René M. de Jong
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View article: Structural and time‐resolved mechanistic investigations of protein hydrolysis by the acidic proline‐specific endoprotease from <i>Aspergillus niger</i>
Structural and time‐resolved mechanistic investigations of protein hydrolysis by the acidic proline‐specific endoprotease from <i>Aspergillus niger</i> Open
Proline‐specific endoproteases have been successfully used in, for example, the in‐situ degradation of gluten, the hydrolysis of bitter peptides, the reduction of haze during beer production, and the generation of peptides for mass spectro…
View article: Computation-Aided Engineering of Cytochrome P450 for the Production of Pravastatin
Computation-Aided Engineering of Cytochrome P450 for the Production of Pravastatin Open
CYP105AS1 is a cytochrome P450 from Amycolatopsis orientalis that catalyzes monooxygenation of compactin to 6-epi-pravastatin. For fermentative production of the cholesterol-lowering drug pravastatin, the stereoselectivity of the en…
View article: Introducing an Artificial Deazaflavin Cofactor in <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae</i>
Introducing an Artificial Deazaflavin Cofactor in <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae</i> Open
Deazaflavin-dependent whole-cell conversions in well-studied and industrially relevant microorganisms such as Escherichia coli and Saccharomyces cerevisiae have high potential for the biocatalytic production of valuable compo…
View article: Macromolecular modeling and design in Rosetta: recent methods and frameworks
Macromolecular modeling and design in Rosetta: recent methods and frameworks Open
View article: Rhodoxanthin synthase from honeysuckle; a membrane diiron enzyme catalyzes the multistep conversion of β-carotene to rhodoxanthin
Rhodoxanthin synthase from honeysuckle; a membrane diiron enzyme catalyzes the multistep conversion of β-carotene to rhodoxanthin Open
A novel β-carotene hydroxylase catalyzes the formation of rhodoxanthin, an unusual carotenoid in red honeysuckle berries.
View article: Metal Dependence of the Xylose Isomerase from<i>Piromyces</i>sp. E2 Explored by Activity Profiling and Protein Crystallography
Metal Dependence of the Xylose Isomerase from<i>Piromyces</i>sp. E2 Explored by Activity Profiling and Protein Crystallography Open
Xylose isomerase from Piromyces sp. E2 (PirXI) can be used to equip Saccharomyces cerevisiae with the capacity to ferment xylose to ethanol. The biochemical properties and structure of the enzyme have not been described even though its met…
View article: CorNet: Assigning function to networks of co-evolving residues by automated literature mining
CorNet: Assigning function to networks of co-evolving residues by automated literature mining Open
CorNet is a web-based tool for the analysis of co-evolving residue positions in protein super-family sequence alignments. CorNet projects external information such as mutation data extracted from literature on interactively displayed group…
View article: An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae
An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae Open
View article: Coupling Protein Side-Chain and Backbone Flexibility Improves the Re-design of Protein-Ligand Specificity
Coupling Protein Side-Chain and Backbone Flexibility Improves the Re-design of Protein-Ligand Specificity Open
Interactions between small molecules and proteins play critical roles in regulating and facilitating diverse biological functions, yet our ability to accurately re-engineer the specificity of these interactions using computational approach…