René Buschow
YOU?
Author Swipe
View article: Differential oligomerization regulates PHF13 chromatin affinity and function
Differential oligomerization regulates PHF13 chromatin affinity and function Open
PHF13 is a H3K4me3 epigenetic reader that modulates key chromatin processes including transcription, DNA damage response, and chromatin architecture. PHF13 is found aberrantly regulated in different cancers and its misexpression alters the…
View article: Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes
Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes Open
Mesenchymal stem cells (MSC) are fibroblast-like non-hematopoietic cells with self-renewal and differentiation capacity, and thereby great potential in regeneration and wound healing. MSC populations are heterogeneous not only inherently, …
View article: An activity-specificity trade-off encoded in human transcription factors
An activity-specificity trade-off encoded in human transcription factors Open
Transcription factors (TFs) control specificity and activity of gene transcription, but whether a relationship between these two features exists is unclear. Here we provide evidence for an evolutionary trade-off between the activity and sp…
View article: Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids
Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids Open
Embryogenesis requires substantial coordination to translate genetic programs to the collective behavior of differentiating cells, but understanding how cellular decisions control tissue morphology remains conceptually and technically chal…
View article: GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers
GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers Open
X-chromosome inactivation (XCI) balances gene expression between the sexes in female mammals. Shortly after fertilization, upregulation of Xist RNA from one X chromosome initiates XCI, leading to chromosome-wide gene silencing. XCI is main…
View article: Movie S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Movie S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Live-cell fluorescence imaging showed that the DQ-Red BSA accumulated in UOK276 cells over time, providing evidence that macropinocytosis is an established process in chRCC cells to uptake macromolecules as nutrient source.
View article: Table S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Table S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Proteome profiling and a subsequent GSEA analysis of UOK276 cells supplemented with 1% amino acids in the presence or absence of 2.5% BSA versus a complete medium.
View article: Supplementary figures from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Supplementary figures from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Supplementary figures and figure legends
View article: Table S2 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Table S2 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Somatic nonsynonymous SNV and indels identified in our corhort.
View article: Table S2 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Table S2 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Somatic nonsynonymous SNV and indels identified in our corhort.
View article: Table S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Table S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Proteome profiling and a subsequent GSEA analysis of UOK276 cells supplemented with 1% amino acids in the presence or absence of 2.5% BSA versus a complete medium.
View article: Supplementary figures from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Supplementary figures from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Supplementary figures and figure legends
View article: Captions of supplementary movie and tables from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Captions of supplementary movie and tables from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Captions of supplementary movie and tables
View article: Captions of supplementary movie and tables from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Captions of supplementary movie and tables from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Captions of supplementary movie and tables
View article: Data from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Data from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Chromophobe renal cell carcinoma (chRCC) accounts for approximately 5% of all renal cancers and around 30% of chRCC cases have mutations in TP53. chRCC is poorly supported by microvessels and has markably lower glucose uptake than c…
View article: Data from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Data from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Chromophobe renal cell carcinoma (chRCC) accounts for approximately 5% of all renal cancers and around 30% of chRCC cases have mutations in TP53. chRCC is poorly supported by microvessels and has markably lower glucose uptake than c…
View article: Movie S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma
Movie S1 from Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma Open
Live-cell fluorescence imaging showed that the DQ-Red BSA accumulated in UOK276 cells over time, providing evidence that macropinocytosis is an established process in chRCC cells to uptake macromolecules as nutrient source.
View article: Aberrant phase separation and nucleolar dysfunction in rare genetic diseases
Aberrant phase separation and nucleolar dysfunction in rare genetic diseases Open
View article: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation Open
Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. Despite much progress over the past decade, the genome-wide annotation of …
View article: Author response: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Author response: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation Open
View article: Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids
Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids Open
The cellular microenvironment, together with intrinsic regulators, shapes stem cell identity and differentiation capacity. Mammalian early embryos are exposed to hypoxia in vivo and appear to benefit from hypoxic culture in vitro. Yet, how…
View article: Discovery and characterization of <i>LNCSOX17</i> as an essential regulator in human endoderm formation
Discovery and characterization of <i>LNCSOX17</i> as an essential regulator in human endoderm formation Open
Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. Despite much progress over the past decade, the genome-wide annotation of …
View article: GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of a distal enhancer element
GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of a distal enhancer element Open
To ensure dosage compensation for X-linked genes between the sexes, one X chromosome is silenced during early embryonic development of female mammals. This process of X-chromosome inactivation (XCI) is initiated through upregulation of the…
View article: Hijacking of transcriptional condensates by endogenous retroviruses
Hijacking of transcriptional condensates by endogenous retroviruses Open
View article: Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells
Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells Open
Cerebral organoids exhibit broad regional heterogeneity accompanied by limited cortical cellular diversity despite the tremendous upsurge in derivation methods, suggesting inadequate patterning of early neural stem cells (NSCs). Here we sh…
View article: Connecting the Dots: PHF13 and cohesin promote polymer-polymer phase separation of chromatin into chromosomes
Connecting the Dots: PHF13 and cohesin promote polymer-polymer phase separation of chromatin into chromosomes Open
Summary How interphase chromatin compacts into mitotic chromosomes has eluded researchers for over a century. Here we show that PHF13, a tightly regulated H3K4me epigenetic reader, and cohesin, a mediator of chromatin architecture, coopera…
View article: Connecting the Dots: PHF13 and Cohesin Promote Polymer-Polymer Phase Separation of Chromatin Into Chromosomes
Connecting the Dots: PHF13 and Cohesin Promote Polymer-Polymer Phase Separation of Chromatin Into Chromosomes Open
View article: Hijacking of transcriptional condensates by endogenous retroviruses
Hijacking of transcriptional condensates by endogenous retroviruses Open
Endogenous retroviruses (ERVs) comprise ~10% of mammalian genomes, and ERVs are repressed by multiple cellular mechanisms including heterochromatin, DNA methylation, and methylation of their RNA transcripts 1-13. Although the vast majority…
View article: Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changes
Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changes Open
Glucocorticoids are stress hormones that elicit cellular responses by binding to the glucocorticoid receptor, a ligand-activated transcription factor. The exposure of cells to this hormone induces wide-spread changes in the chromatin lands…
View article: Hypoxia induces a transcriptional early primitive streak signature in pluripotent cells enhancing spontaneous elongation and lineage representation in gastruloids
Hypoxia induces a transcriptional early primitive streak signature in pluripotent cells enhancing spontaneous elongation and lineage representation in gastruloids Open
The cellular microenvironment together with intrinsic regulators shapes stem cell identity and differentiation capacity. Mammalian early embryos are exposed to hypoxia in vivo and appear to benefit from hypoxic culture in vitro. Yet, compo…