Richard A. Willis
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View article: Shortwave-infrared-light-emitting probes for the in vivo tracking of cancer vaccines and the elicited immune responses
Shortwave-infrared-light-emitting probes for the in vivo tracking of cancer vaccines and the elicited immune responses Open
Tracking and imaging immune cells in vivo non-invasively would offer insights into the immune responses induced by vaccination. Here we report a cancer vaccine consisting of polymer-coated NaErF 4 /NaYF 4 core–shell down-conversion nanopar…
View article: Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection
Spheromers reveal robust T cell responses to the Pfizer/BioNTech vaccine and attenuated peripheral CD8+ T cell responses post SARS-CoV-2 infection Open
T cells are a critical component of the response to SARS-CoV-2, but their kinetics after infection and vaccination are insufficiently understood. Using "spheromer" peptide-MHC multimer reagents, we analyzed healthy subjects receiving two d…
View article: Minimal Information about MHC Multimers (MIAMM)
Minimal Information about MHC Multimers (MIAMM) Open
With the goal of improving the reproducibility and annotatability of MHC multimer reagent data, we present the establishment of a new data standard: Minimal Information about MHC Multimers (https://miamm.lji.org/). Multimers are engineered…
View article: CD1a selectively captures endogenous cellular lipids that broadly block T cell response
CD1a selectively captures endogenous cellular lipids that broadly block T cell response Open
We optimized lipidomics methods to broadly detect endogenous lipids bound to cellular CD1a proteins. Whereas membrane phospholipids dominate in cells, CD1a preferentially captured sphingolipids, especially a C42, doubly unsaturated sphingo…
View article: Production of Class II MHC Proteins in Lentiviral Vector‐Transduced HEK‐293T Cells for Tetramer Staining Reagents
Production of Class II MHC Proteins in Lentiviral Vector‐Transduced HEK‐293T Cells for Tetramer Staining Reagents Open
Class II major histocompatibility complex peptide (MHC‐IIp) multimers are precisely engineered reagents used to detect T cells specific for antigens from pathogens, tumors, and self‐proteins. While the related Class I MHC/peptide (MHC‐Ip) …
View article: Human skin is colonized by T cells that recognize CD1a independently of lipid
Human skin is colonized by T cells that recognize CD1a independently of lipid Open
CD1a-autoreactive T cells contribute to skin disease, but the identity of immunodominant self-lipid antigens and their mode of recognition are not yet solved. In most models, MHC and CD1 proteins serve as display platforms for smaller anti…
View article: Synthesis, stabilization, and characterization of the MR1 ligand precursor 5-amino-6-D-ribitylaminouracil (5-A-RU)
Synthesis, stabilization, and characterization of the MR1 ligand precursor 5-amino-6-D-ribitylaminouracil (5-A-RU) Open
Mucosal-associated invariant T (MAIT) cells are an abundant class of innate T cells restricted by the MHC I-related molecule MR1. MAIT cells can recognize bacterially-derived metabolic intermediates from the riboflavin pathway presented by…
View article: A miRNA181a/NFAT5 axis links impaired T cell tolerance induction with autoimmune type 1 diabetes
A miRNA181a/NFAT5 axis links impaired T cell tolerance induction with autoimmune type 1 diabetes Open
A miRNA181a/NFAT5 signaling axis promotes immune activation and interferes with the induction of regulatory T cells in islet autoimmunity.
View article: miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity
miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity Open
Significance The onset of type 1 diabetes autoimmunity is indicated by the development of multiple islet autoantibodies, produced by B cells with the help of T follicular helper (TFH) cells. MicroRNAs (miRNAs) are small noncoding RNAs that…