Richard Sparling
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View article: Genomic Drivers of Biofilm Formation in Salmonella Enteritidis and S. Kentucky from Poultry Production
Genomic Drivers of Biofilm Formation in Salmonella Enteritidis and S. Kentucky from Poultry Production Open
Salmonella Enteritidis (SE) remains a leading cause of human illness worldwide, and its persistence in poultry environments might be partially attributed to their ability to form biofilm. This study compared the biofilm capacity of 15 SE a…
View article: Microbial consortia in full-scale pre-ozonated biologically active filters treating a high-DOC water: Effects of seasonal and operational variations
Microbial consortia in full-scale pre-ozonated biologically active filters treating a high-DOC water: Effects of seasonal and operational variations Open
This study investigated the microbial dynamics in full-scale biologically active anthracite/silica sand (BAS) and granular activated carbon (BAC) filters used to treat high-DOC coagulated softened water. By analyzing these filter types acr…
View article: <i>Burkholderia</i> Genes Involved in Extracellular Bioplastic Degradation Revealed by Tn-seq and CRISPR-Cas
<i>Burkholderia</i> Genes Involved in Extracellular Bioplastic Degradation Revealed by Tn-seq and CRISPR-Cas Open
Bioplastics represent promising alternatives to petroleum-based plastics, yet their biodegradation remains insufficiently understood. Identifying bacteria capable of degrading bioplastics extracellularly could enhance end-of-life managemen…
View article: Extracellular electron supply from titanium( <scp>III</scp> )nitrilotriacetic acid promotes production of reduced fermentation end products in <i>Clostridium ljungdahlii</i>
Extracellular electron supply from titanium( <span>III</span> )nitrilotriacetic acid promotes production of reduced fermentation end products in <i>Clostridium ljungdahlii</i> Open
Interest in the microbial production of bioethanol from renewable sources has prompted efforts to improve its production in homoacetogenic bacteria. It has been suggested that a strongly reducing environment might support the formation of …
View article: Kinetic analysis of phenotypic and growth variations in <i>Clostridium autoethanogenum</i> across two xylose concentration ranges
Kinetic analysis of phenotypic and growth variations in <i>Clostridium autoethanogenum</i> across two xylose concentration ranges Open
This study investigated the heterotrophic growth kinetics of Clostridium autoethanogenum in batch cultures using xylose, a hemicellulose hydrolysis product, as the fermentation substrate. The growth data were modeled using the Monod equati…
View article: Effects of Ozone and Biological Degradation on the Removal and Transformation of Highly Hydrophilic DOC in a Conventional Water Treatment Process
Effects of Ozone and Biological Degradation on the Removal and Transformation of Highly Hydrophilic DOC in a Conventional Water Treatment Process Open
View article: Adaptive evolution of <i>Clostridium thermocellum</i> ATCC 27405 on alternate carbon sources leads to altered fermentation profiles
Adaptive evolution of <i>Clostridium thermocellum</i> ATCC 27405 on alternate carbon sources leads to altered fermentation profiles Open
Consolidated bioprocessing candidate, Clostridium thermocellum, is a cellulose hydrolysis specialist, with the ability to ferment the released sugars to produce bioethanol. C. thermocellum is generally studied with model substrates Avicel …
View article: Semi‐continuous non‐sterile production of medium chain‐length polyhydroxyalkanoates from fatty acids
Semi‐continuous non‐sterile production of medium chain‐length polyhydroxyalkanoates from fatty acids Open
Medium chain‐length polyhydroxyalkanoates (mcl‐PHAs) are efficiently synthesized from fatty acids. It was hypothesized that under non‐axenic conditions, the anti‐microbial properties of fatty acids would reduce growth of microbial competit…
View article: Bioremediation of Chlorate and Chromium in Soil Columns Using Contaminated Site Native Culture
Bioremediation of Chlorate and Chromium in Soil Columns Using Contaminated Site Native Culture Open
Chlorate and hexavalent chromium (chromate) are both widely used in different industries, and the improper waste management in the past left many sites with elevated concentrations in groundwater that pose potential risk to human and/or ec…
