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View article: Pseudomonas aeruginosa ATCC 49189, a new quality control strain for testing P. aeruginosa susceptibility to the aminoglycosides
Pseudomonas aeruginosa ATCC 49189, a new quality control strain for testing P. aeruginosa susceptibility to the aminoglycosides Open
Quality control for in vitro antimicrobial susceptibility testing of Pseudomonas aeruginosa versus aminoglycosides is improved by P. aeruginosa ATCC 49189, which was developed in the Clinical Microbiology Laboratory at St. Paul-Ramsey Medi…
View article: Macrodilution MBC result reproducibility for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates
Macrodilution MBC result reproducibility for methicillin-susceptible and reputedly tolerant Staphylococcus aureus isolates Open
Macrodilution MBC Result Reproducibility for Methicillin-Susceptible and Reputedly Tolerant Staphylococcus aureus IsolatesWe wish to comment on the paper by Pelletier and Baker that appeared in the March issue of this journal (7).Our Staph…
View article: Penicillin Tolerance in Group A Streptococci
Penicillin Tolerance in Group A Streptococci Open
Journal Article Penicillin Tolerance in Group A Streptococci Get access Bert F. Woolfrey, Bert F. Woolfrey Section of Microbiology, Department of Pathology, St. Paul-Ramsey Medical Center, St. Paul, Minnesota Please address requests for re…
View article: Effect of temperature on inoculum as a potential source of error in agar dilution plate count bactericidal measurements
Effect of temperature on inoculum as a potential source of error in agar dilution plate count bactericidal measurements Open
The effect of increased temperature on Staphylococcus aureus during the inoculation step of the agar dilution plate count method was investigated as a possible cause of artificially high persister counts. For some isolates, exposure of the…
View article: Ampicillin killing curve patterns of Haemophilus influenzae type b isolates by agar dilution plate count method
Ampicillin killing curve patterns of Haemophilus influenzae type b isolates by agar dilution plate count method Open
The responses of 20 ampicillin-susceptible Haemophilus influenzae type b clinical isolates to the bactericidal action of ampicillin were studied by using a modified agar dilution plate count method. A well-defined paradoxical effect was ob…
View article: Oxacillin killing curve patterns of Staphylococcus aureus isolates by agar dilution plate count method
Oxacillin killing curve patterns of Staphylococcus aureus isolates by agar dilution plate count method Open
The bactericidal dynamics of oxacillin against four Staphylococcus aureus isolates with known 24-h "persister" percentages were studied by using the agar dilution plate count method. Isolates were selected to provide a representative spect…
View article: Influence of technical factor variations during inoculum preparation on the agar dilution plate-count method for quantitation of Staphylococcus aureus oxacillin persisters
Influence of technical factor variations during inoculum preparation on the agar dilution plate-count method for quantitation of Staphylococcus aureus oxacillin persisters Open
The possible introduction of error into Staphylococcus aureus oxacillin intrinsic persister measurements by the agar dilution plate-count method resulting from technical factor variations associated with inoculum preparation was investigat…
View article: Influence of technical factor variations on serum inhibition and bactericidal titers
Influence of technical factor variations on serum inhibition and bactericidal titers Open
The influence of technical factor variations on serum bactericidal and serum inhibitory titers was studied by using Staphylococcus aureus clinical isolates versus oxacillin-spiked human serum. Parallel tests, both with and without the use …
View article: Evaluation of the AutoMicrobic system Gram-Positive Susceptibility-MIC card for detection of oxacillin-resistant coagulase-negative staphylococci
Evaluation of the AutoMicrobic system Gram-Positive Susceptibility-MIC card for detection of oxacillin-resistant coagulase-negative staphylococci Open
A total of 398 consecutive clinical staphylococcus isolates, of which 205 were coagulase negative and 193 were coagulase positive, were tested in parallel by using AutoMicrobic system Gram-Positive Susceptibility-MIC cards and modified Mue…
View article: Evaluation of the newly modified AutoMicrobic system gram-positive susceptibility-MIC card for detection of methicillin-resistant Staphylococcus aureus
