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View article: AC-YOLO: A lightweight ship detection model for SAR images based on YOLO11
AC-YOLO: A lightweight ship detection model for SAR images based on YOLO11 Open
Synthetic Aperture Radar (SAR), renowned for its all-weather monitoring capability and high-resolution imaging characteristics, plays a pivotal role in ocean resource exploration, environmental surveillance, and maritime security. It has b…
View article: Negative Regulation of GmNAC35 by miR164 Enhances Drought Tolerance in Soybean
Negative Regulation of GmNAC35 by miR164 Enhances Drought Tolerance in Soybean Open
Soybean (Glycine max (L.) Merr.) is a critical crop in China, serving as a primary source of food, oil, and animal feed. Drought stress significantly impacts soybean growth and yield. MicroRNAs (miRNAs) play crucial roles in plant drought …
View article: Protocol for detecting lncRNA-protein interaction in vivo using the yeast three-hybrid assay
Protocol for detecting lncRNA-protein interaction in vivo using the yeast three-hybrid assay Open
Analyses of long non-coding RNA (lncRNA)-protein interactions provide key clues for understanding the molecular basis of lncRNA-modulated biological processes. Here, we detail a yeast three-hybrid assay to identify the lncRNA-interacting p…
View article: Protocol for detecting lncRNA-protein interactions in vitro by tRSA RNA pull-down assay
Protocol for detecting lncRNA-protein interactions in vitro by tRSA RNA pull-down assay Open
Long non-coding RNAs (lncRNAs) work together with diverse RNA-binding proteins (RBPs) to fulfill key regulations in important cellular functions. Here, we present a protocol to detect lncRNA-RBP interactions in vitro using a tRNA scaffold …
View article: LncRNA DANA1 promotes drought tolerance and histone deacetylation of drought responsive genes in Arabidopsis
LncRNA DANA1 promotes drought tolerance and histone deacetylation of drought responsive genes in Arabidopsis Open
Although many long noncoding RNAs have been discovered in plants, little is known about their biological function and mode of action. Here we show that the drought-induced long intergenic noncoding RNA DANA1 interacts with the L1p/L10e fam…
View article: Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system Open
DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic d…
View article: Coordinating Diverse Functions of miRNA and lncRNA in Fleshy Fruit
Coordinating Diverse Functions of miRNA and lncRNA in Fleshy Fruit Open
Non-coding RNAs play vital roles in the diverse biological processes of plants, and they are becoming key topics in horticulture research. In particular, miRNAs and long non-coding RNAs (lncRNAs) are receiving increased attention in fruit …
View article: The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic
The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic Open
Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based …
View article: Mutations in <i><scp>MIR</scp>396e</i> and <i><scp>MIR</scp>396f</i> increase grain size and modulate shoot architecture in rice
Mutations in <i><span>MIR</span>396e</i> and <i><span>MIR</span>396f</i> increase grain size and modulate shoot architecture in rice Open
Summary Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR 396 gene family in rice and found that MIR 396e and MIR 396f are two important regulators of grain siz…
View article: A photo‐responsive F‐box protein <scp>FOF</scp>2 regulates floral initiation by promoting <i><scp>FLC</scp></i> expression in Arabidopsis
A photo‐responsive F‐box protein <span>FOF</span>2 regulates floral initiation by promoting <i><span>FLC</span></i> expression in Arabidopsis Open
Summary Floral initiation is regulated by various genetic pathways in response to light, temperature, hormones and developmental status; however, the molecular mechanisms underlying the interactions between different genetic pathways are n…