René Uebe
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View article: Engineering NIR-sighted bacteria
Engineering NIR-sighted bacteria Open
Spatially and temporally orchestrated gene expression underpins organismal development, physiology, and adaptation. In bacteria, two-component systems (TCS) translate environmental cues into inducible expression outputs. Inducible expressi…
View article: Engineering NIR-Sighted Bacteria
Engineering NIR-Sighted Bacteria Open
Spatially and temporally orchestrated gene expression underpins organismal development, physiology, and adaptation. In bacteria, two-component systems (TCS) translate environmental cues into inducible expression outputs. Inducible expressi…
View article: Engineering NIR-Sighted Bacteria
Engineering NIR-Sighted Bacteria Open
Spatially and temporally orchestrated gene expression underpins organismal development, physiology, and adaptation. In bacteria, two-component systems (TCS) translate environmental cues into inducible expression outputs. Inducible expressi…
View article: Engineering NIR-sighted bacteria
Engineering NIR-sighted bacteria Open
Spatially and temporally orchestrated gene expression underpins organismal development, physiology, and adaptation. In bacteria, two-component systems (TCS) translate environmental cues into inducible expression outputs. Inducible expressi…
View article: Biogenese magnetischer Organellen in Bakterien
Biogenese magnetischer Organellen in Bakterien Open
For geomagnetic navigation, magnetotactic bacteria (MTB) form magnetosomes. Biogenesis of these unique magnetic organelles is a stepwise process controlled by more than 30 magnetosome-associated proteins. Recent findings suggest that magne…
View article: Engineering NIR-Sighted Bacteria
Engineering NIR-Sighted Bacteria Open
Spatially and temporally orchestrated gene expression underpins organismal development, physiology, and adaptation. In bacteria, two-component systems (TCS) translate environmental cues into inducible expression outputs. Inducible expressi…
View article: Homologs of the plastidal preprotein translocase Tic20 mediate organelle assembly in bacteria
Homologs of the plastidal preprotein translocase Tic20 mediate organelle assembly in bacteria Open
Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we identified that MamF-like proteins involved in the formati…
View article: Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium
Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium Open
Horizontal gene transfer is a powerful source of innovations in prokaryotes that can affect almost any cellular system, including microbial organelles. The formation of magnetosomes, one of the most sophisticated microbial mineral-containi…
View article: Hidden Talents: Silent Gene Clusters Encoding Magnetic Organelle Biosynthesis in a Non-Magnetotactic Phototrophic Bacterium
Hidden Talents: Silent Gene Clusters Encoding Magnetic Organelle Biosynthesis in a Non-Magnetotactic Phototrophic Bacterium Open
Magnetosomes are unique organelles synthesized by magnetotactic bacteria (MTB) for magnetic navigation. Their complex biosynthesis is controlled by large magnetosome gene clusters (MGC). Here, we report the discovery and comprehensive anal…
View article: Synchrotron‐Based Nano‐X‐Ray Absorption Near‐Edge Structure Revealing Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria
Synchrotron‐Based Nano‐X‐Ray Absorption Near‐Edge Structure Revealing Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria Open
Magnetotactic bacteria (MTB) sequester iron from the environment to biomineralize magnetite or greigite nanoparticles in magnetosome organelles, though the necessity of intracellular iron storage for the formation process is still in quest…
View article: The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense
The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense Open
Magnetosomes have emerged as a model system to study prokaryotic organelles and a source of biocompatible magnetic nanoparticles for various biomedical applications. However, the lack of knowledge about the transcriptional organization of …
View article: Synchrotron-Based Nano-XANES Reveals Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria
Synchrotron-Based Nano-XANES Reveals Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria Open
This report demonstrates how scanning X-ray fluorescence microscopy (SXFM) and nanoscale X-ray absorption near-edge structure (nano-XANES) can spatially and chemically identify intracellular iron species at the single-cell level, creating …
View article: Synchrotron-Based Nano-XANES Reveals Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria
Synchrotron-Based Nano-XANES Reveals Intracellular Heterogeneity of Iron Species in Magnetotactic Bacteria Open
