Renske van Raaphorst
YOU?
Author Swipe
View article: Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle Open
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA replication followed by non-binary division …
View article: HU promotes higher order chromosome organization and influences DNA replication rates in <i>Streptococcus pneumoniae</i>
HU promotes higher order chromosome organization and influences DNA replication rates in <i>Streptococcus pneumoniae</i> Open
Nucleoid-associated proteins (NAPs) are crucial for maintaining chromosomal compaction and architecture, and are actively involved in DNA replication, recombination, repair, and gene regulation. In Streptococcus pneumoniae, the role of the…
View article: Future Directions of the Prokaryotic Chromosome Field
Future Directions of the Prokaryotic Chromosome Field Open
In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around curren…
View article: Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions
Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions Open
View article: Rewiring capsule production by CRISPRi-based genetic oscillators demonstrates a functional role of phenotypic variation in pneumococcal-host interactions
Rewiring capsule production by CRISPRi-based genetic oscillators demonstrates a functional role of phenotypic variation in pneumococcal-host interactions Open
Phenotypic variation is the phenomenon in which clonal cells display different traits even under identical environmental conditions. This plasticity is thought to be important for processes including bacterial virulence 1–8 , but direct ev…
View article: Modulation of prey size reveals adaptability and robustness in the cell cycle of an intracellular predator
Modulation of prey size reveals adaptability and robustness in the cell cycle of an intracellular predator Open
View article: Modulation of prey size reveals adaptability and robustness in the cell cycle of an intracellular predator
Modulation of prey size reveals adaptability and robustness in the cell cycle of an intracellular predator Open
Summary Despite the remarkable diversity of bacterial lifestyles, the sophisticated regulatory networks underlying bacterial replication have only been investigated in a limited number of model species so far. In bacteria that do not rely …
View article: CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA
CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA Open
Most bacteria replicate and segregate their DNA concomitantly while growing, before cell division takes place. How bacteria synchronize these different cell cycle events to ensure faithful chromosome inheritance by daughter cells is poorly…
View article: Chromosome choreography during the non-binary cell cycle of a predatory bacterium
Chromosome choreography during the non-binary cell cycle of a predatory bacterium Open
View article: Chromosome Choreography During the Non-Binary Cell Cycle of a Predatory Bacterium
Chromosome Choreography During the Non-Binary Cell Cycle of a Predatory Bacterium Open
View article: Chromosome dynamics and spatial organization during the non-binary cell cycle of a predatory bacterium
Chromosome dynamics and spatial organization during the non-binary cell cycle of a predatory bacterium Open
In bacteria, the dynamics of chromosome replication and segregation are tightly coordinated with cell cycle progression, and largely rely on specific spatiotemporal arrangement of the chromosome. Whereas these key processes are mostly inve…
View article: Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events Open
The spread of antimicrobial resistance and vaccine escape in the human pathogen Streptococcus pneumoniae can be largely attributed to competence-induced transformation. Here, we studied this process at the single-cell level. We show that w…
View article: Author response: Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
Author response: Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events Open
View article: Anatomy of the pneumococcal nucleoid: Visualizing replication, chromosome segregation and chromosome condensation dynamics in Streptococcus pneumoniae
Anatomy of the pneumococcal nucleoid: Visualizing replication, chromosome segregation and chromosome condensation dynamics in Streptococcus pneumoniae Open
The pneumococcus is a bacterium that lives in the upper part of the throat behind the nose of many children and adults. In most cases it lives there harmlessly, but sometimes, it can move further into the body and cause serious illnesses a…
View article: Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events Open
The rapid spread of antimicrobial resistance and vaccine escape in the opportunistic human pathogen Streptococcus pneumoniae can be largely attributed to competence-induced transformation. To better understand the dynamics of competence-in…
View article: BactMAP: An R package for integrating, analyzing and visualizing bacterial microscopy data
BactMAP: An R package for integrating, analyzing and visualizing bacterial microscopy data Open
High‐throughput analyses of single‐cell microscopy data are a critical tool within the field of bacterial cell biology. Several programs have been developed to specifically segment bacterial cells from phase‐contrast images. Together with …
View article: Spatio-temporal control of DNA replication by the pneumococcal cell cycle regulator CcrZ
Spatio-temporal control of DNA replication by the pneumococcal cell cycle regulator CcrZ Open
Most bacteria replicate and segregate their DNA concomitantly while growing, before cell division takes place. How bacteria synchronize these different cell cycle events to ensure faithful chromosome inheritance is poorly understood. Here,…
View article: BactMAP: an R package for integrating, analyzing and visualizing bacterial microscopy data
BactMAP: an R package for integrating, analyzing and visualizing bacterial microscopy data Open
High-throughput analyses of single-cell microscopy data is a critical tool within the field of bacterial cell biology. Several programs have been developed to specifically segment bacterial cells from phase-contrast images. Together with s…
View article: Chromosome segregation drives division site selection in <i>Streptococcus pneumoniae</i>
Chromosome segregation drives division site selection in <i>Streptococcus pneumoniae</i> Open
Significance Precise cell division is crucial for all cellular life. In the pneumococcus, which kills more than a million people annually, the protein MapZ was recently identified as a marker for the division site. By developing a suite of…
View article: Chromosome segregation drives division site selection in<i>Streptococcus pneumoniae</i>
Chromosome segregation drives division site selection in<i>Streptococcus pneumoniae</i> Open
Accurate spatial and temporal positioning of the tubulin-like protein FtsZ is key for proper bacterial cell division. Streptococcus pneumoniae (pneumococcus) is an oval-shaped, symmetrically dividing human pathogen lacking the canonical sy…
View article: Red Fluorescent Proteins for Gene Expression and Protein Localization Studies in Streptococcus pneumoniae and Efficient Transformation with DNA Assembled via the Gibson Assembly Method
Red Fluorescent Proteins for Gene Expression and Protein Localization Studies in Streptococcus pneumoniae and Efficient Transformation with DNA Assembled via the Gibson Assembly Method Open
During the last decades, a wide range of fluorescent proteins (FPs) have been developed and improved. This has had a great impact on the possibilities in biological imaging and the investigation of cellular processes at the single-cell lev…