) is suspected to induce atherosclerosis plaque calcification. TNAP, during physiological mineralization, hydrolyzes the mineralization inhibitor inorganic pyrophosphate (PPi). …
View article: Peer Review #3 of "Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes (v0.1)"
Peer Review #3 of "Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes (v0.1)" Open
Background: In this study, we investigated the effect of the mechanical loading history on the expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) in MLO-Y4 osteocyte-like cells.Methods: Thre…
View article: Revealing the localization of Annexin A6 in matrix vesicles during physiological mineralization
Revealing the localization of Annexin A6 in matrix vesicles during physiological mineralization Open
View article: Sphingosine 1-phosphate is a likely important factor exacerbating vascular calcification in chronic kidney disease
Sphingosine 1-phosphate is a likely important factor exacerbating vascular calcification in chronic kidney disease Open
View article: Unravelling the role of phosphatidylserine on the precipitation of calcium phosphate by mineralizing-extracellular vesicles
Unravelling the role of phosphatidylserine on the precipitation of calcium phosphate by mineralizing-extracellular vesicles Open
View article: Localization of Annexin A6 and its translocation across membrane in matrix vesicles during apatite formation
Localization of Annexin A6 and its translocation across membrane in matrix vesicles during apatite formation Open
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View article: Symmetry Breaking of Phospholipids
Symmetry Breaking of Phospholipids Open
Either stereo reactants or stereo catalysis from achiral or chiral molecules are a prerequisite to obtain pure enantiomeric lipid derivatives. We reviewed a few plausibly organic syntheses of phospholipids under prebiotic conditions with s…
View article: Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization
Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization Open
View article: Prostate cancer-derived exosomes promote osteoblast differentiation and activity through phospholipase D2
Prostate cancer-derived exosomes promote osteoblast differentiation and activity through phospholipase D2 Open
View article: Symmetry Breaking of Phospholipids
Symmetry Breaking of Phospholipids Open
Either stereo reactants or stereo catalysis from achiral or chiral molecules are prerequisite to obtain pure enantiomeric lipid derivatives. We reviewed a few plausible organic syntheses of phospholipids under prebiotic conditions with a s…
View article: Racemic Phospholipids for Origin of Life Studies
Racemic Phospholipids for Origin of Life Studies Open
Although prebiotic condensations of glycerol, phosphate and fatty acids produce phospholipid esters with a racemic backbone, most experimental studies on vesicles intended as protocell models have been carried out by employing commercial e…
View article: Localization of Annexin A6 in Matrix Vesicles During Physiological Mineralization
Localization of Annexin A6 in Matrix Vesicles During Physiological Mineralization Open
Annexin A6 (AnxA6) is the largest member of the annexin family of proteins present in matrix vesicles (MVs). MVs are a special class of extracellular vesicles that serve as a nucleation site during cartilage, bone, and mantle dentin minera…
View article: Tissue-nonspecific alkaline phosphatase is an anti-inflammatory nucleotidase
Tissue-nonspecific alkaline phosphatase is an anti-inflammatory nucleotidase Open
View article: Src and ROCK Kinases Differentially Regulate Mineralization of Human Osteosarcoma Saos-2 Cells
Src and ROCK Kinases Differentially Regulate Mineralization of Human Osteosarcoma Saos-2 Cells Open
Osteoblasts initiate bone mineralization by releasing matrix vesicles (MVs) into the extracellular matrix (ECM). MVs promote the nucleation process of apatite formation from Ca2+ and Pi in their lumen and bud from the microvilli of osteobl…
View article: Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization
Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization Open
View article: Design, synthesis and biological evaluation of inhibitors of cathepsin K on dedifferentiated chondrocytes
Design, synthesis and biological evaluation of inhibitors of cathepsin K on dedifferentiated chondrocytes Open
View article: Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis Open
This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. These cell lines…
View article: Crude phosphorylation mixtures containing racemic lipid amphiphiles self-assemble to give stable primitive compartments
Crude phosphorylation mixtures containing racemic lipid amphiphiles self-assemble to give stable primitive compartments Open
View article: Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models
Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models Open
View article: Correction: Giant vesicles from rehydrated crude mixtures containing unexpected mixtures of amphiphiles formed under plausibly prebiotic conditions
Correction: Giant vesicles from rehydrated crude mixtures containing unexpected mixtures of amphiphiles formed under plausibly prebiotic conditions Open
Correction for ‘Giant vesicles from rehydrated crude mixtures containing unexpected mixtures of amphiphiles formed under plausibly prebiotic conditions’ by Michele Fiore et al., Org. Biomol. Chem., 2017, 15, 4231–4240.