Richard J. Ward
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View article: Pyroptosis and the fight against lung cancer
Pyroptosis and the fight against lung cancer Open
Pyroptosis, a newly characterized type of inflammatory programmed cell death (PCD), is usually triggered by multiple inflammasomes which can recognize different danger or damage‐associated molecular patterns (DAMPs), leading to the activat…
View article: Diffusion and Oligomerization States of the Muscarinic M1 Receptor in Live Cells − The Impact of Ligands and Membrane Disruptors
Diffusion and Oligomerization States of the Muscarinic M1 Receptor in Live Cells − The Impact of Ligands and Membrane Disruptors Open
G protein-coupled receptors (GPCRs) are a major gateway to cellular signaling, which respond to ligands binding at extracellular sites through allosteric conformational changes that modulate their interactions with G proteins and arrestins…
View article: Biocatalytic potential of Pseudolycoriella CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides
Biocatalytic potential of Pseudolycoriella CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides Open
View article: The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures
The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures Open
Ferulic acid has antioxidant properties of interest to the food industry and can be released from natural plant fibres using feruloyl esterases. Esterases active at high temperatures are highly desirable but currently underrepresented. Her…
View article: Selective phosphorylation of threonine residues defines GPR84–arrestin interactions of biased ligands
Selective phosphorylation of threonine residues defines GPR84–arrestin interactions of biased ligands Open
View article: Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways
Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways Open
Dictamnine (Dic), a naturally occurring small-molecule furoquinoline alkaloid isolated from the root bark of Dictamnus dasycarpus Turcz., is reported to display anticancer properties. However, little is known about the direct target protei…
View article: The monocyte-to-lymphocyte ratio: Sex-specific differences in the tuberculosis disease spectrum, diagnostic indices and defining normal ranges
The monocyte-to-lymphocyte ratio: Sex-specific differences in the tuberculosis disease spectrum, diagnostic indices and defining normal ranges Open
Background The monocyte-to-lymphocyte ratio (MLR) has been advocated as a biomarker in tuberculosis. Our objective was to evaluate its clinical value and associations. Methods Blood counts, inflammatory markers and clinical parameters were…
View article: The C-terminal of the α1b-adreneroceptor is a key determinant for its structure integrity and biological functions
The C-terminal of the α1b-adreneroceptor is a key determinant for its structure integrity and biological functions Open
The C-terminal of G protein-coupled receptors is now recognized as being important for G protein activation and signaling function. To detect the role of C-terminal tail in receptor activation, we used the α1b-AR, which has a long C-termin…
View article: The monocyte-to-lymphocyte ratio: defining a normal range, sex-specific differences in the tuberculosis disease spectrum and diagnostic indices
The monocyte-to-lymphocyte ratio: defining a normal range, sex-specific differences in the tuberculosis disease spectrum and diagnostic indices Open
Background The monocyte-to-lymphocyte ratio has been advocated as a biomarker in tuberculosis. Our objective was to evaluate its clinical role in diagnosis, prognosis and treatment outcome. Methods Complete blood counts from an unselected …
View article: Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold
Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed β-3-prism scaffold Open
Effective use of plant biomass as an abundant and renewable feedstock for biofuel production and biorefinery requires efficient enzymatic mobilization of cell wall polymers. Knowledge of plant cell wall composition and architecture has bee…
View article: Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t
Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t Open
View article: Recombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources
Recombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources Open
View article: Figure 5 A
Figure 5 A Open
Confocal images used to generate spatial intensity distribution analysis data for the publication figure 5 A
View article: Figure 5 B
Figure 5 B Open
Confocal images used to generate spatial intensity distribution analysis data for the publication figure 5 B
View article: Figure 3 A
Figure 3 A Open
Confocal images used to generate spatial intensity distribution analysis data for the publication figure 3 A
View article: Figure 6 B
Figure 6 B Open
Confocal images used to generate spatial intensity distribution analysis data for the publication figure 6 B
View article: Figure 3 F
Figure 3 F Open
Confocal images used to generate spatial intensity distribution analysis data for the publication figure 3 F
View article: Quantifying membrane protein oligomerization using two-dimensional fluorescence intensity fluctuation spectrometry
Quantifying membrane protein oligomerization using two-dimensional fluorescence intensity fluctuation spectrometry Open
Current methods for determining membrane protein association in cells are either very time consuming, require complicated procedures, or lack the sensitivity needed to assess the effect of concentration or ligand binding on the observed ol…
View article: A general method to quantify ligand-driven oligomerization from fluorescence-based images
A general method to quantify ligand-driven oligomerization from fluorescence-based images Open
View article: Design, Synthesis, and Evaluation of a Diazirine Photoaffinity Probe for Ligand-Based Receptor Capture Targeting G Protein–Coupled Receptors
Design, Synthesis, and Evaluation of a Diazirine Photoaffinity Probe for Ligand-Based Receptor Capture Targeting G Protein–Coupled Receptors Open
View article: A general method to quantify ligand-driven oligomerization using single- or two-photon excitation microscopy
A general method to quantify ligand-driven oligomerization using single- or two-photon excitation microscopy Open
Current technologies for probing membrane protein association and stability in cells are either very laborious or lack the bandwidth needed for fully quantitative analysis. Here we introduce a platform, termed one- or two-dimensional fluor…
View article: GPCR homo-oligomerization
GPCR homo-oligomerization Open
G protein-coupled receptors (GPCRs) are an extensive class of trans-plasma membrane proteins that function to regulate a wide range of physiological functions. Despite a general perception that GPCRs exist as monomers an extensive literatu…
View article: Nutrient and drought stress: implications for phenology and biomass quality in miscanthus
Nutrient and drought stress: implications for phenology and biomass quality in miscanthus Open
Background and Aims The cultivation of dedicated biomass crops, including miscanthus, on marginal land provides a promising approach to the reduction of dependency on fossil fuels. However, little is known about the impact of environmental…
View article: A Molecular Basis for Selective Antagonist Destabilization of Dopamine D3 Receptor Quaternary Organization
A Molecular Basis for Selective Antagonist Destabilization of Dopamine D3 Receptor Quaternary Organization Open
View article: Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor
Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor Open
Previous studies have indicated that the G-protein-coupled secretin receptor is present as a homodimer, organized through symmetrical contacts in transmembrane domain IV, and that receptor dimerization is critical for high-potency signalli…
View article: Methods used to study the oligomeric structure of G-protein-coupled receptors
Methods used to study the oligomeric structure of G-protein-coupled receptors Open
G-protein-coupled receptors (GPCRs), which constitute the largest family of cell surface receptors, were originally thought to function as monomers, but are now recognized as being able to act in a wide range of oligomeric states and indee…
View article: Issue Information ‐ TOC
Issue Information ‐ TOC Open
View article: A Neurospora crassa ÿ-glucosidase with potential for lignocellulose hydrolysis shows strong glucose tolerance and stimulation by glucose and xylose
A Neurospora crassa ÿ-glucosidase with potential for lignocellulose hydrolysis shows strong glucose tolerance and stimulation by glucose and xylose Open
View article: Regulation of Oligomeric Organization of the Serotonin 5-Hydroxytryptamine 2C (5-HT2C) Receptor Observed by Spatial Intensity Distribution Analysis
Regulation of Oligomeric Organization of the Serotonin 5-Hydroxytryptamine 2C (5-HT2C) Receptor Observed by Spatial Intensity Distribution Analysis Open
View article: Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis
Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis Open