This report demonstrates how scanning X-ray fluorescence microscopy (SXFM) and nanoscale X-ray absorption near-edge structure (nano-XANES) can spatially and chemically identify intracellular iron species at the single-cell level, creating …
View article: Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense
Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense Open
Background Magnetosome formation in the alphaproteobacterium Magnetospirillum gryphiswaldense is controlled by more than 30 known mam and mms genes clustered within a large genomic region, the ‘magnetosome island’ (MAI), which also harbors…
View article: Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense
Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense Open
Magnetospirillum gryphiswaldense is one of the few tractable model magnetotactic bacteria (MTB) for studying magnetosome biomineralization. So far, knowledge on the genetic determinants of this complex process has been mainly gathered usin…
View article: Towards standardized purification of bacterial magnetic nanoparticles for future in vivo applications
Towards standardized purification of bacterial magnetic nanoparticles for future in vivo applications Open
Bacterial magnetosomes (MS) are well-defined membrane-enveloped single-domain iron oxide (magnetite) nanoparticles, which are susceptible to genetic and chemical engineering. Additionally, the possibility to manipulate these particles by e…
View article: Single‐step transfer of biosynthetic operons endows a non‐magnetotactic <i>Magnetospirillum</i> strain from wetland with magnetosome biosynthesis
Single‐step transfer of biosynthetic operons endows a non‐magnetotactic <i>Magnetospirillum</i> strain from wetland with magnetosome biosynthesis Open
Summary The magnetotactic lifestyle represents one of the most complex traits found in many bacteria from aquatic environments and depends on magnetic organelles, the magnetosomes. Genetic transfer of magnetosome biosynthesis operons to a …
View article: Bacterioferritin of Magnetospirillum gryphiswaldense Is a Heterotetraeicosameric Complex Composed of Functionally Distinct Subunits but Is Not Involved in Magnetite Biomineralization
Bacterioferritin of Magnetospirillum gryphiswaldense Is a Heterotetraeicosameric Complex Composed of Functionally Distinct Subunits but Is Not Involved in Magnetite Biomineralization Open
Magnetotactic bacteria like Magnetospirillum gryphiswaldense are able to orient along magnetic field lines due to the intracellular formation of magnetite nanoparticles. Biomineralization of magnetite has been suggested to require a yet-un…
View article: Numerical unmixing of weakly and strongly magnetic minerals: examples with synthetic mixtures of magnetite and hematite
Numerical unmixing of weakly and strongly magnetic minerals: examples with synthetic mixtures of magnetite and hematite Open
ISSN:0956-540X
View article: Reevaluation of the Complete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1 with Single-Molecule Real-Time Sequencing Data
Reevaluation of the Complete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1 with Single-Molecule Real-Time Sequencing Data Open
Magnetospirillum gryphiswaldense is a key organism for understanding magnetosome formation and magnetotaxis. As earlier studies suggested a high genomic plasticity, we (re)sequenced the type strain MSR-1 and the laboratory strain R3/S1. Bo…
View article: MPS and MRI efficacy of magnetosomes from wild-type and mutant bacterial strains
MPS and MRI efficacy of magnetosomes from wild-type and mutant bacterial strains Open
The future of Magnetic Particle Imaging (MPI), as a tracer-based imaging modality, crucially relies on the develop- ment of high-performing tracers. Due to their ideal structural and magnetic properties, biogenic nanoparticles extracted fr…
View article: Genetic and Ultrastructural Analysis Reveals the Key Players and Initial Steps of Bacterial Magnetosome Membrane Biogenesis
Genetic and Ultrastructural Analysis Reveals the Key Players and Initial Steps of Bacterial Magnetosome Membrane Biogenesis Open
Magnetosomes of magnetotactic bacteria contain well-ordered nanocrystals for magnetic navigation and have recently emerged as the most sophisticated model system to study the formation of membrane bounded organelles in prokaryotes. Magneto…
View article: Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium
Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium Open
Magnetotactic bacteria biosynthesize specific organelles, the magnetosomes, which are membrane-enclosed crystals of a magnetic iron mineral that are aligned in a linear chain. The number and size of magnetosome particles have to be critica…