View article: Metagenomic comparison of effects of mesophilic and thermophilic manure anaerobic digestion on antimicrobial resistance genes and mobile genetic elements
Metagenomic comparison of effects of mesophilic and thermophilic manure anaerobic digestion on antimicrobial resistance genes and mobile genetic elements Open
This study evaluated the long-term effects of mesophilic (MAD) and thermophilic (TAD) anaerobic digestion on the fate and evolution of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in cattle manure following a me…
View article: Influence of three different manure treatments on antimicrobial resistance genes and mobile genetic elements
Influence of three different manure treatments on antimicrobial resistance genes and mobile genetic elements Open
There is a growing concern about the potential dissemination of antimicrobial resistance into agricultural fields due to the application of manure as crop fertilizer. While reducing the usage of antibiotics in livestock production stands a…
View article: The interactive effect of environmental factors on methane oxidation in a continental landfill bio-cover
The interactive effect of environmental factors on methane oxidation in a continental landfill bio-cover Open
In the current study, the interactive effect of environmental factors was studied in an active bio-cover after two years of operation and experiencing a wide range of temperatures across the seasons (45 ℃) in a continental climate conditio…
View article: Bioremediation of Chlorate and Chromium Contamination with Native Microbial Culture in Cold Climate
Bioremediation of Chlorate and Chromium Contamination with Native Microbial Culture in Cold Climate Open
Chlorate and hexavalent chromium are two chemicals with adverse health effects that may cause groundwater contamination in industrial areas. The objective of this study was to determine if the native microorganisms collected from a site co…
View article: Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles
Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles Open
View article: Metagenomic analyses reveal that mesophilic anaerobic digestion substantially reduces the abundance of antibiotic resistance genes and mobile genetic elements in dairy manures
Metagenomic analyses reveal that mesophilic anaerobic digestion substantially reduces the abundance of antibiotic resistance genes and mobile genetic elements in dairy manures Open
Animal manure is considered one of the main reservoirs of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) that carry them. There is increasing concern regarding the dissemination of antibiotic resistance due to the ap…
View article: Metagenomic Comparison of Effects of Mesophilic and Thermophilic Manure Anaerobic Digestion on Antimicrobial Resistance Genes and Mobile Genetic Elements
Metagenomic Comparison of Effects of Mesophilic and Thermophilic Manure Anaerobic Digestion on Antimicrobial Resistance Genes and Mobile Genetic Elements Open
View article: Metagenomic Analyses Reveal that Mesophilic Anaerobic Digestion Substantially Reduces the Abundance of Antibiotic Resistance Genes and Mobile Genetic Elements in Dairy Manures
Metagenomic Analyses Reveal that Mesophilic Anaerobic Digestion Substantially Reduces the Abundance of Antibiotic Resistance Genes and Mobile Genetic Elements in Dairy Manures Open
View article: A meta-analysis reveals that operational parameters influence levels of antibiotic resistance genes during anaerobic digestion of animal manures
A meta-analysis reveals that operational parameters influence levels of antibiotic resistance genes during anaerobic digestion of animal manures Open
Anaerobic digestion (AD) has shown the potential to reduce the numbers and types of antibiotic-resistance genes (ARG) present in animal manures. However, the variability of the results has limited the ability to draw solid conclusions. To …
View article: Analysis of the <i>Yarrowia</i> <i>lipolytica</i> proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions
Analysis of the <i>Yarrowia</i> <i>lipolytica</i> proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions Open
Oleaginous yeasts have the ability to store greater than 20% of their mass as neutral lipids, in the form of triacylglycerides. The ATP citrate lyase is thought to play a key role in triacylglyceride synthesis, but the relationship between…