Evaluation of the newly modified AutoMicrobic system gram-positive susceptibility-MIC card for detection of methicillin-resistant Staphylococcus aureus Open
AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) gram-positive susceptibility-MIC (GPS-MIC) cards and GPS cards were compared for their ability to detect methicillin-resistant Staphylococcus aureus (MRSA) in two groups of isolates…
View article: Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus
Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus Open
We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin …
View article: Evaluation of oxacillin tolerance in Staphylococcus aureus by a novel method
Evaluation of oxacillin tolerance in Staphylococcus aureus by a novel method Open
A novel agar dilution plate-count procedure for the quantitative measurement of bacterial inhibition and killing is described. For Staphylococcus aureus versus oxacillin, by the agar dilution plate-count procedure it was found that only 1 …
View article: Evaluation of the AutoMicrobic system for identification and susceptibility testing of gram-negative bacilli
Evaluation of the AutoMicrobic system for identification and susceptibility testing of gram-negative bacilli Open
The AutoMicrobic system (AMS) (Vitek Systems, Inc., Hazelwood, Mo.) was compared with the API-20E system for the identification of gram-negative bacilli by using 380 stock clinical isolates and 377 immediately encountered fresh clinical is…
View article: Evaluation of the automicrobic system for susceptibility testing of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin
Evaluation of the automicrobic system for susceptibility testing of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin Open
The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were…
View article: Evaluation of the automicrobic system for detection of resistance of Staphylococcus aureus to methicillin
Evaluation of the automicrobic system for detection of resistance of Staphylococcus aureus to methicillin Open
The AutoMicrobic system (AMS) (Vitek System, Inc., Hazelwood, Mo.) was tested for its ability to determine oxacillin and gentamicin susceptibility of 98 known oxacillin-susceptible and 103 known oxacillin-resistant Staphylococcus aureus is…
View article: Error rates associated with the use of recently proposed breakpoints for testing Pseudomonas aeruginosa versus gentamicin, tobramycin, and amikacin by the standardized disk agar diffusion test
Error rates associated with the use of recently proposed breakpoints for testing Pseudomonas aeruginosa versus gentamicin, tobramycin, and amikacin by the standardized disk agar diffusion test Open
Two hundred fifteen Pseudomonas aeruginosa isolates were tested in parallel by the disk agar diffusion test, using a standardized agar preparation, and by a microbroth test, using dilutions differing by small arithmetic increments. For gen…
View article: Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification
Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification Open
To evaluate the accuracy and cost effectiveness of the AutoSCAN-3 (Micro-Scan Systems of America, Sacramento, Calif.) and Sceptor (BBL Microbiology Systems, Cockeysville, Md.) systems for identification of members of the Enterobacteriaceae…
View article: Comparative evaluation of the micro-media system, sceptor, and MIC-2000 microdilution methods for testing Pseudomonas aeruginosa against gentamicin, tobramycin, and amikacin
Comparative evaluation of the micro-media system, sceptor, and MIC-2000 microdilution methods for testing Pseudomonas aeruginosa against gentamicin, tobramycin, and amikacin Open
Minimum inhibitory concentrations (MICs) for selected strains of Pseudomonas aeruginosa versus gentamicin, tobramycin, and amikacin were replicated in parallel with MMS (Micro-Media Systems, Potomac, Md). Sceptor (BBL Microbiology Systems,…
View article: Broth microdilution testing of Pseudomonas aeruginosa and aminoglycosides: need for employing dilutions differing by small arithmetic increments
Broth microdilution testing of Pseudomonas aeruginosa and aminoglycosides: need for employing dilutions differing by small arithmetic increments Open
The use of dilutions differing by small arithmetic increments was studied as a means for improving the definition and measurement of minimum inhibitory concentrations and precision parameters for testing Pseudomonas aeruginosa versus the a…