View article: A Meta-Analysis Reveals that Operational Parameters Influence Levels of Antibiotic Resistance Genes During Anaerobic Digestion of Animal Manures
A Meta-Analysis Reveals that Operational Parameters Influence Levels of Antibiotic Resistance Genes During Anaerobic Digestion of Animal Manures Open
View article: Response of Microbial Community to Induced Failure of Anaerobic Digesters Through Overloading With Propionic Acid Followed by Process Recovery
Response of Microbial Community to Induced Failure of Anaerobic Digesters Through Overloading With Propionic Acid Followed by Process Recovery Open
In order to effectively use microbial-based strategies to manage anaerobic digesters, it is necessary to distinguish between community shifts that are part of the natural dynamic of the system and shifts caused by environmental or operatio…
View article: Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing
Digestibility of Wheat and Cattail Biomass Using a Co-culture of Thermophilic Anaerobes for Consolidated Bioprocessing Open
View article: Rheological Behavior of High Cell Density Pseudomonas putida LS46 Cultures during Production of Medium Chain Length Polyhydroxyalkanoate (PHA) Polymers
Rheological Behavior of High Cell Density Pseudomonas putida LS46 Cultures during Production of Medium Chain Length Polyhydroxyalkanoate (PHA) Polymers Open
The rheology of high-cell density (HCD) cultures is an important parameter for its impact on mixing and sparging, process scale-up, and downstream unit operations in bioprocess development. In this work, time-dependent rheological properti…
View article: Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46
Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46 Open
High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen…
View article: A novel thermostable GH5 β-xylosidase from Thermogemmatispora sp. T81
A novel thermostable GH5 β-xylosidase from Thermogemmatispora sp. T81 Open
View article: Enhanced depolymerization and utilization of raw lignocellulosic material by co-cultures of <i>Ruminiclostridium thermocellum</i> with hemicellulose-utilizing partners
Enhanced depolymerization and utilization of raw lignocellulosic material by co-cultures of <i>Ruminiclostridium thermocellum</i> with hemicellulose-utilizing partners Open
Ruminiclostridium thermocellum is one of the most promising candidates for consolidated bioprocessing (CBP) of low-cost lignocellulosic materials to biofuels but it still shows poor performance in its ability to deconstruct untreated ligno…
View article: Analysis of carbohydrate-active enzymes in <i>Thermogemmatispora</i> sp. strain T81 reveals carbohydrate degradation ability
Analysis of carbohydrate-active enzymes in <i>Thermogemmatispora</i> sp. strain T81 reveals carbohydrate degradation ability Open
The phylum Chloroflexi is phylogenetically diverse and is a deeply branching lineage of bacteria that express a broad spectrum of physiological and metabolic capabilities. Members of the order Ktedonobacteriales, including the families Kte…
View article: Cofactor Tail Length Modulates Catalysis of Bacterial F420-Dependent Oxidoreductases
Cofactor Tail Length Modulates Catalysis of Bacterial F420-Dependent Oxidoreductases Open
F420 is a microbial cofactor that mediates a wide range of physiologically important and industrially relevant redox reactions, including in methanogenesis and tetracycline biosynthesis. This deazaflavin comprises a redox-active…
View article: Mixotrophy drives niche expansion of verrucomicrobial methanotrophs
Mixotrophy drives niche expansion of verrucomicrobial methanotrophs Open
Aerobic methanotrophic bacteria have evolved a specialist lifestyle dependent on consumption of methane and other short-chain carbon compounds. However, their apparent substrate specialism runs contrary to the high relative abundance of th…
View article: Aerobic H<sub>2</sub>respiration enhances metabolic flexibility of methanotrophic bacteria
Aerobic H<sub>2</sub>respiration enhances metabolic flexibility of methanotrophic bacteria Open
Methanotrophic bacteria are important soil biofilters for the climate-active gas methane. The prevailing opinion is that these bacteria exclusively metabolise single-carbon, and in limited instances, short-chain hydrocarbons for growth. Th…